The DOI matches its Crossref record; 79 of the 79 references it cites were found in a registry or an index.
The WRPW Motif of the Hairy-Related Basic Helix-Loop-Helix Repressor Proteins Acts as a 4-Amino-Acid Transcription Repression and Protein-Protein Interaction Domain
Open accessAbstract
Hairy-related proteins include the Drosophila Hairy and Enhancer of Split proteins and mammalian Hes proteins. These proteins are basic helix-loop-helix (bHLH) transcriptional repressors that control cell fate decisions such as neurogenesis or myogenesis in both Drosophila melanogaster and mammals. Hairy-related proteins are site-specific DNA-binding proteins defined by the presence of both a repressor-specific bHLH DNA binding domain and a carboxyl-terminal WRPW (Trp-Arg-Pro-Trp) motif. These proteins act as repressors by binding to DNA sites in target gene promoters and not by interfering with activator proteins, indicating that these proteins are active repressors which should therefore have specific repression domains. Here we show the WRPW motif to be a functional transcriptional repression domain sufficient to confer active repression to Hairy-related proteins or a heterologous DNA-binding protein, Ga14. This motif was previously shown to be necessary for interactions with Groucho, a genetically defined corepressor for Drosophila Hairy-related proteins. Here we show that the WRPW motif is sufficient to recruit Groucho or the TLE mammalian homologs to target gene promoters. We also show that Groucho and TLE proteins actively repress transcription when directly bound to a target gene promoter and identify a novel, highly conserved transcriptional repression domain in these proteins. These results directly demonstrate that Groucho family proteins are active transcriptional corepressors for Hairy-related proteins and are recruited by the 4-amino acid protein-protein interaction domain, WRPW.
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Murre, C., McCaw, P. S., & Baltimore, D. (1989) · Cell · doi:10.1016/0092-8674(89)90682-x · Cited by 2,635
The protein Id: A negative regulator of helix-loop-helix DNA binding proteins
Benezra, R., Davis, R. L., Lockshon, D., Turner, D. L., & Weintraub, H. (1990) · Cell · doi:10.1016/0092-8674(90)90214-y · Cited by 2,238
Hörlein, A. J., Näär, A. M., Heinzel, T., Torchia, J., Gloss, B., Kurokawa, R., Ryan, A., Kamei, Y., Söderström, M., Glass, C. K., et al. (1995) · Nature · doi:10.1038/377397a0 · Cited by 2,034
Domain Interaction Between NMDA Receptor Subunits and the Postsynaptic Density Protein PSD-95
Kornau, H.-C., Schenker, L. T., Kennedy, M. B., & Seeburg, P. H. (1995) · Science · doi:10.1126/science.7569905 · Cited by 1,904
Murre, C., McCaw, P. S., Vaessin, H., Caudy, M., Jan, L. Y., Jan, Y. N., Cabrera, C. V., Buskin, J. N., Hauschka, S. D., Lassar, A. B., et al. (1989) · Cell · doi:10.1016/0092-8674(89)90434-0 · Cited by 1,815
Self-citationA transcriptional co-repressor that interacts with nuclear hormone receptors
Chen, J. D., & Evans, R. M. (1995) · Nature · doi:10.1038/377454a0 · Cited by 1,763
The myoD Gene Family: Nodal Point During Specification of the Muscle Cell Lineage
Weintraub, H., Davis, R., Tapscott, S., Thayer, M., Krause, M., Benezra, R., Blackwell, T. K., Turner, D., Rupp, R., Hollenberg, S., et al. (1991) · Science · doi:10.1126/science.1846704 · Cited by 1,572
Signalling downstream of activated mammalian Notch
Jarriault, S., Brou, C., Logeat, F., Schroeter, E. H., Kopan, R., & Israel, A. (1995) · Nature · doi:10.1038/377355a0 · Cited by 1,390
The MyoD family and myogenesis: Redundancy, networks, and thresholds
Weintraub, H. (1993) · Cell · doi:10.1016/0092-8674(93)90610-3 · Cited by 1,057
Guillemot, F., Lo, L.-C., Johnson, J. E., Auerbach, A., Anderson, D. J., & Joyner, A. L. (1993) · Cell · doi:10.1016/0092-8674(93)90381-y · Cited by 1,033
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Notch Signaling: Cell Fate Control and Signal Integration in Development
Artavanis-Tsakonas, S., Rand, M. D., & Lake, R. J. (1999) · Science · doi:10.1126/science.284.5415.770 · Cited by 5,909
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
Gilbert, L. A., Larson, M. H., Morsut, L., Liu, Z., Brar, G. A., Torres, S. E., Stern-Ginossar, N., Brandman, O., Whitehead, E. H., Doudna, J. A., et al. (2013) · Cell · doi:10.1016/j.cell.2013.06.044 · Cited by 3,696
“We further fused this modified dCas9 gene with different repressive chromatin modifier domains, including the KRAB (Krüppel associated box) domain of Kox1 (Figure 1B), the CS (Chromo Shadow) domain of HP1α, or the WRPW domain of Hes1 (Fisher et al., 1996; Hathaway et al., 2012; Margolin et al., 1994).”
Gridlock signalling pathway fashions the first embryonic artery
Zhong, T. P., Childs, S., Leu, J. P., & Fishman, M. C. (2001) · Nature · doi:10.1038/35102599 · Cited by 553
gridlock , an HLH Gene Required for Assembly of the Aorta in Zebrafish
Zhong, T. P., Rosenberg, M., Mohideen, M.-A. P. K., Weinstein, B., & Fishman, M. C. (2000) · Science · doi:10.1126/science.287.5459.1820 · Cited by 422
Control of the Reversibility of Cellular Quiescence by the Transcriptional Repressor HES1
Sang, L., Coller, H. A., & Roberts, J. M. (2008) · Science · doi:10.1126/science.1155998 · Cited by 313
Metadata
- Authors
- Fisher, A. L., Ohsako, S., & Caudy, M.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1996-06
- Volume
- 16
- Issue
- 6
- Pages
- 2670-2677
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2155186791
- Cited by
- 353
- References
- 79
- FWCI
- 8.53
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: The WRPW Motif of the Hairy-Related Basic Helix-Loop-Helix Repressor Proteins Acts as a 4-Amino-Acid Transcription Repression and Protein-Protein Interaction Domain. https://app.cochat.ai/papers/10.1128/mcb.16.6.2670
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- Fisher, A. L., Ohsako, S., & Caudy, M. (1996). The WRPW Motif of the Hairy-Related Basic Helix-Loop-Helix Repressor Proteins Acts as a 4-Amino-Acid Transcription Repression and Protein-Protein Interaction Domain. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.16.6.2670
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