The intron-containing hsp82 gene of the dimorphic pathogenic fungus Histoplasma capsulatum is properly spliced in severe heat shock conditions.
Open accessAbstract
We have isolated and characterized a heat-inducible gene, hsp82, from the dimorphic pathogenic fungus Histoplasma capsulatum, which is a filamentous mold at 25 degrees C and a unicellular yeast at 37 degrees C. This gene, which has a high degree of homology with other members of the hsp82 gene family, is split into three exons and two introns of 122 and 86 nucleotides, respectively. Contrary to what has been demonstrated in Drosophila melanogaster, Saccharomyces cerevisiae, and other organisms, hsp82 mRNA in H. capsulatum is properly spliced during the severe heat conditions of 37 to 40 degrees C in the temperature-sensitive Downs strain. Splicing accuracy was also observed at 42 degrees C in the temperature-tolerant G222B strain, which showed no evidence of accumulation of primary transcripts. Furthermore, the intron containing the beta-tubulin gene is also properly spliced at the upper temperature range, suggesting that the lack of a block in splicing may be a general phenomenon in this organism.
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Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589 · see citing works
Molecular cloning: A laboratory manual
Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684 · see citing works
Yanisch-Perron, C., Vieira, J., & Messing, J. W. (1985) · Gene · doi:10.1016/0378-1119(85)90120-9 · Cited by 14,868 · see citing works
Lindquist, S., & Craig, E. A. (1988) · Annual Review of Genetics · doi:10.1146/annurev.ge.22.120188.003215 · Cited by 5,304 · see citing works
Lindquist, S. L. (1986) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.55.070186.005443 · Cited by 4,611 · see citing works
A catalogue of splice junction sequences
Mount, S. M. (1982) · Nucleic Acids Research · doi:10.1093/nar/10.2.459 · Cited by 3,881 · see citing works
The scanning model for translation: an update.
Kozak, M. (1989) · The Journal of Cell Biology · doi:10.1083/jcb.108.2.229 · Cited by 3,485 · see citing works
Dideoxy sequencing method using denatured plasmid templates
Hattori, M., & Sakaki, Y. (1986) · Analytical Biochemistry · doi:10.1016/0003-2697(86)90403-3 · Cited by 1,469 · see citing works
Schlesinger, M. J. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)38314-0 · Cited by 1,362 · see citing works
The induction of gene activity in drosophila by heat shock
Ashburner, M., & Bonner, J. J. (1979) · Cell · doi:10.1016/0092-8674(79)90150-8 · Cited by 1,322 · see citing works
Key features of heat shock regulatory elements.
Αmin, J., Ananthan, J., & Voellmy, R. W. (1988) · Molecular and Cellular Biology · doi:10.1128/mcb.8.9.3761 · Cited by 572 · see citing works
RNA splicing is interrupted by heat shock and is rescued by heat shock protein synthesis
Yost, H. J., & Lindquist, S. L. (1986) · Cell · doi:10.1016/0092-8674(86)90382-x · Cited by 513 · see citing works
Identification and Comparison of Stable and Unstable mRNAs in Saccharomyces cerevisiae
Herrick, D., Parker, R., & Jacobson, A. (1990) · Molecular and Cellular Biology · doi:10.1128/mcb.10.5.2269 · Cited by 406 · see citing works
Langford, C. J., Klinz, F., Donath, C., & Gallwitz, D. (1984) · Cell · doi:10.1016/0092-8674(84)90344-1 · Cited by 330 · see citing works
Activation of heat-shock genes in eukaryotes
Pelham, H. R. B. (1985) · Trends in Genetics · doi:10.1016/0168-9525(85)90012-5 · Cited by 265 · see citing works
Sequence and regulation of a gene encoding a human 89-kilodalton heat shock protein.
