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Bifidobacterium strains from resident infant human gastrointestinal microflora exert antimicrobial activity
Open accessAbstract
BACKGROUND AND AIMS: The gastrointestinal microflora exerts a barrier effect against enteropathogens. The aim of this study was to examine if bifidobacteria, a major species of the human colonic microflora, participates in the barrier effect by developing antimicrobial activity against enterovirulent bacteria. METHODS: Antibacterial activity was examined in vitro against a wide range of Gram negative and Gram positive pathogens. Inhibition of Salmonella typhimurium SL1334 cell association and cell invasion was investigated in vitro using Caco-2 cells. Colonisation of the gastrointestinal tract in vivo by bifidobacteria was examined in axenic C3/He/Oujco mice. Antimicrobial activity was examined in vivo in axenic C3/He/Oujco mice infected by the lethal S typhimurium C5 strain. RESULTS: Fourteen human bifidobacterium strains isolated from infant stools were examined for antimicrobial activity. Two strains (CA1 and F9) expressed antagonistic activity against pathogens in vitro, inhibited cell entry, and killed intracellular S typhimurium SL1344 in Caco-2 cells. An antibacterial component(s) produced by CA1 and F9 was found to be a lipophilic molecule(s) with a molecular weight of less than 3500. In the axenic C3/He/Oujco mice, CA1 and F9 strains colonised the intestinal tract and protected mice against S typhimurium C5 lethal infection. CONCLUSION: Several bifidobacterium strains from resident infant human gastrointestinal microflora exert antimicrobial activity, suggesting that they could participate in the "barrier effect" produced by the indigenous microflora.
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“these bacteria have been linked to fortification of the gut mucosal protection through activities against pathogens12, 13.”
From Diet and the Intestinal Microbiome: Associations, Functions, and Implications for Health and Disease (2014) · cites this paper as [12]
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“As bacteria compete for nutrients and produce substances that directly affect the growth of other bacteria, probiotics can provide a twofold protection against a broad range of pathogens, including certain forms of Clostridium, Escherichia coli, Salmonella, Shigella and Pseudomonas”
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Metadata
- Authors
- Liévin, V, Peiffer, I., Hudault, S., Rochat, F., Brassart, D., Neeser, J.-R., & Servin, A. L.
- Affiliations
- aInstitut National de la Santé et de la Recherche Médicale, Unité 510, UFR de Pharmacie, Université Paris XI, 92296 Châtenay-Malabry, France, bNestlé Research Centre, Vers-chez-les-blanc, BP 44, CH-1000 Lausanne 26, Switzerland
- Inserm (FR)
- Corresponding author
- Servin, A L
- Venue
- Gut
- Publisher
- BMJ
- Published
- 2000-11-01
- Volume
- 47
- Issue
- 5
- Pages
- 646-652
- ISSN
- 0017-5749 (ISSN-L)
- 1468-3288
- PMC
- PMC1728100
- OpenAlex
- W2110840202
- Cited by
- 397
- References
- 54
- Citations by year
- 2026: 10 · 2025: 5 · 2024: 18 · 2023: 17 · 2022: 14 · 2021: 20 · 2020: 14 · 2019: 16 · 2018: 17 · 2017: 11 · 2016: 24 · 2015: 19 · 2014: 23 · 2013: 18 · 2012: 16
- FWCI
- 12.76
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Gut (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- HighWire Press Open Archive (submittedVersion)
- Funders
- Institut National de la Santé et de la Recherche Médicale
- Nestec
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- Bifidobacterium, antimicrobial activity, infant gut microbiota, Salmonella typhimurium, cell invasion inhibition, Caco-2 cells, intestinal colonization, germ-free mice, probiotic bacteria
- MeSH terms
- Animals, Bacteriolysis, Bifidobacterium, Escherichia coli, Feces, Humans, Infant, Klebsiella pneumoniae, Listeria monocytogenes, Mice, Inbred C3H, Pseudomonas aeruginosa, Salmonella typhimurium, Shigella flexneri, Staphylococcus aureus, Streptococcus, Clostridioides difficile, Mice
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