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Evidence for Involvement of Activin A and Bone Morphogenetic Protein 4 in Mammalian Mesoderm and Hematopoietic Development
Open accessAbstract
Xenopus in vitro studies have implicated both transforming growth factor beta (TGF-beta) and fibroblast growth factor (FGF) families in mesoderm induction. Although members of both families are present during mouse mesoderm formation, there is little evidence for their functional role in mesoderm induction. We show that mouse embryonic stem cells, which resemble primitive ectoderm, can differentiate to mesoderm in vitro in a chemically defined medium (CDM) in the absence of fetal bovine serum. In CDM, this differentiation is responsive to TGF-beta family members in a concentration-dependent manner, with activin A mediating the formation of dorsoanterior-like mesoderm and bone morphogenetic protein 4 mediating the formation of ventral mesoderm, including hematopoietic precursors. These effects are not observed in CDM alone or when TGF-beta 1, -beta 2, or -beta 3, acid FGF, or basic FGF is added individually to CDM. In vivo, at day 6.5 of mouse development, activin beta A RNA is detectable in the decidua and bone morphogenetic protein 4 RNA is detectable in the egg cylinder. Together, our data strongly implicate the TGF-beta family in mammalian mesoderm development and hematopoietic cell formation.
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Cited by
Kattman, S. J., Witty, A. D., Gagliardi, M., Dubois, N. C., Niapour, M., Hotta, A., Ellis, J., & Keller, G. (2011) · Cell Stem Cell · doi:10.1016/j.stem.2010.12.008 · Cited by 1,159
Of lineage and legacy: the development of mammalian hematopoietic stem cells
Dzierzak, E., & Speck, N. A. (2008) · Nature Immunology · doi:10.1038/ni1560 · Cited by 707
Essential function of Gli2 and Gli3 in the formation of lung, trachea and oesophagus
Motoyama, J., Liu, J., Mo, R., Ding, Q., Post, M., & Hui, C.-C. (1998) · Nature Genetics · doi:10.1038/1711 · Cited by 560
Id Genes Are Direct Targets of Bone Morphogenetic Protein Induction in Embryonic Stem Cells
Hollnagel, A., Oehlmann, V., Heymer, J., Rüther, U., & Nordheim, A. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.28.19838 · Cited by 527
Global expression analysis of gene regulatory pathways during endocrine pancreatic development
Gu, G., Wells, J. M., Dombkowski, D., Preffer, F., Aronow, B., & Melton, D. A. (2004) · Development · doi:10.1242/dev.00921 · Cited by 229
Metadata
- Authors
- Johansson, B. M., & Wiles, M. V.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1995-01
- Volume
- 15
- Issue
- 1
- Pages
- 141-151
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2120297737
- Cited by
- 449
- References
- 0
- FWCI
- 7.22
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Evidence for Involvement of Activin A and Bone Morphogenetic Protein 4 in Mammalian Mesoderm and Hematopoietic Development. https://app.cochat.ai/papers/10.1128/mcb.15.1.141
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- Johansson, B. M., & Wiles, M. V. (1995). Evidence for Involvement of Activin A and Bone Morphogenetic Protein 4 in Mammalian Mesoderm and Hematopoietic Development. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.15.1.141
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