Hickey, E. D., Brandon, S. E., Smale, G., et al. (1989) · Molecular and Cellular Biology · doi:10.1128/mcb.9.6.2615 · Cited by 240 · see citing works
Heat Shock Genes: Regulatory Role for Differentiation in Parasitic Protozoa
der Ploeg, L. H. T. V., Giannini, S. H., & Cantor, C. R. (1985) · Science · doi:10.1126/science.4012301 · Cited by 228 · see citing works
Blackman, R. K., & Meselson, M. S. (1986) · Journal of Molecular Biology · doi:10.1016/s0022-2836(86)80001-8 · Cited by 223 · see citing works
Key Features of Heat Shock Regulatory Elements
Αmin, J., Ananthan, J., & Voellmy, R. W. (1988) · Molecular and Cellular Biology · doi:10.1128/mcb.8.9.3761-3769.1988 · Cited by 197 · see citing works
Complete sequence of the heat shock-inducible HSP90 gene of Saccharomyces cerevisiae.
Farrelly, F. W., & FINKELSTEIN, D. B. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)91077-x · Cited by 188 · see citing works
Bond, U. (1988) · The EMBO Journal · doi:10.1002/j.1460-2075.1988.tb03227.x · Cited by 174 · see citing works
Maresca, B., & Kobayashi, G. S. (1989) · Microbiological Reviews · doi:10.1128/mr.53.2.186-209.1989 · Cited by 169 · see citing works
Self-citationNucleotide sequence of a cDNA for a member of the human 90-kDa heat-shock protein family
Rebbe, N. F., Ware, J. L., Bertina, R. M., Modrich, P. L., & Stafford, D. W. (1987) · Gene · doi:10.1016/0378-1119(87)90012-6 · Cited by 166 · see citing works
Moore, S. K., Kozak, C. A., Robinson, E. A., Ullrich, S. J., & Appella, E. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)83551-7 · Cited by 149 · see citing works
RNA metabolism: strategies for regulation in the heat shock response
Yost, H. J., Petersen, R. B., & Linquist, S. (1990) · Trends in Genetics · doi:10.1016/0168-9525(90)90183-7 · Cited by 145 · see citing works
Yost, H. J., & Lindquist, S. (1991) · Molecular and Cellular Biology · doi:10.1128/mcb.11.2.1062 · Cited by 123 · see citing works
Heat-Shock Proteins And Development
Bond, U., & Schlesinger, M. J. (1987) · Advances in genetics · doi:10.1016/s0065-2660(08)60005-x · Cited by 105 · see citing works
Translation of Unspliced Transcripts After Heat Shock
Yost, H. J., & Lindquist, S. L. (1988) · Science · doi:10.1126/science.3201243 · Cited by 103 · see citing works
Maresca, B., & Kobayashi, G. S. (1989) · Microbiological Reviews · doi:10.1128/mmbr.53.2.186-209.1989 · Cited by 89 · see citing works
Self-citationMorphogenesis and pathogenicity of Histoplasma capsulatum
Medoff, G., Kobayashi, G. S., Painter, A. A., et al. (1987) · Infection and Immunity · doi:10.1128/iai.55.6.1355-1358.1987 · Cited by 88 · see citing works
Medoff, G., Maresca, B., Lambowitz, A. M., et al. (1986) · Journal of Clinical Investigation · doi:10.1172/jci112757 · Cited by 79 · see citing works
Self-citationLambowitz, A. M., Kobayashi, G. S., Painter, A. A., et al. (1983) · Nature · doi:10.1038/303806a0 · Cited by 78 · see citing works
Effect of heat shock on RNA metabolism in HeLa cells
Sadis, S. E., Hickey, E. D., & Weber, L. A. (1988) · Journal of Cellular Physiology · doi:10.1002/jcp.1041350304 · Cited by 76 · see citing works
Caruso, M., Sacco, M., Medoff, G., et al. (1987) · Molecular Microbiology · doi:10.1111/j.1365-2958.1987.tb00507.x · Cited by 71 · see citing works
Kay, R. J., Russnak, R., Jones, D., et al. (1987) · Nucleic Acids Research · doi:10.1093/nar/15.9.3723 · Cited by 54 · see citing works
