The DOI matches its Crossref record; 56 of the 56 references it cites were found in a registry or an index.
mTOR Is Essential for Growth and Proliferation in Early Mouse Embryos and Embryonic Stem Cells
Open accessAbstract
TOR is a serine-threonine kinase that was originally identified as a target of rapamycin in Saccharomyces cerevisiae and then found to be highly conserved among eukaryotes. In Drosophila melanogaster, inactivation of TOR or its substrate, S6 kinase, results in reduced cell size and embryonic lethality, indicating a critical role for the TOR pathway in cell growth control. However, the in vivo functions of mammalian TOR (mTOR) remain unclear. In this study, we disrupted the kinase domain of mouse mTOR by homologous recombination. While heterozygous mutant mice were normal and fertile, homozygous mutant embryos died shortly after implantation due to impaired cell proliferation in both embryonic and extraembryonic compartments. Homozygous blastocysts looked normal, but their inner cell mass and trophoblast failed to proliferate in vitro. Deletion of the C-terminal six amino acids of mTOR, which are essential for kinase activity, resulted in reduced cell size and proliferation arrest in embryonic stem cells. These data show that mTOR controls both cell size and proliferation in early mouse embryos and embryonic stem cells.
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Self-citationPten is essential for embryonic development and tumour suppression
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Cited by
mTOR Signaling in Growth Control and Disease
Laplante, M., & Sabatini, D. M. (2012) · Cell · doi:10.1016/j.cell.2012.03.017 · Cited by 8,509
TOR signaling in growth and metabolism
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mTOR: from growth signal integration to cancer, diabetes and ageing
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Defining the role of mTOR in cancer
Guertin, D. A., & Sabatini, D. M. (2007) · Cancer Cell · doi:10.1016/j.ccr.2007.05.008 · Cited by 2,833
mTOR and cancer: insights into a complex relationship
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Metadata
- Authors
- Murakami, M., Ichisaka, T., Maeda, M., Oshiro, N., Hara, K., Edenhofer, F., Kiyama, H., Yonezawa, K., & Yamanaka, S.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2004-08
- Volume
- 24
- Issue
- 15
- Pages
- 6710-6718
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2141424872
- Cited by
- 624
- References
- 56
- FWCI
- 7.54
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: mTOR Is Essential for Growth and Proliferation in Early Mouse Embryos and Embryonic Stem Cells. https://app.cochat.ai/papers/10.1128/mcb.24.15.6710-6718.2004
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- Murakami, M., Ichisaka, T., Maeda, M., Oshiro, N., Hara, K., Edenhofer, F., Kiyama, H., Yonezawa, K., & Yamanaka, S. (2004). mTOR Is Essential for Growth and Proliferation in Early Mouse Embryos and Embryonic Stem Cells. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.24.15.6710-6718.2004
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