Using transcriptomics to examine the physiological status of wild-caught walleye (Sander vitreus)
Open accessAbstract
Lake Winnipeg, Manitoba, is Canada’s second largest commercial inland freshwater fishery, and concern over collapse of the walleye fishery is growing. Molecular techniques have been increasingly used to study responses of wild organisms to environmental and anthropogenic stressors. The present study used transcriptomics to examine the physiology of wild-caught walleye ( Sander vitreus (Mitchell, 1818)) across Lake Winnipeg using non-lethal techniques. Gill transcriptomes of walleye sampled from the north and south basins of Lake Winnipeg, and the channel connecting them, exhibited distinct profiles implicating regionally specific biological responses. North basin walleye exhibited transcriptomic responses indicative of exposure to environmental stressors. Transcriptomic patterns suggested a shift to increased reliance on anaerobic metabolism and up-regulation of hypoxia-sensitive genes in north basin fish, possibly representing exposure to low-oxygen conditions. Exposure to environmental stressors may also have driven increases in gene transcripts associated with proteasomal catabolism, DNA repair, molecular chaperones, and ion regulation. North basin fish also exhibited transcriptomic patterns indicative of gill remodeling via regulation of the mTOR pathway, cytoskeleton reorganization, and fatty acid synthesis. Our results highlight the complexity of examining wild fish across environmental gradients but also the potential use of molecular techniques in elucidating organismal sensitivity to local environmental factors.
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- Authors
- Jeffrey, J. D., Thorstensen, M. J., Enders, E. C., Treberg, J. R., & Jeffries, K. M.
- Affiliations
- University of Manitoba (CA)
- Institut National de la Recherche Scientifique (CA)
- Corresponding author
- Jeffrey, Jennifer D.
- Venue
- FACETS
- Publisher
- Canadian Science Publishing
- Published
- 2023-01-01
- Volume
- 8
- Pages
- 1-15
- ISSN
- 2371-1671 (ISSN-L)
- OpenAlex
- W4386037693
- Cited by
- 22
- References
- 50
- Citations by year
- 2026: 6 · 2025: 8 · 2024: 7 · 2023: 1
- FWCI
- 3.56
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- FACETS (publishedVersion, cc-by)
- EspaceINRS (National Institute for Scientific Research (Canada)) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Genome Canada
- Canada Research Chairs (#223744)
- Natural Sciences and Engineering Research Council of Canada (05479, 06052)
- Fisheries and Oceans Canada
- Topics
- Physical Sciences › Environmental Science › Ecology › Physiological and biochemical adaptations
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Reproductive biology and impacts on aquatic species
- Keywords
- walleye, Lake Winnipeg, gill transcriptome, hypoxia, environmental stressors, anaerobic metabolism, DNA repair, ion regulation, mTOR signaling, fatty acid synthesis, cytoskeleton, fish physiology
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