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Cited by 26Bacterial Genetics and BiotechnologyPhosphodiesterase function and regulationBiochemical and Molecular Research

Bacterial cell volume regulation and the importance of cyclic di-AMP

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Foster, Alexander J., van den Noort, Marco, Poolman, Bert·2024·Microbiology and Molecular Biology Reviews
DOI 10.1128/mmbr.00181-23PMC PMC11332354
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Abstract

SUMMARY: Nucleotide-derived second messengers are present in all domains of life. In prokaryotes, most of their functionality is associated with general lifestyle and metabolic adaptations, often in response to environmental fluctuations of physical parameters. In the last two decades, cyclic di-AMP has emerged as an important signaling nucleotide in many prokaryotic lineages, including Firmicutes, Actinobacteria, and Cyanobacteria. Its importance is highlighted by the fact that both the lack and overproduction of cyclic di-AMP affect viability of prokaryotes that utilize cyclic di-AMP, and that it generates a strong innate immune response in eukaryotes. In bacteria that produce the second messenger, most molecular targets of cyclic di-AMP are associated with cell volume control. Besides, other evidence links the second messenger to cell wall remodeling, DNA damage repair, sporulation, central metabolism, and the regulation of glycogen turnover. In this review, we take a biochemical, quantitative approach to address the main cellular processes that are directly regulated by cyclic di-AMP and show that these processes are very connected and require regulation of a similar set of proteins to which cyclic di-AMP binds. Altogether, we argue that cyclic di-AMP is a master regulator of cell volume and that other cellular processes can be connected with cyclic di-AMP through this core function. We further highlight important directions in which the cyclic di-AMP field has to develop to gain a full understanding of the cyclic di-AMP signaling network and why some processes are regulated, while others are not.

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  • K+ maintains osmotic balance and is an important contributor to the membrane potential.

    “This dominant cation maintains osmotic balance7,46,47 and is an important contributor to the membrane potential.48”

    From Metals in Motion: Understanding Labile Metal Pools in Bacteria (2025) · cites this paper as [46]

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Authors
Foster, A. J., van den Noort, M., & Poolman, B.
Affiliations
  • University of Groningen (NL)
  • Department of Biochemistry, Groningen Biomolecular Science and Biotechnology Institute, University of Groningen, Groningen, the Netherlands
Corresponding author
Poolman, Bert
ORCID
Venue
Microbiology and Molecular Biology Reviews
Publisher
American Society for Microbiology
Published
2024-06-27
Volume
88
Issue
2
Pages
e0018123-e0018123
ISSN
  • 1092-2172 (ISSN-L)
  • 1098-5557
DOI
10.1128/mmbr.00181-23
PMC
PMC11332354
OpenAlex
W4399495726
Cited by
26
References
4
Citations by year
2026: 14 · 2025: 12
FWCI
1.67
Type
journal article
Language
en
Open access
Licence
Locations
  • Microbiology and Molecular Biology Reviews (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion, other-oa)
Funders
NWO (BaSyC Gravitation program, OCENW.KLEIN.526)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phosphodiesterase function and regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
Keywords
cyclic di-AMP, nucleotide second messengers, cell wall remodeling, DNA repair, sporulation, glycogen turnover, innate immune response, Firmicutes, actinobacteria, cyanobacteria
MeSH terms
Bacteria, Bacterial Proteins, Cell Wall, Dinucleoside Phosphates, Second Messenger Systems, Signal Transduction, Gene Expression Regulation, Bacterial

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