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Cited by 109Bacterial biofilms and quorum sensingBurn Injury Management and OutcomesInfections and bacterial resistance

Effect of Human Burn Wound Exudate on Pseudomonas aeruginosa Virulence

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Gonzalez, Manuel R., Fleuchot, Betty, Lauciello, Leonardo, Jafari, Paris, Applegate, Lee Ann, Raffoul, Wassim, Que, Yok-Ai, Perron, Karl·2016·mSphere
DOI 10.1128/msphere.00111-15PMC PMC4894682
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Abstract

Microbial infection of severe burn wounds is currently a major medical challenge. Of the infections by bacteria able to colonize such injuries, those by Pseudomonas aeruginosa are among the most severe, causing major delays in burn patient recovery or leading to fatal issues. In this study, we investigated the growth properties of several burn wound pathogens in biological fluids secreted from human burn wounds. We found that P. aeruginosa strains were able to proliferate but not those of the other pathogens tested. In addition, burn wound exudates (BWEs) stimulate the expression of virulence factors in P. aeruginosa . The chemical composition analysis of BWEs enabled us to determine the major components of these fluids. These data are essential for the development of an artificial medium mimicking the burn wound environment and for in vitro analysis of the initial step in the development of burn wound infections.

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  • A higher GLN requirement in burn patients is explained by exudative losses: analysis of burn exudates shows that GLN is lost in larger amounts than any other amino acid.

    “A higher GLN requirement in burn patients is explained by exudative losses: analysis of burn exudates shows that GLN is lost in larger amounts than any other amino acid [80].”

    From ESPEN practical and partially revised guideline: Clinical nutrition in the intensive care unit (2023) · cites this paper as [80]

    The Results (Physicochemical characterization of burn wound exudate; page not provided) say glutamine was the only amino acid at a reduced concentration in both exudate and patient serum relative to clinical reference values. The Discussion links low glutamine in exudate to depletion in burn patients. Neither passage establishes that more glutamine is lost through exudate than any other amino acid or measures the resulting increase in glutamine requirement. (LLM, conf=0.86)

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  • The most recent randomized trial published in 2014 confirmed the reduction of infectious complications in 60 patients.

    “The most recent randomized trial was published in 2014 ( 217) confirmed the reduction of infectious complications in 60 patients.”

    From ESPEN guideline on clinical nutrition in the intensive care unit. (2019) · cites this paper as [217]

    The Materials and Methods section describes collecting burn-wound exudate samples for bacterial experiments, not a randomized clinical trial. Searches for a 2014 trial, 60 patients, and reduced infectious complications found no passage in our copy reporting that claim. (LLM, conf=0.76)

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Cited by

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Authors
Gonzalez, M. R., Fleuchot, B., Lauciello, L., Jafari, P., Applegate, L. A., Raffoul, W., Que, Y.-A., & Perron, K.
Affiliations
  • University of Geneva (CH)
  • Centre Hospitalier Universitaire Vaudois (CH)
Corresponding author
Perron, Karl
ORCID
Venue
mSphere
Publisher
American Society for Microbiology
Published
2016-04-27
Volume
1
Issue
2
ISSN
2379-5042 (ISSN-L)
DOI
10.1128/msphere.00111-15
PMC
PMC4894682
OpenAlex
W2342611068
Cited by
109
References
1
Citations by year
2026: 7 · 2025: 8 · 2024: 19 · 2023: 11 · 2022: 15 · 2021: 15 · 2020: 10 · 2019: 8 · 2018: 8 · 2017: 4
FWCI
3.47
Type
journal article
Language
en
Open access
Licence
Locations
  • mSphere (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Archive ouverte UNIGE (University of Geneva) (publishedVersion, other-oa)
  • IRIS (submittedVersion)
  • Bern Open Repository and Information System (University of Bern) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • SERVAL (Université de Lausanne) (submittedVersion, other-oa)
  • Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
  • Open Access CRIS of the University of Bern
Funders
SwissTransMed CRUS (14/2013)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Health Sciences › Medicine › Epidemiology › Burn Injury Management and Outcomes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Infections and bacterial resistance
Keywords
Pseudomonas aeruginosa, bacterial virulence, burn sepsis, Acinetobacter baumannii, Staphylococcus aureus, pyocyanin, pyoverdine, virulence factor expression, burn wound infection

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