A Sampling of the Yeast Proteome
Open accessAbstract
In this study, we examined yeast proteins by two-dimensional (2D) gel electrophoresis and gathered quantitative information from about 1,400 spots. We found that there is an enormous range of protein abundance and, for identified spots, a good correlation between protein abundance, mRNA abundance, and codon bias. For each molecule of well-translated mRNA, there were about 4,000 molecules of protein. The relative abundance of proteins was measured in glucose and ethanol media. Protein turnover was examined and found to be insignificant for abundant proteins. Some phosphoproteins were identified. The behavior of proteins in differential centrifugation experiments was examined. Such experiments with 2D gels can give a global view of the yeast proteome.
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Noise in measurements can cause many of the observations attributed to post-transcriptional regulation.
“However, as frequently noted [1, 7, 9, 10, 19–21], noise in measurements can cause many of the observations attributed to post-transcriptional regulation.”
SCM protein levels sum to just over 35 million protein molecules per haploid cell, similar to the 50 million molecules per cell estimated previously within the variation in total protein extraction.
“Scaled to 4μg of protein in 1.5×106 cells (2.7pg protein per yeast cell in cells roughly 30 μm 3 in size) [55], SCM protein levels sum to just over 35 million protein molecules per haploid cell, similar to the 50 million molecules per cell estimated previously [20] within the variation in total protein extraction from haploid yeast cells (cf. [56], which estimates 4.95pg per cell).”
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- Authors
- Futcher, B., Latter, G. I., Monardo, P., McLaughlin, C. S., & Garrels, J. I.
- Affiliations
- Cold Spring Harbor Laboratory (US)
- University of California, Irvine (US)
- Proteome, Inc.
- ORCID
- Futcher, B.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1999-11
- Volume
- 19
- Issue
- 11
- Pages
- 7357-7368
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC84729
- OpenAlex
- W2150566147
- Cited by
- 606
- References
- 1
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 9 · 2023: 9 · 2022: 14 · 2021: 2 · 2020: 8 · 2019: 14 · 2018: 19 · 2017: 14 · 2016: 12 · 2015: 17 · 2014: 21 · 2013: 18 · 2012: 25
- FWCI
- 21.92
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory) (acceptedVersion)
- Funders
- Small Business Innovation Research
- Cold Spring Harbor Laboratory
- National Institutes of Health (2r44gm054110-02, 5r01gm045410-08, 5p41rr002188-10)
- National Institute of General Medical Sciences (R01 GM45410, R44 GM54110)
- National Center for Research Resources (P41 RR02188)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
- Keywords
- yeast proteome, protein abundance, mRNA levels, codon usage bias, protein turnover, phosphoproteins, differential centrifugation, Saccharomyces cerevisiae
- MeSH terms
- Codon, Fungal Proteins, Genes, Fungal, Genetic Code, Image Processing, Computer-Assisted, RNA, Fungal, RNA, Messenger, Saccharomyces cerevisiae, Protein Biosynthesis, Electrophoresis, Gel, Two-Dimensional, Gene Expression Profiling
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