Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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MIYAJIMA, T., Tsujino, T., Saito, K., & Yokoyama, M. (2001) · Hypertension Research · doi:10.1291/hypres.24.537 · Cited by 45
Effect of supplementing different oils: Linseed, sunflower and soybean, on animal performance, carcass characteristics, meat quality and fatty acid profile of veal from “Rubia Gallega” calves
González, L. M., Moreno, T., Bispo, E., Dugan, M. E. R., & Franco, D. (2013) · Meat Science · doi:10.1016/j.meatsci.2013.09.027 · Cited by 46
Solanum nigrum L. Seeds as an Alternative Source of Edible Lipids and Nutriment in Congo Brazzaville
Nzikou, J. M., Mvoula-Tsi, M., Matos, L., et al. (2007) · Journal of Applied Sciences · doi:10.3923/jas.2007.1107.1115 · Cited by 46
The glutathione-dependent system of antioxidant defense is not modulated by temperature acclimation in muscle tissues from striped bass, Morone saxatilis
Grim, J. M., Simonik, E. A., Semones, M. C., Kuhn, D. E., & Crockett, E. L. (2012) · Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology · doi:10.1016/j.cbpa.2012.11.018 · Cited by 46
Apoptotic Effect of Sphingosine 1-Phosphate and Increased Sphingosine 1-Phosphate Hydrolysis on Mesangial Cells Cultured at Low Cell Density
Gennero, I., Fauvel, J., Niéto, M., et al. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m108933200 · Cited by 46
Composição centesimal e valor protéico de levedura residual da fermentação etanólica e de seus derivados
Yamada, E. A., Alvim, I. D., Santucci, M. C. C., et al. (2003) · Revista de Nutrição · doi:10.1590/s1415-52732003000400006 · Cited by 46
Abnormal fatty alcohol metabolism in cultured keratinocytes from patients with Sjögren-Larsson syndrome
Rizzo, W. B., Craft, D. A., Somer, T., et al. (2007) · Journal of Lipid Research · doi:10.1194/jlr.m700469-jlr200 · Cited by 46
Abundance, distribution and δ 13 C analysis of microbial phospholipid-derived fatty acids in a black shale weathering profile
Petsch, S. T., Edwards, K. J., & Eglinton, T. I. (2003) · Organic Geochemistry · doi:10.1016/s0146-6380(03)00040-8 · Cited by 46
Effects of Hydrostatic Pressure and Temperature on Growth and Lipid Composition of the Inner Membrane of BarotolerantPseudomonassp. BT1 Isolated from the Deep-sea
KANEKO, H., Takami, H., Inoue, A., et al. (2000) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.64.72 · Cited by 46
Integrated approach to sediment pollution: A case study in the Gulf of Trieste
Cibic, T., Acquavita, A., Aleffi, F., et al. (2008) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2008.05.009 · Cited by 46
A revised biosynthetic pathway for phosphatidylinositol in Mycobacteria
Morii, H., Ogawa, M., Fukuda, K., Taniguchi, H., & Koga, Y. (2010) · The Journal of Biochemistry · doi:10.1093/jb/mvq093 · Cited by 46
Correlation of long-range membrane order with temperature-dependent growth characteristics of parent and a cold-sensitive, branched-chain-fatty-acid-deficient mutant of Listeria monocytogenes
Jones, S., Drouin, P., Wilkinson, B. J., et al. (2002) · Archives of Microbiology · doi:10.1007/s00203-001-0380-4 · Cited by 46
Identification of hepatic peroxisomal phospholipase A2and characterization of arachidonic acid‐containing choline glycerophospholipids in hepatic peroxisomes
Yang, J., Han, X., & Gross, R. W. (2003) · FEBS Letters · doi:10.1016/s0014-5793(03)00581-7 · Cited by 45
A possible role of ProP, ProU and CaiT in osmoprotection of Escherichia coli by carnitine
