Abl Interactor 1 (Abi-1) Wave-Binding and SNARE Domains Regulate Its Nucleocytoplasmic Shuttling, Lamellipodium Localization, and Wave-1 Levels
Open accessAbstract
The Abl interactor 1 (Abi-1) protein has been implicated in the regulation of actin dynamics and localizes to the tips of lamellipodia and filopodia. Here, we show that Abi-1 binds the actin nucleator protein Wave-1 through an amino-terminal Wave-binding (WAB) domain and that disruption of the Abi-1-Wave-1 interaction prevents Abi-1 from reaching the tip of the lamellipodium. Abi-1 binds to the Wave homology domain of Wave-1, a region that is required for translocation of Wave-1 to the lamellipodium. Mouse embryo fibroblasts that lack one allele of Abi-1 and are homozygous null for the related Abi-2 protein exhibit decreased Wave-1 protein levels. This phenotype is rescued by Abi-1 proteins that retain Wave-1 binding but not by Abi-1 mutants that cannot bind to Wave-1. Moreover, we uncovered an overlapping SNARE domain in the amino terminus of Abi-1 that interacts with Syntaxin-1, a SNARE family member. Further, we demonstrated that Abi-1 shuttles in and out of the nucleus in a leptomycin B (LMB)-dependent manner and that complete nuclear translocation of Abi-1 in the absence of LMB requires the combined inactivation of the SNARE, WAB, and SH3 domains of Abi-1. Thus, Abi-1 undergoes nucleocytoplasmic shuttling and functions at the leading edge to regulate Wave-1 localization and protein levels.
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Treatment of cells with leptomycin B (an inhibitor of the nuclear exporter CRM-1) induces the nuclear accumulation of ABI1 and CYFIP2.
“Treatment of cells with leptomycin B (an inhibitor of the nuclear exporter CRM-1) induces the nuclear accumulation of ABI184 and CYFIP285.”
From The WAVE complex in developmental and adulthood brain disorders (2025) · cites this paper as [84]
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Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 Å resolution
Sutton, R. B., Fasshauer, D., Jahn, R., & Brunger, A. T. (1998) · Nature · doi:10.1038/26412 · Cited by 2,417 · cited 20× · see citing works
“The Q-SNARE motif of Syntaxin and SNAP-25 family proteins contains conserved layers of interacting amino acid side chains.”
BackgroundInfluentialTakenawa, T., & Miki, H. (2001) · Journal of Cell Science · doi:10.1242/jcs.114.10.1801 · Cited by 604 · see citing works
Kunda, P., Craig, G., Dominguez, V., & Baum, B. (2003) · Current Biology · doi:10.1016/j.cub.2003.10.005 · Cited by 341 · see citing works
Chapman, E. R., An, S., Barton, N., & Jahn, R. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47003-2 · Cited by 295 · see citing works
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Research works printed but not linked: 4
- [17]17. Fasshauer, D., R. B. Sutton, A. T. Brunger, and R. Jahn. 1998. Conserved structural features of the synaptic fusion complex: SNARE proteins reclassified as Q- and R-SNAREs. Proc. Natl. Acad. Sci. USA 95: 15781-15786.
- [26]26. Machesky, L. M., R. D. Mullins, H. N. Higgs, D. A. Kaiser, L. Blanchoin, R. C. May, M. E. Hall, and T. D. Pollard. 1999. Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complex. Proc. Natl. Acad. Sci. USA 96: 3739-3744.
- [44]44. Soderling, S. H., L. K. Langeberg, J. A. Soderling, S. M. Davee, R. Simerly, J. Raber, and J. D. Scott. 2003. Loss of WAVE-1 causes sensorimotor retardation and reduced learning and memory in mice. Proc. Natl. Acad. Sci. USA 100: 1723-1728.
- [51]51. Taagepera, S., D. McDonald, J. E. Loeb, L. L. Whitaker, A. K. McElroy, J. Y. Wang, and T. J. Hope. 1998. Nuclear-cytoplasmic shuttling of C-ABL tyrosine kinase. Proc. Natl. Acad. Sci. USA 95: 7457-7462.
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SNAREs — engines for membrane fusion
Jahn, R., & Scheller, R. H. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2002 · Cited by 2,635 · see citing works
Grove, M., Demyanenko, G., Echarri, A., Zipfel, P. A., Quiroz, M. E., Rodriguiz, R. M., Playford, M., Martensen, S. A., Robinson, M. R., Wetsel, W. C., et al. (2004) · Molecular and Cellular Biology · doi:10.1128/mcb.24.24.10905-10922.2004 · Cited by 136 · see citing works
Self-citationRakeman, A. S., & Anderson, K. V. (2006) · Development · doi:10.1242/dev.02473 · Cited by 107 · see citing works
The WAVE complex in developmental and adulthood brain disorders
Kim, H.-G., Berdasco, C., Nairn, A. C., & Kim, Y. (2025) · Experimental & Molecular Medicine · doi:10.1038/s12276-024-01386-w · Cited by 8 · see citing works
- Authors
- Echarri, A., Lai, M. J., Robinson, M. R., & Pendergast, A. M.
- Affiliations
- Department of Pharmacology and Cancer Biology
- Duke University (US)
- Corresponding author
- Pendergast, Ann Marie
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2004-06-01
- Volume
- 24
- Issue
- 11
- Pages
- 4979-4993
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC416433
- OpenAlex
- W2171589330
- Cited by
- 88
- References
- 4
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 2 · 2014: 3 · 2013: 2 · 2012: 10
- FWCI
- 3.07
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Cancer Institute (R01CA070940, CA70940)
- National Institute of General Medical Sciences (R01GM062375, GM 62375)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- Abi-1, syntaxin-1, nucleocytoplasmic shuttling, lamellipodia, actin dynamics, actin nucleation, leptomycin B, SH3 domain, cell motility, mouse embryonic fibroblasts
- MeSH terms
- Amino Acid Sequence, Animals, Antigens, Surface, Cytoskeletal Proteins, Membrane Proteins, Microfilament Proteins, Molecular Sequence Data, Nerve Tissue Proteins, Protein Structure, Tertiary, Vesicular Transport Proteins, NIH 3T3 Cells, Adaptor Proteins, Signal Transducing, SNARE Proteins, Syntaxin 1, Wiskott-Aldrich Syndrome Protein Family, Mice
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