Cited by 42RNA Research and SplicingHeat shock proteins researchFungal and yeast genetics research

The intron-containing hsp82 gene of the dimorphic pathogenic fungus Histoplasma capsulatum is properly spliced in severe heat shock conditions.

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Minchiotti, G, Gargano, S, Maresca, B·1991·Molecular and Cellular Biology

Abstract

We have isolated and characterized a heat-inducible gene, hsp82, from the dimorphic pathogenic fungus Histoplasma capsulatum, which is a filamentous mold at 25 degrees C and a unicellular yeast at 37 degrees C. This gene, which has a high degree of homology with other members of the hsp82 gene family, is split into three exons and two introns of 122 and 86 nucleotides, respectively. Contrary to what has been demonstrated in Drosophila melanogaster, Saccharomyces cerevisiae, and other organisms, hsp82 mRNA in H. capsulatum is properly spliced during the severe heat conditions of 37 to 40 degrees C in the temperature-sensitive Downs strain. Splicing accuracy was also observed at 42 degrees C in the temperature-tolerant G222B strain, which showed no evidence of accumulation of primary transcripts. Furthermore, the intron containing the beta-tubulin gene is also properly spliced at the upper temperature range, suggesting that the lack of a block in splicing may be a general phenomenon in this organism.

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Crossref · 10.1128/mcb.11.11.5624

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Authors
Minchiotti, G., Gargano, S., & Maresca, B.
Affiliations
  • Institute of Genetics and Biophysics (IT)
  • Washington University in St. Louis (US)
Venue
Molecular and Cellular Biology
Published
1991-11
Volume
11
Issue
11
Pages
5624-5630
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
OpenAlex
W2235302637
Cited by
42
References
43
Citations by year
2018: 1 · 2017: 1 · 2016: 1 · 2013: 1 · 2012: 2
FWCI
1.72
Type
journal article
Language
en
Open access
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institutes of Health (5r01ai029609-05)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords
Histoplasma capsulatum, heat shock, pre-mRNA splicing, introns, dimorphic fungi, beta-tubulin gene, heat tolerance, gene structure
MeSH terms
Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Fungal, Genes, Fungal, Hot Temperature, Heat-Shock Proteins, Histoplasma, Introns, Molecular Sequence Data, Oligonucleotides, RNA Splicing, RNA, Messenger, Transcription, Genetic, Tubulin, Blotting, Southern

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