Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 14,301–14,400 of 48,846

Exogenous Polyunsaturated Fatty Acids Impact Membrane Remodeling and Affect Virulence Phenotypes among Pathogenic Vibrio Species
Moravec, A., Siv, A. W., Hobby, C. R., Lindsay, E. N., Norbash, L. V., Shults, D. J., Symes, S. J. K., & Giles, D. K. (2017) · Applied and Environmental Microbiology · doi:10.1128/aem.01415-17 · Cited by 45
The effects of slaughter age on carcass and meat quality of Fabrianese lambs
Polidori, P., Pucciarelli, S., Cammertoni, N., et al. (2017) · Small Ruminant Research · doi:10.1016/j.smallrumres.2017.08.012 · Cited by 45
Group III phospholipase A2 promotes colitis and colorectal cancer
Murase, R., Taketomi, Y., Miki, Y., Nishito, Y., Saito, M., Fukami, K., Yamamoto, K., & Murakami, M. (2017) · Scientific Reports · doi:10.1038/s41598-017-12434-z · Cited by 44
Improvement in modular scalability of polymeric thin-film photobioreactor for autotrophic culturing of Haematococcus pluvialis using industrial flue gas
Choi, Y. Y., Hong, M. E., Jin, E., Woo, H. M., & Sim, S. J. (2017) · Bioresource Technology · doi:10.1016/j.biortech.2017.10.060 · Cited by 45
Genome-scale modeling and transcriptome analysis of Leuconostoc mesenteroides unravel the redox governed metabolic states in obligate heterofermentative lactic acid bacteria
Koduru, L., Kim, Y., Bang, J., Lakshmanan, M., Han, N. S., & Lee, D. (2017) · Scientific Reports · doi:10.1038/s41598-017-16026-9 · Cited by 48
Shared and distinct lipid-lipid interactions in plasma and affected tissues in a diabetic mouse model
Sas, K. M., Lin, J., Rajendiran, T. M., Soni, T., Nair, V., Hinder, L. M., Jagadish, H. V., Gardner, T. W., Abcouwer, S. F., Brosius, F. C., et al. (2017) · Journal of Lipid Research · doi:10.1194/jlr.m077222 · Cited by 45
The effects of abscisic acid, salicylic acid and jasmonic acid on lipid accumulation in two freshwater Chlorella strains
Wu, G., Gao, Z., Du, H., Lin, B., Yan, Y., Li, G., Guo, Y., Fu, S., Wei, G., Wang, M., et al. (2017) · The Journal of General and Applied Microbiology · doi:10.2323/jgam.2017.06.001 · Cited by 47
ChREBP-Knockout Mice Show Sucrose Intolerance and Fructose Malabsorption
Kato, T., Iizuka, K., Takao, K., Horikawa, Y., Kitamura, T., & Takeda, J. (2018) · Nutrients · doi:10.3390/nu10030340 · Cited by 46
Stereoselective bioaccumulation of chiral PCB 91 in earthworm and its metabolomic and lipidomic responses
He, Z., Wang, Y., Zhang, Y., et al. (2018) · Environmental Pollution · doi:10.1016/j.envpol.2018.03.060 · Cited by 45
The effect of replacing egg yolk with sesame–peanut defatted meal milk on the physicochemical, colorimetry, and rheological properties of low‐cholesterol mayonnaise
Karshenas, M., Goli, M., & Zamindar, N. (2018) · Food Science & Nutrition · doi:10.1002/fsn3.616 · Cited by 45
Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains
Wong, E. H. J., Ng, C. G., Goh, K., Vadivelu, J., Ho, B., & Loke, M. F. (2018) · Scientific Reports · doi:10.1038/s41598-018-19697-0 · Cited by 44
Nutritional value of the Tunisian mussel Mytilus galloprovincialis with a special emphasis on lipid quality
Cherifi, H., Ajjabi, L. C., & Sadok, S. (2018) · Food Chemistry · doi:10.1016/j.foodchem.2018.06.075 · Cited by 45