Cloning of the chick hsp 90 cDNA in expression vector
CATELLI, M. G., Binart, N., Feramisco, J. R., & Helfman, D. M. (1985) · Nucleic Acids Research · doi:10.1093/nar/13.17.6035 · Cited by 54 · see citing works
Coccidioidomycosis and histoplasmosis a review of their epidemiology and geographical distribution
Ajello, L. (1971) · Mycopathologia · doi:10.1007/bf02051969 · Cited by 49 · see citing works
Synthesis of trypanosome hsp70 mRNA is resistant to disruption of trans-splicing by heat shock
Muhich, M. L., & Boothroyd, J. C. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)83206-9 · Cited by 43 · see citing works
RNA Metabolism during Morphogenesis in Histoplasma capsulatum
CHEUNG, S.-S. C., Kobayashi, G. S., Schlessinger, D., et al. (1974) · Journal of General Microbiology · doi:10.1099/00221287-82-2-301 · Cited by 41 · see citing works
Shearer, G., Birge, C. H., Yuckenberg, P. D., et al. (1987) · Microbiology · doi:10.1099/00221287-133-12-3375 · Cited by 40 · see citing works
Characterization of Alpha and Beta Tubulin Genes in the Dimorphic Fungus Histoplasma capsulatum
Harris, G. S., Keath, E. J., & Medoff, J. (1989) · Microbiology · doi:10.1099/00221287-135-7-1817 · Cited by 26 · see citing works
Expression of α- and β-Tubulin Genes during Dimorphic-Phase Transitions of Histoplasma capsulation
Harris, G. S., Keath, E. J., & Medoff, J. (1989) · Molecular and Cellular Biology · doi:10.1128/mcb.9.5.2042-2049.1989 · Cited by 21 · see citing works
Cloning of the chick hsp 90 cDNA in expression vector
CATELLI, M. G., Binart, N., Feramisco, J. R., et al. (1985) · Nucleic Acids Research · doi:10.1093/nar/13.20.7541 · Cited by 6 · see citing works
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Verghese, J., Abrams, J., Wang, Y., & Morano, K. A. (2012) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.05018-11 · Cited by 575 · see citing works
Fungal Hsp90: a biological transistor that tunes cellular outputs to thermal inputs
Leach, M. D., Klipp, E., Cowen, L. E., & Brown, A. J. P. (2012) · Nature Reviews Microbiology · doi:10.1038/nrmicro2875 · Cited by 107 · see citing works
The relevance of heat shock regulation in fungal pathogens of humans
Brown, A. J. P., Leach, M. D., & Nicholls, S. (2010) · Virulence · doi:10.4161/viru.1.4.12364 · Cited by 30 · see citing works
- Authors
- Minchiotti, G., Gargano, S., & Maresca, B.
- Affiliations
- Institute of Genetics and Biophysics (IT)
- Washington University in St. Louis (US)
- ORCID
- Minchiotti, G
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1991-11
- Volume
- 11
- Issue
- 11
- Pages
- 5624-5630
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC361933
- OpenAlex
- W2235302637
- Cited by
- 42
- References
- 43
- Citations by year
- 2018: 1 · 2017: 1 · 2016: 1 · 2013: 1 · 2012: 2
- FWCI
- 1.72
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Cellular Biology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ai029609-05)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Histoplasma capsulatum, heat shock, pre-mRNA splicing, introns, dimorphic fungi, beta-tubulin gene, heat tolerance, gene structure
- MeSH terms
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Fungal, Genes, Fungal, Hot Temperature, Heat-Shock Proteins, Histoplasma, Introns, Molecular Sequence Data, Oligonucleotides, RNA Splicing, RNA, Messenger, Transcription, Genetic, Tubulin, Blotting, Southern
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