The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Retroviral Expression in Embryonic Stem Cells and Hematopoietic Stem Cells
Open accessAbstract
Achieving long-term retroviral expression in primary cells has been problematic. De novo DNA methylation of infecting proviruses has been proposed as a major cause of this transcriptional repression. Here we report the development of a mouse stem cell virus (MSCV) long terminal repeat-based retroviral vector that is expressed in both embryonic stem (ES) cells and hematopoietic stem (HS) cells. Infected HS cells and their differentiated descendants maintained long-term and stable retroviral expression after serial adoptive transfers. In addition, retrovirally infected ES cells showed detectable expression level of the green fluorescent protein (GFP). Moreover, GFP expression of integrated proviruses was maintained after in vitro differentiation of infected ES cells. Long-term passage of infected ES cells resulted in methylation-mediated silencing, while short-term expression was methylation independent. Tissues of transgenic animals, which we derived from ES cells carrying the MSCV-based provirus, did not express GFP. However, treatment with the demethylating agent 5-azadeoxycytidine reactivated the silent provirus, demonstrating that DNA methylation is involved in the maintenance of retroviral repression. Our results indicate that retroviral expression in ES cells is repressed by methylation-dependent as well as methylation-independent mechanisms.
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Cites
Targeted mutation of the DNA methyltransferase gene results in embryonic lethality
Li, E., Bestor, T. H., & Jaenisch, R. (1992) · Cell · doi:10.1016/0092-8674(92)90611-f · Cited by 3,984
Self-citationTranscription of IAP endogenous retroviruses is constrained by cytosine methylation
Walsh, C. P., Chaillet, J. R., & Bestor, T. H. (1998) · Nature Genetics · doi:10.1038/2413 · Cited by 1,105
De novo DNA cytosine methyltransferase activities in mouse embryonic stem cells
Lei, H., Oh, S. P., Okano, M., Jüttermann, R., Goss, K. A., Jaenisch, R., & Li, E. (1996) · Development · doi:10.1242/dev.122.10.3195 · Cited by 816
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Cited by
The Ink4/Arf locus is a barrier for iPS cell reprogramming
Li, H., Collado, M., Villasante, A., Strati, K., Ortega, S., Cañamero, M., Blasco, M. A., & Serrano, M. (2009) · Nature · doi:10.1038/nature08290 · Cited by 968
Mechanisms of BCR-ABL in the pathogenesis of chronic myelogenous leukaemia
Ren, R. (2005) · Nature Reviews Cancer · doi:10.1038/nrc1567 · Cited by 878
Meenhuis, A., van Veelen, P. A., de Looper, H., van Boxtel, N., van den Berge, I. J., Sun, S. M., Taskesen, E., Stern, P., de Ru, A. H., van Adrichem, A. J., et al. (2011) · Blood · doi:10.1182/blood-2011-02-336487 · Cited by 142
Herbst, F., Ball, C. R., Tuorto, F., Nowrouzi, A., Wang, W., Zavidij, O., Dieter, S. M., Fessler, S., van der Hoeven, F., Kloz, U., et al. (2012) · Molecular Therapy · doi:10.1038/mt.2012.46 · Cited by 104
MECP2 Isoform-Specific Vectors with Regulated Expression for Rett Syndrome Gene Therapy
Rastegar, M., Hotta, A., Pasceri, P., Makarem, M., Cheung, A. Y. L., Elliott, S., Park, K. J., Adachi, M., Jones, F. S., Clarke, I. D., et al. (2009) · PLoS ONE · doi:10.1371/journal.pone.0006810 · Cited by 79
Metadata
- Authors
- Cherry, S. R., Biniszkiewicz, D., van Parijs, L., Baltimore, D., & Jaenisch, R.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2000-10-15
- Volume
- 20
- Issue
- 20
- Pages
- 7419-7426
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2161191369
- Cited by
- 271
- References
- 3
- FWCI
- 9.09
- Type
- journal article
- Language
- en
- Open access
- green
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- Cherry, S. R., Biniszkiewicz, D., van Parijs, L., Baltimore, D., & Jaenisch, R. (2000). Retroviral Expression in Embryonic Stem Cells and Hematopoietic Stem Cells. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.20.20.7419-7426.2000
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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