Contemporary lipidomic analytics: opportunities and pitfalls
Giles, C., Takechi, R., Lam, V., Dhaliwal, S. S., & Mamo, J. C. L. (2018) · Progress in Lipid Research · doi:10.1016/j.plipres.2018.06.003 · Cited by 45
Establishment of stable microalgal-bacterial consortium in liquid digestate for nutrient removal and biomass accumulation
Jiang, Y., Wang, H., Zhao, C., Huang, F., Deng, L., & Wang, W. (2018) · Bioresource Technology · doi:10.1016/j.biortech.2018.07.142 · Cited by 44
A systematic comparison of 25 Tunisian plant species based on oil and phenolic contents, fatty acid composition and antioxidant activity
Ksouda, G., Hajji, M., Sellimi, S., Merlier, F., Falcimaigne-Cordin, A., Nasri, M., & Thomasset, B. (2018) · Industrial Crops and Products · doi:10.1016/j.indcrop.2018.07.008 · Cited by 47
Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin
Nitsche, J., Josts, I., Heidemann, J., et al. (2018) · Communications Biology · doi:10.1038/s42003-018-0203-7 · Cited by 45
Analytical Considerations of Stable Isotope Labelling in Lipidomics
Triebl, A., & Wenk, M. R. (2018) · Biomolecules · doi:10.3390/biom8040151 · Cited by 47
Fatty acid, physicochemical composition and sensory attributes of meat from lambs fed diets containing licuri cake
Costa, J. B., Oliveira, R. L., Silva, T. M., Barbosa, A. M., Borja, M. S., de Pellegrini, C. B., Oliveira, V. D. S., Ribeiro, R. D. X., & Bezerra, L. R. (2018) · PLoS ONE · doi:10.1371/journal.pone.0206863 · Cited by 47
Development of high biomass and lipid yielding medium for newly isolated Rhodotorula mucilaginosa
Prabhu, A. A., Gedela, R., Bharali, B., Deshavath, N. N., & Dasu, V. V. (2018) · Fuel · doi:10.1016/j.fuel.2018.11.088 · Cited by 45
Non-conventional Tropical Fruits: Characterization, Antioxidant Potential and Carotenoid Bioaccessibility
Berni, P., Campoli, S. S., Negri, T. C., et al. (2019) · Plant Foods for Human Nutrition · doi:10.1007/s11130-018-0710-1 · Cited by 45
Invited review: Authentication of milk by direct and indirect analysis of triacylglycerol molecular species
Cossignani, L., Pollini, L., & Blasi, F. (2019) · Journal of Dairy Science · doi:10.3168/jds.2019-16318 · Cited by 46
A Comprehensive Functional Characterization of Escherichia coli Lipid Genes
Jeucken, A., Molenaar, M. R., van de Lest, C. H. A., Jansen, J. W. A., Helms, J. B., & Brouwers, J. F. H. M. (2019) · Cell Reports · doi:10.1016/j.celrep.2019.04.018 · Cited by 47
Dietary Profiles, Nutritional Biochemistry Status, and Attention-Deficit/Hyperactivity Disorder: Path Analysis for a Case-Control Study
Wang, L., Yu, Y., Fu, M.-L., Yeh, W., Hsu, J.-L., Yang, Y., Yang, H., Huang, S., Wei, I.-L., Chen, W. J., et al. (2019) · Journal of Clinical Medicine · doi:10.3390/jcm8050709 · Cited by 43
Sphingomyelin Biosynthesis Is Essential for Phagocytic Signaling during Mycobacterium tuberculosis Host Cell Entry
Niekamp, P., Guzman, G., Leier, H. C., Rashidfarrokhi, A., Richina, V., Pott, F., Barisch, C., Holthuis, J. C. M., & Tafesse, F. (2021) · mBio · doi:10.1128/mbio.03141-20 · Cited by 45
“Cells were then washed two times with PBS and lipid extraction was done following the Bligh and Dyer method ( 40 ).”
