The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
A group of scs elements function as domain boundaries in an enhancer-blocking assay.
Open accessAbstract
Chromosomes of higher eukaryotes are thought to be organized into a series of discrete and topologically independent higher-order domains. In addition to providing a mechanism for chromatin compaction, these higher-order domains are thought to define independent units of gene activity. Implicit in most models for the folding of the chromatin fiber are special nucleoprotein structures, the domain boundaries, which serve to delimit each higher-order chromosomal domain. We have used an "enhancer-blocking assay" to test putative domain boundaries for boundary function in vivo. This assay is based on the notion that in delimiting independent units of gene activity, domain boundaries should be able to restrict the scope of activity of enhancer elements to genes which reside within the same domain. In this case, interposing a boundary between an enhancer and a promoter should block the action of the enhancer. In the experiments reported here, we have used the yolk protein-1 enhancer element and an hsp70 promoter:lacZ fusion gene to test putative boundary DNA segments for enhancer-blocking activity. We have found that several scs-like elements are capable of blocking the action of the yp-1 enhancer when placed between it and the hsp70 promoter. In contrast, a MAR/SAR DNA segment and another spacer DNA segment had no apparent effect on enhancer activity.
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Cites
Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold
Mirkovitch, J., Mirault, M.-E., & Laemmli, U. K. (1984) · Cell · doi:10.1016/0092-8674(84)90208-3 · Cited by 1,066
Cockerill, P. N., & Garrard, W. T. (1986) · Cell · doi:10.1016/0092-8674(86)90761-0 · Cited by 881
Detection in situ of genomic regulatory elements in Drosophila.
O'Kane, C. J., & Gehring, W. J. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.24.9123 · Cited by 770
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector.
Bier, E., Vaessin, H., Shepherd, S., Lee, K., McCall, K., Barbel, S., Ackerman, L., Carretto, R., Uemura, T., & Grell, E. (1989) · Genes & Development · doi:10.1101/gad.3.9.1273 · Cited by 679
P-element-mediated enhancer detection: a versatile method to study development in Drosophila.
Bellen, H. J., O'Kane, C. J., Wilson, C., Grossniklaus, U., Pearson, R. K., & Gehring, W. J. (1989) · Genes & Development · doi:10.1101/gad.3.9.1288 · Cited by 674
A position-effect assay for boundaries of higher order chromosomal domains
Kellum, R., & Schedl, P. (1991) · Cell · doi:10.1016/0092-8674(91)90318-s · Cited by 617
Self-citationA nuclear DNA attachment element mediates elevated and position-independent gene activity
Stief, A., Winter, D. M., Strätling, W. H., & Sippel, A. E. (1989) · Nature · doi:10.1038/341343a0 · Cited by 571
Regulation and function of the Drosophila segmentation gene fushi tarazu
Hiromi, Y., & Gehring, W. J. (1987) · Cell · doi:10.1016/0092-8674(87)90523-x · Cited by 411
Position effects on eukaryotic gene expression
Wilson, C., Bellen, H. J., & Gehring, W. J. (1990) · Annual Review of Cell Biology · doi:10.1146/annurev.cb.06.110190.003335 · Cited by 379
The abdominal region of the bithorax complex
Karch, F. (1985) · Cell · doi:10.1016/0092-8674(85)90014-5 · Cited by 322
Cited by
CTCF mediates methylation-sensitive enhancer-blocking activity at the H19/Igf2 locus
Hark, A. T., Schoenherr, C. J., Katz, D. J., Ingram, R. S., Levorse, J. M., & Tilghman, S. M. (2000) · Nature · doi:10.1038/35013106 · Cited by 1,547
The protein CTCF is required for the enhancer blocking activity of vertebrate insulators
Bell, A. C., West, A. G., & Felsenfeld, G. (1999) · Cell · doi:10.1016/s0092-8674(00)81967-4 · Cited by 1,101
Chung, J. H., Whiteley, M., & Felsenfeld, G. (1993) · Cell · doi:10.1016/0092-8674(93)80052-g · Cited by 854
Going the Distance: A Current View of Enhancer Action
Blackwood, E. M., & Kadonaga, J. T. (1998) · Science · doi:10.1126/science.281.5373.60 · Cited by 836
Renauld, H., Aparicio, O. M., Zierath, P. D., Billington, B. L., Chhablani, S. K., & Gottschling, D. E. (1993) · Genes & Development · doi:10.1101/gad.7.7a.1133 · Cited by 462
Metadata
- Authors
- Kellum, R., & Schedl, P.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1992-05
- Volume
- 12
- Issue
- 5
- Pages
- 2424-2431
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W1893658886
- Cited by
- 398
- References
- 26
- FWCI
- 5.73
- Type
- journal article
- Language
- en
- Open access
- green
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