The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Protein-protein interactions: methods for detection and analysis.
Open accessAbstract
The function and activity of a protein are often modulated by other proteins with which it interacts. This review is intended as a practical guide to the analysis of such protein-protein interactions. We discuss biochemical methods such as protein affinity chromatography, affinity blotting, coimmunoprecipitation, and cross-linking; molecular biological methods such as protein probing, the two-hybrid system, and phage display: and genetic methods such as the isolation of extragenic suppressors, synthetic mutants, and unlinked noncomplementing mutants. We next describe how binding affinities can be evaluated by techniques including protein affinity chromatography, sedimentation, gel filtration, fluorescence methods, solid-phase sampling of equilibrium solutions, and surface plasmon resonance. Finally, three examples of well-characterized domains involved in multiple protein-protein interactions are examined. The emphasis of the discussion is on variations in the approaches, concerns in evaluating the results, and advantages and disadvantages of the techniques.
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Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
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Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
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Protein-Protein Interaction Detection: Methods and Analysis
Rao, V. S., Srinivas, K., Sujini, G. N., & Kumar, G. N. S. (2014) · International Journal of Proteomics · doi:10.1155/2014/147648 · Cited by 811
Protein interaction maps for complete genomes based on gene fusion events
Enright, A. J., Iliopoulos, I., Kyrpides, N. C., & Ouzounis, C. A. (1999) · Nature · doi:10.1038/47056 · Cited by 818
Integrative approaches for finding modular structure in biological networks
Mitra, K., Carvunis, A.-R., Ramesh, S. K., & Ideker, T. (2013) · Nature Reviews Genetics · doi:10.1038/nrg3552 · Cited by 535
Metadata
- Authors
- Phizicky, E. M., & Fields, S.
- Venue
- Microbiological reviews
- Publisher
- American Society for Microbiology
- Published
- 1995
- Volume
- 59
- Issue
- 1
- Pages
- 94-123
- ISSN
- 0146-0749 (ISSN-L)
- OpenAlex
- W2130832525
- Cited by
- 966
- References
- 6
- FWCI
- 18.66
- Type
- journal article
- Language
- en
- Open access
- diamond
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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