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Characterization of Schizosaccharomyces pombe Hus1: a PCNA-Related Protein That Associates with Rad1 and Rad9
Open accessAbstract
Hus1 is one of six checkpoint Rad proteins required for all Schizosaccharomyces pombe DNA integrity checkpoints. MYC-tagged Hus1 reveals four discrete forms. The main form, Hus1-B, participates in a protein complex with Rad9 and Rad1, consistent with reports that Rad1-Hus1 immunoprecipitation is dependent on the rad9(+) locus. A small proportion of Hus1-B is intrinsically phosphorylated in undamaged cells and more becomes phosphorylated after irradiation. Hus1-B phosphorylation is not increased in cells blocked in early S phase with hydroxyurea unless exposure is prolonged. The Rad1-Rad9-Hus1-B complex is readily detectable, but upon cofractionation of soluble extracts, the majority of each protein is not present in this complex. Indirect immunofluorescence demonstrates that Hus1 is nuclear and that this localization depends on Rad17. We show that Rad17 defines a distinct protein complex in soluble extracts that is separate from Rad1, Rad9, and Hus1. However, two-hybrid interaction, in vitro association and in vivo overexpression experiments suggest a transient interaction between Rad1 and Rad17.
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[56] Molecular genetic analysis of fission yeast Schizosaccharomyces pombe
Moreno, S., Klar, A., & Nurse, P. (1991) · Methods in Enzymology · doi:10.1016/0076-6879(91)94059-l · Cited by 3,620
Replication Checkpoint Enforced by Kinases Cds1 and Chk1
Boddy, M. N., Furnari, B., Mondesert, O., & Russell, P. (1998) · Science · doi:10.1126/science.280.5365.909 · Cited by 316
Oku, T., Ikeda, S., Sasaki, H., Fukuda, K., Morioka, H., Ohtsuka, E., Yoshikawa, H., & Tsurimoto, T. (1998) · Genes to Cells · doi:10.1046/j.1365-2443.1998.00199.x · Cited by 77
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Norbury, C. J., & Hickson, I. D. (2001) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pharmtox.41.1.367 · Cited by 623
Methylation of Histone H4 Lysine 20 Controls Recruitment of Crb2 to Sites of DNA Damage
Sanders, S. L., Portoso, M., Mata, J., Bähler, J., Allshire, R. C., & Kouzarides, T. (2004) · Cell · doi:10.1016/j.cell.2004.11.009 · Cited by 543
Regulation of ATR substrate selection by Rad17-dependent loading of Rad9 complexes onto chromatin
Zou, L., Cortez, D., & Elledge, S. J. (2002) · Genes & Development · doi:10.1101/gad.950302 · Cited by 498
Regulation of Chromosome Replication
Kelly, T. J., & Brown, G. W. (2000) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.69.1.829 · Cited by 401
A DNA Damage Response Pathway Controlled by Tel1 and the Mre11 Complex
Usui, T., Ogawa, H., & Petrini, J. H. J. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00270-2 · Cited by 384
Metadata
- Authors
- Caspari, T., Dahlen, M., Kanter-Smoler, G., Lindsay, H. D., Hofmann, K., Papadimitriou, K., Sunnerhagen, P., & Carr, A. M.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2000-02-01
- Volume
- 20
- Issue
- 4
- Pages
- 1254-1262
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2109482063
- Cited by
- 231
- References
- 3
- FWCI
- 10.59
- Type
- journal article
- Language
- en
- Open access
- green
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- Caspari, T., Dahlen, M., Kanter-Smoler, G., Lindsay, H. D., Hofmann, K., Papadimitriou, K., Sunnerhagen, P., & Carr, A. M. (2000). Characterization of Schizosaccharomyces pombe Hus1: a PCNA-Related Protein That Associates with Rad1 and Rad9. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.20.4.1254-1262.2000
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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