Method
Alleviation of cadmium-induced genotoxicity and cytotoxicity by calcium chloride in faba bean (Vicia faba L. var. minor) roots
Nouairi, I., Jalali, K., Essid, S., et al. (2019) · Physiology and Molecular Biology of Plants · doi:10.1007/s12298-019-00681-5 · Cited by 45
Does lipid-correction introduce biases into isotopic mixing models? Implications for diet reconstruction studies
Arostegui, M. C., Schindler, D. E., & Holtgrieve, G. W. (2019) · Oecologia · doi:10.1007/s00442-019-04525-7 · Cited by 45
Effect of temperature on growth and fatty acids profile of the biodiesel producing microalga Scenedesmus acutus
El-Sheekh, M. M., Abomohra, A., El-Azim, M. A., & Abou-Shanab, R. A. I. (2017) · BASE · doi:10.25518/1780-4507.15291 · Cited by 44
Biocomponent-based microalgal transformations into biofuels during the pretreatment and fermentation process
Ha, G.-S., El-Dalatony, M. M., Kim, D.-H., Salama, E., Kurade, M. B., Roh, H., Abomohra, A., & Jeon, B. (2020) · Bioresource Technology · doi:10.1016/j.biortech.2020.122809 · Cited by 44
Effect of seasonal variations on fatty acid composition and nutritional profiles of siluriformes fish species from the amazon basin
de Souza, A. F. L., Petenuci, M. E., Camparim, R., Visentainer, J. V., & da Silva, A. J. I. (2020) · Food Research International · doi:10.1016/j.foodres.2020.109051 · Cited by 54
Domesticated Populations of Codium tomentosum Display Lipid Extracts with Lower Seasonal Shifts than Conspecifics from the Wild—Relevance for Biotechnological Applications of this Green Seaweed
Rey, F., Cartaxana, P., Melo, T., Calado, R., Pereira, R., Abreu, H., Domingues, P. M., Cruz, S., & Domingues, M. R. (2020) · Marine Drugs · doi:10.3390/md18040188 · Cited by 44
“The Bligh and Dyer method [59] was modified to extract total lipids from C.”
Method
CRY1‐CBS binding regulates circadian clock function and metabolism
Cal‐Kayitmazbatir, S., Kulkoyluoglu‐Cotul, E., Growe, J., Selby, C. P., Rhoades, S. D., Malik, D. M., Öner, H., Asimgil, H., Francey, L. J., Sancar, A., et al. (2020) · FEBS Journal · doi:10.1111/febs.15360 · Cited by 45
Development of Fish Oil-Loaded Microcapsules Containing Whey Protein Hydrolysate as Film-Forming Material for Fortification of Low-Fat Mayonnaise
Rahmani-Manglano, N. E., González-Sánchez, I., García‐Moreno, P. J., Espejo-Carpio, F. J., Jacobsen, C., & Guadix, E. M. (2020) · Foods · doi:10.3390/foods9050545 · Cited by 45
“Peroxide Value (PV) Fish oil was extracted from the microcapsules, as described by Bligh and Dyer [21] using a reduced amount of chloroform/methanol (1:1, w/w) solvent.”
Method
Light Intensity Improves Growth, Lipid Productivity, and Fatty Acid Profile of Chlorococcum oleofaciens (Chlorophyceae) for Biodiesel Production
Rayati, M., Islami, H. R., & Mehrgan, M. S. (2020) · BioEnergy Research · doi:10.1007/s12155-020-10144-5 · Cited by 45
Physicochemical properties of pumpkin ( Cucurbita pepo L.) seed kernel flour and its utilization in beef meatballs as a fat replacer and functional ingredient
Öztürk, T., & Turhan, S. (2020) · Journal of Food Processing and Preservation · doi:10.1111/jfpp.14695 · Cited by 45
Bioconversion of durian (Durio zibethinus Murr.) peel hydrolysate into biodiesel by newly isolated oleaginous yeast Rhodotorula mucilaginosa KKUSY14
Siwina, S., & Leesing, R. (2020) · Renewable Energy · doi:10.1016/j.renene.2020.08.138 · Cited by 45
Analysis of serum lysophosphatidylethanolamine levels in patients with non-alcoholic fatty liver disease by liquid chromatography-tandem mass spectrometry
Yamamoto, Y., Sakurai, T., Chen, Z., Furukawa, T., Gowda, S. G. B., Wu, Y., Nouso, K., Fujii, Y., Yoshikawa, Y., Chiba, H., et al. (2020) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-020-02996-9 · Cited by 45
“Lipid extraction was performed according to the Bligh and Dyer method with minor modifications [23].”
