Acute restraint stress activates functional NK1 receptor in the colon of female rats: involvement of steroids
Open accessAbstract
Background and aims: Psychological factors have been implicated in the aetiology of irritable bowel syndrome characterised by intestinal altered motility and visceral hypersensitivity. Similar disorders have been found in rats under stressful conditions. The role of tachykinins in bowel dysfunctions caused by stress is not fully documented. Therefore, we investigated the influence of stress on NK1 receptor activation at the colonic level in female rats. Methods: The stress procedure used consisted of two hours of partial restraint. Histamine release was measured from colonic samples of control and stressed animals and the effect of SR140333, a NK1 receptor antagonist, on substance P induced histamine release was determined. Involvement of steroids has been evaluated in this response. Results: NK1 receptor antagonist was found to inhibit substance P induced histamine release in samples from stressed female rats but not in samples from control animals. Previous treatment of female rats with RU 486 abolished this effect observed in stressed animals. Similarly, in samples from stressed female rats previously ovariectomised, SR140333 failed to inhibit substance P induced histamine release but previous treatment with both progesterone and oestrogen restored its effect. Conclusions: Stress induces NK1 receptor activation in the colon, and ovarian steroids are involved in this response.
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Increased local production of estrogen and proinflammatory cytokines may induce NK1R in myometrium, causing uterine hyperactivity and subsequently pain.
“This may suggest that increased local production of estrogen and proinflammatory cytokines may induce NK1R in myometrium, causing uterine hyperactivity and, subsequently, pain, as in colon72,73.”
From Neuropeptides Substance P and Calcitonin Gene Related Peptide Accelerate the Development and Fibrogenesis of Endometriosis (2019) · cites this paper as [72]
NK1R antagonism reduces anxiety and emotional arousal circuit response to noxious visceral distension in women with irritable bowel syndrome.
“Moreover, NK1R antagonism has been shown to reduce anxiety and emotional arousal circuit response to noxious visceral distension in women with irritable bowel syndrome107, which is likely attributable, at least in part, to increased colonic hypermotility induced by SP and NK1R activation72,73.”
From Neuropeptides Substance P and Calcitonin Gene Related Peptide Accelerate the Development and Fibrogenesis of Endometriosis (2019) · cites this paper as [72]
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Self-citation
- Authors
- Bradesi, S., Eutamene, H., Fioramonti, J., & Bueno, L.
- Affiliations
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Ecole Supérieure d'Agriculture de Purpan, 31076 Toulouse Cedex, France
- Corresponding author
- Bueno, L
- Venue
- Gut
- Publisher
- BMJ
- Published
- 2002-03
- Volume
- 50
- Issue
- 3
- Pages
- 349-354
- ISSN
- 0017-5749 (ISSN-L)
- 1468-3288
- PMC
- PMC1773147
- OpenAlex
- W1983354885
- Cited by
- 34
- References
- 53
- Citations by year
- 2026: 1 · 2025: 1 · 2022: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2015: 1 · 2014: 2
- FWCI
- 2.52
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Gut (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- HighWire Press Open Archive (submittedVersion)
- Topics
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
- Keywords
- acute restraint stress, neurokinin-1 receptor, substance P, histamine release, colonic mucosa, irritable bowel syndrome, tachykinins, ovarian steroids, progesterone, estrogen, ovariectomy, SR140333, female rats
- MeSH terms
- Animals, Colon, Female, Histamine Release, Hormone Antagonists, Ovary, Peptide Fragments, Piperidines, Quinuclidines, Restraint, Physical, Gonadal Steroid Hormones, Stress, Psychological, Substance P, Mifepristone, Rats, Wistar, Receptors, Neurokinin-1, Rats, Neurokinin-1 Receptor Antagonists
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