In Vitro Gut Modeling as a Tool for Adaptive Evolutionary Engineering of Lactiplantibacillus plantarum
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Improvement of bacterial strains in regard to specific abiotic environmental factors is broadly used to enhance strain characteristics for processing and product quality. However, there is currently no multidimensional probiotic strain improvement approach for both abiotic and biotic factors of a colon microbiota.
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This new junction was not identified in transcriptome samples from the wild type, IA01t4, nor in samples of a previously constructed non-aggregating lamC knockout strain L. plantarum ΔLP_RS14990.
“This new junction was not identified in the transcriptome samples from the wild type, IA01t4, nor in the samples of a previously constructed non-aggregating lamC knockout strain L. plantarum ΔLP_RS14990 [40] (Isenring, unpublished results).”
From Bistable auto-aggregation phenotype in Lactiplantibacillus plantarum emerges after cultivation in in vitro colonic microbiota (2021) · cites this paper as [40]
The Materials and Methods section (“Plasmid construction and LP_RS14990 gene replacement”; page unavailable) confirms that the authors constructed an L. plantarum ΔLP_RS14990 knockout strain. The passages do not identify LP_RS14990 as lamC or establish that the knockout is non-aggregating. The transcriptome-junction finding is separately attributed to unpublished results, not to this citation. (LLM, conf=0.84)
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Isenring, J., Geirnaert, A., Lacroix, C., & Stevens, M. J. A. (2021) · BMC Microbiology · doi:10.1186/s12866-021-02331-x · Cited by 42 · see citing works
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- Authors
- Isenring, J., Geirnaert, A., Hall, A. R., Jans, C., Lacroix, C., & Stevens, M. J. A.
- Affiliations
- ETH Zurich (CH)
- University of Zurich (CH)
- Corresponding author
- Lacroix, Christophe
- Venue
- mSystems
- Publisher
- American Society for Microbiology
- Published
- 2021-04-27
- Volume
- 6
- Issue
- 2
- ISSN
- 2379-5077 (ISSN-L)
- PMC
- PMC8546992
- OpenAlex
- W3155472318
- Cited by
- 17
- References
- 65
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 3 · 2023: 5 · 2022: 2 · 2021: 2
- FWCI
- 1.79
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- mSystems (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nd)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Repository for Publications and Research Data (ETH Zurich) (publishedVersion, cc-by)
- Zurich Open Repository and Archive (University of Zurich) (acceptedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Zurich Open Repository and Archive (University of Zurich) (submittedVersion, cc-by)
- ETH Zürich Research Collection (cc-by)
- Zurich Open Repository and Archive (University of Zurich)
- Funders
- ETH Zürich Foundation (ETH-42 16-1)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Lactobacillus plantarum, in vitro gut model, colonic microbiota, abiotic factors, biotic factors, strain improvement
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