Method
Insulin receptor deficiency reduces lipid synthesis and reproductive function in the insect Rhodnius prolixus
Silva-Oliveira, G., Paula, I. F. D., Medina, J. M., et al. (2020) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2020.158851 · Cited by 45
Cultivation of green microalgae by recovering aqueous nutrients in hydrothermal carbonization process water of biomass wastes
Tarhan, S. Z., Koçer, A. T., Özçimen, D. B., & Gökalp, İ. (2020) · Journal of Water Process Engineering · doi:10.1016/j.jwpe.2020.101783 · Cited by 44
Dry-aged beef manufactured in Japan: Microbiota identification and their effects on product characteristics
Mikami, N., Toyotome, T., Yamashiro, Y., et al. (2020) · Food Research International · doi:10.1016/j.foodres.2020.110020 · Cited by 45
Structure of dual BON-domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation
Bryant, J. A., Morris, F. C., Knowles, T. J., Maderbocus, R., Heinz, E., Boelter, G., Alodaini, D., Colyer, A., Wotherspoon, P. J., Staunton, K. A., et al. (2020) · eLife · doi:10.7554/elife.62614 · Cited by 46
Phospholipid translocation captured in a bifunctional membrane protein MprF
Song, D., Jiao, H., & Liu, Z. (2021) · Nature Communications · doi:10.1038/s41467-021-23248-z · Cited by 45
Bioethanol Production from Green Alga Chlorococcum minutum through Reduced Graphene Oxide-Supported Platinum-Ruthenium (Pt-Ru/RGO) Nanoparticles
Varaprasad, D., Raghavendra, P., Sudha, N. R., Sarma, L. S., Parveen, S., Panchangam, S. C., Chandra, M. S., & Chandrasekhar, T. (2021) · BioEnergy Research · doi:10.1007/s12155-021-10282-4 · Cited by 45
CD1a selectively captures endogenous cellular lipids that broadly block T cell response
Cotton, R. N., Wegrecki, M., Cheng, T., Chen, Y., Veerapen, N., Nours, J. L., Orgill, D. P., Pomahač, B., Talbot, S. G., Willis, R. A., et al. (2021) · The Journal of Experimental Medicine · doi:10.1084/jem.20202699 · Cited by 46
“For lipid elution, 20–200 μg of CD1 protein was extracted in 15-ml glass tubes using chloroform, methanol, and water (Bligh and Dyer, 1959).”
Method
Lipidomic and transcriptional analysis of the linoleoyl-omega-hydroxyceramide biosynthetic pathway in human psoriatic lesions
Tyrrell, V. J., Ali, F., Boeglin, W. E., Andrews, R., Burston, J. J., Birchall, J. C., Ingram, J. R., Murphy, R. C., Piguet, V., BRASH, A. R., et al. (2021) · Journal of Lipid Research · doi:10.1016/j.jlr.2021.100094 · Cited by 44
Interrogating in vivo T-cell metabolism in mice using stable isotope labeling metabolomics and rapid cell sorting
Sheldon, R. D., Ma, E. H., DeCamp, L. M., Williams, K. S., & Jones, R. G. (2021) · Nature Protocols · doi:10.1038/s41596-021-00586-2 · Cited by 42
The tertiary structure of the human Xkr8–Basigin complex that scrambles phospholipids at plasma membranes
Sakuragi, T., Kanai, R., Tsutsumi, A., Narita, H., Onishi, E., Nishino, K., Miyazaki, T., Baba, T., Kosako, H., Nakagawa, A., et al. (2021) · Nature Structural & Molecular Biology · doi:10.1038/s41594-021-00665-8 · Cited by 46
Gut microbiota modulation by jabuticaba peel and its effect on glucose metabolism via inflammatory signaling
Filho, P. S. L., Baseggio, A. M., Vuolo, M. M., Reguengo, L. M., Biasoto, A. C. T., Corrêa, L. C., Bogusz, S., Cagnon, V. H. A., Cazarin, C. B. B., & Maróstica, M. R. (2022) · Current Research in Food Science · doi:10.1016/j.crfs.2022.02.001 · Cited by 47
“…(constant drying at 100 ◦C), total protein (Kjeldahl 6.25 conversion factor), ashes (muffle incineration at 550 ◦C) (AOAC, 1995), total lipids (Bligh and Dyer, 1959), and carbohydrate was calculated by the following formula (%carbohydrate = 100 - %humidity - %total protein - %ash - %total lipids).”
