The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Chronic electronic cigarette exposure in mice induces features of COPD in a nicotine-dependent manner
Open accessAbstract
BACKGROUND: The use of electronic (e)-cigarettes is increasing rapidly, but their lung health effects are not established. Clinical studies examining the potential long-term impact of e-cigarette use on lung health will take decades. To address this gap in knowledge, this study investigated the effects of exposure to aerosolised nicotine-free and nicotine-containing e-cigarette fluid on mouse lungs and normal human airway epithelial cells. METHODS: Mice were exposed to aerosolised phosphate-buffered saline, nicotine-free or nicotine-containing e-cigarette solution, 1-hour daily for 4 months. Normal human bronchial epithelial (NHBE) cells cultured at an air-liquid interface were exposed to e-cigarette vapours or nicotine solutions using a Vitrocell smoke exposure robot. RESULTS: Inhalation of nicotine-containing e-cigarettes increased airway hyper-reactivity, distal airspace enlargement, mucin production, cytokine and protease expression. Exposure to nicotine-free e-cigarettes did not affect these lung parameters. NHBE cells exposed to nicotine-containing e-cigarette vapour showed impaired ciliary beat frequency, airway surface liquid volume, cystic fibrosis transmembrane regulator and ATP-stimulated K+ ion conductance and decreased expression of FOXJ1 and KCNMA1. Exposure of NHBE cells to nicotine for 5 days increased interleukin (IL)-6 and IL-8 secretion. CONCLUSIONS: Exposure to inhaled nicotine-containing e-cigarette fluids triggered effects normally associated with the development of COPD including cytokine expression, airway hyper-reactivity and lung tissue destruction. These effects were nicotine-dependent both in the mouse lung and in human airway cells, suggesting that inhaled nicotine contributes to airway and lung disease in addition to its addictive properties. Thus, these findings highlight the potential dangers of nicotine inhalation during e-cigarette use.
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Short-term studies of electronic cigarette aerosols in adult mice show pulmonary and behavioral effects resembling those from conventional nicotine.
“shorter-term studies of exposures to electronic cigarette-derived aerosols in adult mouse models demonstrate that electronic cigarettes can produce pulmonary and behavioral effects similar to those seen with conventional nicotine exposures.”
From Developmental toxicity of nicotine: A transdisciplinary synthesis and implications for emerging tobacco products (2017) · cites this paper as [26]
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Cited by
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What are the respiratory effects of e-cigarettes?
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Metadata
- Authors
- Garcia-Arcos, I., Geraghty, P., Baumlin, N., Campos, M., Dabo, A. J., Jundi, B., Cummins, N., Eden, E., Grosche, A., Salathe, M., & Foronjy, R.
- Affiliations
- SUNY Downstate Health Sciences University (US)
- University of Miami (US)
- Royal College of Surgeons in Ireland (IE)
- Mount Sinai Health System (US)
- Corresponding author
- Foronjy, Robert
- ORCID
- Venue
- Thorax
- Publisher
- BMJ
- Published
- 2016-08-24
- Volume
- 71
- Issue
- 12
- Pages
- 1119-1129
- ISSN
- 0040-6376 (ISSN-L)
- 1468-3296
- PMC
- PMC5136722
- OpenAlex
- W2513274510
- Cited by
- 339
- References
- 42
- Citations by year
- 2026: 16 · 2025: 28 · 2024: 33 · 2023: 44 · 2022: 32 · 2021: 32 · 2020: 64 · 2019: 47 · 2018: 27 · 2017: 11 · 2016: 4
- FWCI
- 23.89
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc
- Locations
- Thorax (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- Flight Attendant Medical Research Institute (130033, 113380, YCSA113380)
- University of Pittsburgh
- National Institutes of Health
- National Heart, Lung, and Blood Institute (R01HL098528)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Smoking Behavior and Cessation
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Chronic Obstructive Pulmonary Disease (COPD) Research
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Inhalation and Respiratory Drug Delivery
- Keywords
- electronic cigarettes, nicotine, chronic nicotine exposure, chronic obstructive pulmonary disease, airway epithelial cells, bronchial epithelium, ciliary beat frequency, airway surface liquid, CFTR, Foxj1, KCNMA1, mucin production, cytokines, e-cigarette aerosol, lung disease
- MeSH terms
- Administration, Inhalation, Adult, Animals, Bronchi, Cells, Cultured, Cilia, Epithelial Cells, Female, Humans, Lung, Male, Mice, Inbred A, Middle Aged, Mucins, Nicotine, Peptide Hydrolases, Tobacco Use Disorder, Cytokines, Methacholine Chloride, Apoptosis, Pulmonary Disease, Chronic Obstructive, Electronic Nicotine Delivery Systems
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