Verheul, A., Wouters, J. A., ROMBOUTS, F. M., et al. (1998) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.1998.tb05269.x · Cited by 46
Different oxidized phospholipid molecules unequally affect bilayer packing
Megli, F. M., & Russo, L. (2007) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2007.09.014 · Cited by 47
Seasonal study of the lipid composition in different tissues of the common octopus ( Octopus vulgaris )
Sieiro, M. P., Aubourg, S. P., & Rocha, F. (2006) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.200500322 · Cited by 46
Alterations to surfactant precede physiological deterioration during high tidal volume ventilation
Maruscak, A. A., Vockeroth, D., Girardi, B. L., et al. (2008) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00528.2007 · Cited by 46
Association of MYF5 and MYOD1 Gene Polymorphisms and Meat Quality Traits in Large White × Meishan F2 Pig Populations
Liu, M., Peng, J., Xu, D., et al. (2008) · Biochemical Genetics · doi:10.1007/s10528-008-9187-1 · Cited by 46
Cis-9,trans-11-conjugated linoleic acid but not its precursortrans-vaccenic acid attenuate inflammatory markers in the human colonic epithelial cell line Caco-2
Reynolds, C. M., Loscher, C. E., MOLONEY, A. P., & Roche, H. M. (2008) · British Journal Of Nutrition · doi:10.1017/s0007114508894329 · Cited by 46
Influence of heating time and oxygen availability on lipid oxidation in meat emulsions
Andreo, A. I., Doval, M. M., Romero, A., et al. (2003) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.200390042 · Cited by 46
Analysis of the role of the PAC1 receptor in neutrophil recruitment, acute-phase response, and nitric oxide production in septic shock
Martínez, C., Juarranz, Y., Abad, C., et al. (2005) · Journal of Leukocyte Biology · doi:10.1189/jlb.0704432 · Cited by 46
Deep Subsurface Microbial Biomass and Community Structure in Witwatersrand Basin Mines
Pfiffner, S. M., Cantu, J., Smithgall, A., Peacock, A. D., White, D. C., Moser, D. P., Onstott, T. C., & van Heerden, E. (2006) · Geomicrobiology Journal · doi:10.1080/01490450600875712 · Cited by 46
Ethanol promotes neuronal apoptosis by inhibiting phosphatidylserine accumulation
Akbar, M. D., Baick, J., Calderón, F. H., Wen, Z., & Kim, H. (2006) · Journal of Neuroscience Research · doi:10.1002/jnr.20744 · Cited by 47
Effect of Mediterranean and Occidental Diets, and Red Wine, on Plasma Fatty Acids in Humans: An Intervention Study
Urquiaga, I., Guasch, V., Marshall, G., Martín, A. S., Castillo, O., Rozowski, J., & Leighton, F. (2004) · Biological Research · doi:10.4067/s0716-97602004000200012 · Cited by 46
Synthesis of lipids in the fat body of Gryllus bimaculatus: Age‐dependency and regulation by adipokinetic hormone
Lorenz, M. W. (2001) · Archives of Insect Biochemistry and Physiology · doi:10.1002/arch.1052 · Cited by 46
Building a multipurpose insertional mutant library for forward and reverse genetics in Chlamydomonas
Cheng, X., Gai, L., Ke, W., Zhao, L., Lv, B., Ma, X., Xu, N., Xia, X., Deng, X., Zheng, C., et al. (2017) · Plant Methods · doi:10.1186/s13007-017-0183-5 · Cited by 48
[49] Modification of bacterial lipoproteins
Sankaran, K., Gupta, S. D., & Wu, H. C. (1995) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(95)50105-3 · Cited by 46
Transition of Nitro Musks and Polycyclic Musks Into Human Milk
Liebl, B., Mayer, R. L., Ommer, S., et al. (2005) · Kluwer Academic Publishers eBooks · doi:10.1007/0-306-46830-1_26 · Cited by 46

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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