The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Spatial and Temporal Cellular Responses to Single-Strand Breaks in Human Cells
Open accessAbstract
DNA single-strand breaks (SSB) are one of the most frequent DNA lesions produced by reactive oxygen species and during DNA metabolism, but the analysis of cellular responses to SSB remains difficult due to the lack of an experimental method to produce SSB alone in cells. By using human cells expressing a foreign UV damage endonuclease (UVDE) and irradiating the cells with UV through tiny pores in membrane filters, we created SSB in restricted areas in the nucleus by the immediate action of UVDE on UV-induced DNA lesions. Cellular responses to the SSB were characterized by using antibodies and fluorescence microscopy. Upon UV irradiation, poly(ADP-ribose) synthesis occurred immediately in the irradiated area. Simultaneously, but dependent on poly(ADP-ribosyl)ation, XRCC1 was translocated from throughout the nucleus, including nucleoli, to the SSB. The BRCT1 domain of XRCC1 protein was indispensable for its poly(ADP-ribose)-dependent recruitment to the SSB. Proliferating cell nuclear antigen and the p150 subunit of chromatin assembly factor 1 also accumulated at the SSB in a detergent-resistant form, which was significantly reduced by inhibition of poly(ADP-ribose) synthesis. Our results show the importance of poly(ADP-ribosyl)ation in sequential cellular responses to SSB.
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Katsumi, S., Kobayashi, N., Imoto, K., Nakagawa, A., Yamashina, Y., Muramatsu, T., Shirai, T., Miyagawa, S., Sugiura, S., Hanaoka, F., et al. (2001) · Journal of Investigative Dermatology · doi:10.1046/j.0022-202x.2001.01540.x · Cited by 119
Self-citationTakao, M., Yonemasu, R., Yamamoto, K., & Yasui, A. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.7.1267 · Cited by 72
Self-citationOkano, S., Kanno, S.-I., Nakajima, S., & Yasui, A. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m004085200 · Cited by 47 · cited 8×
“This is consistent with our previous result showing that after wholecell UV irradiation with 20 J/m(2), repair synthesis was decreased to about 30% by aphidicoline, suggesting polymerase / -dependent long-patch repair for UVDE-induced SSB (27).”
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Cited by
Poly(ADP-ribose): novel functions for an old molecule
Schreiber, V., Dantzer, F., Ame, J.-C., & de Murcia, G. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1963 · Cited by 1,915
Advances in autism genetics: on the threshold of a new neurobiology
Abrahams, B. S., & Geschwind, D. H. (2008) · Nature Reviews Genetics · doi:10.1038/nrg2346 · Cited by 1,866
New insights into the molecular and cellular functions of poly(ADP-ribose) and PARPs
Gibson, B. A., & Kraus, W. L. (2012) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3376 · Cited by 1,226
Dynamics of DNA damage response proteins at DNA breaks: a focus on protein modifications
Polo, S. E., & Jackson, S. P. (2011) · Genes & Development · doi:10.1101/gad.2021311 · Cited by 1,092
The PARP Side of the Nucleus: Molecular Actions, Physiological Outcomes, and Clinical Targets
Krishnakumar, R., & Kraus, W. L. (2010) · Molecular Cell · doi:10.1016/j.molcel.2010.06.017 · Cited by 836
Metadata
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2003-08-01
- Volume
- 23
- Issue
- 15
- Pages
- 5472-5472
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2102207473
- Cited by
- 297
- References
- 3
- FWCI
- 8.15
- Type
- journal article
- Language
- en
- Open access
- bronze
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