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Cited by 175Muscle Physiology and DisordersChronic Obstructive Pulmonary Disease (COPD) ResearchNutrition and Health in Aging

Cytokine Profile in Quadriceps Muscles of Patients with Severe COPD

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Barreiro, E, Schols, A M W J, Polkey, M I, Galdiz, J B, Gosker, H R, Swallow, E B, Coronell, C, Gea, J +1 more·2007·Thorax
DOI 10.1136/thx.2007.078030
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Abstract

BACKGROUND: Systemic proinflammatory cytokines and oxidative stress have been described in association with peripheral muscle wasting and weakness of patients with severe chronic obstructive pulmonary disease (COPD), but their expression in skeletal muscle is unknown. The objectives of the present study were to determine muscle protein levels of selected cytokines in patients with COPD and to study their relationships with protein carbonylation as a marker of oxidative stress, quadriceps function and exercise capacity. METHODS: We conducted a cross sectional study in which 36 cytokines were detected using a human antibody array in quadriceps specimens obtained from 19 patients with severe COPD and seven healthy controls. Subsequently, selected cytokines (tumour necrosis factor (TNF)alpha, TNFalpha receptors I and II, interleukin (IL) 6, interferon gamma, transforming growth factor (TGF) beta and vascular endothelial growth factor (VEGF)), as well as protein carbonylation (oxidative stress index) were determined using an enzyme linked immunosorbent assay (ELISA) in all muscles. RESULTS: Compared with controls, the vastus lateralis of patients with COPD showed significantly lower protein ELISA levels of TNFalpha, which positively correlated with their quadriceps function, TNFalpha receptor II and VEGF. Protein ELISA levels of IL6, interferon gamma and TGFbeta did not differ between patients and controls. Quadriceps protein carbonylation was greater in patients and inversely correlated with quadriceps strength among them. CONCLUSIONS: These findings do not support the presence of a proinflammatory environment within the quadriceps muscles of clinically and weight stable patients with severe COPD, despite evidence for increased oxidative stress and the presence of muscle weakness.

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  • In muscle from COPD patients, IL-6 has been reported as unchanged at the transcriptional level at both stable and exacerbation states compared to healthy controls.

    “In muscle from COPD patients, IL-6 has been reported as unchanged at the transcriptional level (at both stable and exacerbation states) compared to that of healthy controls [27,28].”

    From Main Pathogenic Mechanisms and Recent Advances in COPD Peripheral Skeletal Muscle Wasting (2023) · cites this paper as [27]

    The 'Expression of IL6, Interferon c and TGFb' section reports no difference in quadriceps IL-6 between COPD patients and controls, but it measured protein by ELISA, not transcription. 'METHODS Subjects' identifies the patients as having stable COPD; this paper does not report an exacerbation-state comparison. Page numbers were not available in our copy. (LLM, conf=0.90)

    Experimental — accuracy not yet measured

  • IFN-γ has been reported as unchanged at the protein level in COPD quadriceps compared to controls, while a recent transcriptional analysis shows a globally downregulated interferon response in COPD quadriceps.

    “IFN-γ has been reported as unchanged at the protein level in COPD quadriceps compared to controls [27]. However, a recent transcriptional analysis shows a globally downregulated interferon response in COPD quadriceps [56].”

    From Main Pathogenic Mechanisms and Recent Advances in COPD Peripheral Skeletal Muscle Wasting (2023) · cites this paper as [27]

  • In the quadriceps of COPD patients, muscular TNF-α levels have been reported as significantly elevated, decreased, and unaltered, or even undetected at the protein level.

    “with regards to muscular TNF-α, significantly elevated, decreased, and unaltered levels have been reported in the quadriceps of COPD patients [27,28,29], or even undetected at the protein level [30,31]”

    From Main Pathogenic Mechanisms and Recent Advances in COPD Peripheral Skeletal Muscle Wasting (2023) · cites this paper as [27]

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Authors
Barreiro, E., Schols, A. M. W. J., Polkey, M. I., Galdiz, J. B., Gosker, H. R., Swallow, E. B., Coronell, C., Gea, J., & on behalf of the ENIGMA in COPD project
Affiliations
  • Universitat Pompeu Fabra (ES)
  • Maastricht University Medical Centre (NL)
  • Royal Brompton Hospital (GB)
Corresponding author
Barreiro, E
ORCID
Venue
Thorax
Publisher
BMJ
Published
2007-09-17
Volume
63
Issue
2
Pages
100-107
ISSN
0040-6376 (ISSN-L)
DOI
10.1136/thx.2007.078030
OpenAlex
W2163559718
Cited by
175
References
2
Citations by year
2026: 2 · 2025: 3 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 3 · 2019: 5 · 2018: 7 · 2017: 8 · 2016: 17 · 2015: 11 · 2014: 18 · 2013: 16 · 2012: 12
FWCI
5.79
Type
journal article
Language
en
Open access
Locations
  • Thorax (publishedVersion)
  • PubMed (publishedVersion)
  • Research Publications (Maastricht University) (publishedVersion, other-oa)
  • HighWire Press Open Archive (submittedVersion)
  • Research Publications (Maastricht University) (submittedVersion, other-oa)
Funders
European Commission
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Chronic Obstructive Pulmonary Disease (COPD) Research
  • Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
Keywords
chronic obstructive pulmonary disease, quadriceps, skeletal muscle inflammation, cytokines, TNF-α, TNF receptor 2, vascular endothelial growth factor, interleukin-6, interferon-gamma, TGF-beta, protein carbonylation, oxidative stress, muscle weakness, muscle atrophy, quadriceps strength, exercise capacity
MeSH terms
Aged, Biopsy, Needle, Cross-Sectional Studies, Enzyme-Linked Immunosorbent Assay, Humans, Immunohistochemistry, Male, Middle Aged, Biomarkers, Cytokines, Oxidative Stress, Muscle Weakness, Pulmonary Disease, Chronic Obstructive, Microarray Analysis, Quadriceps Muscle

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