MethodInfluential
Biomass production and phycoremediation of microalgae cultivated in polluted river water
Ummalyma, S. B., & Singh, A. (2022) · Bioresource Technology · doi:10.1016/j.biortech.2022.126948 · Cited by 46
A recipe for a good clinical pulmonary surfactant
Pérez‐Gil, J. (2022) · Biomedical Journal · doi:10.1016/j.bj.2022.03.001 · Cited by 45
“Those extractive procedures with solvents such as chloroform/methanol mixtures are typically used by biochemists to obtain the lipid components of biological samples [40], and it was therefore assumed that the lipid moiety of surfactant was the main responsible for their surface tension reduction capabilities.”
Method
Lowering n-6/n-3 Ratio as an Important Dietary Intervention to Prevent LPS-Inducible Dyslipidemia and Hepatic Abnormalities in ob/ob Mice
Park, S., Lee, J., Lee, J., Lee, J. K., Byun, J., Kim, I., & Ha, J. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23126384 · Cited by 45
“Lipids were extracted from the liver and EAT as previously described by Bligh and Dyer [80], with slight modifications.”
Method
Unanswered issues on decarbonizing the aviation industry through the development of sustainable aviation fuel from microalgae
Rony, Z. I., Mofijur, M., Hasan, M. M., Ahmed, S. F., AlMomani, F. A., Rasul, M. G., Jahirul, M. I., Show, P. L., Kalam, M. A., & Mahlia, T. M. I. (2022) · Fuel · doi:10.1016/j.fuel.2022.126553 · Cited by 46
Development of a high-throughput in vitro intestinal lipolysis model for rapid screening of lipid-based drug delivery systems
Mosgaard, M. D., Sassene, P. J., Mu, H., Rades, T., & Müllertz, A. (2015) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2015.06.028 · Cited by 45
Cortisol affects metabolic and ionoregulatory responses to a different extent depending on feeding ration in common carp, Cyprinus carpio
Liew, H. J., Fazio, A., Faggio, C., et al. (2015) · Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology · doi:10.1016/j.cbpa.2015.07.011 · Cited by 45
NaxD is a deacetylase required for lipid A modification and F rancisella pathogenesis
Llewellyn, A. C., Zhao, J., Song, F., et al. (2012) · Molecular Microbiology · doi:10.1111/mmi.12004 · Cited by 45
Respiratory syncytial virus infection of human mononuclear phagocytes stimulates synthesis of platelet-activating factor.
Villani, A., Cirino, N. M., Baldi, E., KESTER, M. A., McFadden, E. R., & Panuska, J. R. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)67619-2 · Cited by 45
Glycerol reorientation during the conversion of phosphatidylglycerol to bis(monoacylglycerol)phosphate in macrophage-like RAW 264.7 cells.
Thornburg, T., Miller, C., Thuren, T. Y., King, L. M., & Waite, M. B. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)89576-3 · Cited by 46
Appearance of monoglyceride and triglyceride in the cell envelope of Escherichia coli mutants defective in diglyceride kinase.
Rotering, H., & Raetz, C. R. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)82028-6 · Cited by 45
Signaling events mediating activation of brain ethanolamine plasmalogen hydrolysis by ceramide
Latorre, E., Collado, M. P., Fernández, I., Aragonés, M. D., & Catalán, R. E. (2002) · European Journal of Biochemistry · doi:10.1046/j.1432-1033.2003.03356.x · Cited by 45
Fatty acid as markers to demonstrating trophic relationships among diatoms, rotifers and green-lipped mussels
Shin, P. K. S., Yip, K. M., Xu, W., Wong, W. H., & Cheung, S. G. (2008) · Journal of Experimental Marine Biology and Ecology · doi:10.1016/j.jembe.2008.01.002 · Cited by 42
Differential effect of surfactant and its saturated phosphatidylcholines on human blood macrophages
Gille, C., Spring, B., Bernhard, W., Gebhard, C. E., Basile, D., Lauber, K., Poets, C. F., & Orlikowsky, T. W. (2006) · Journal of Lipid Research · doi:10.1194/jlr.m600451-jlr200 · Cited by 45
Effects of Hydrostatic Pressure on Growth and Luminescence of a Moderately-Piezophilic Luminous Bacteria Photobacterium phosphoreum ANT-2200
Martini, S., Ali, B. A., Garel, M., et al. (2013) · PLoS ONE · doi:10.1371/journal.pone.0066580 · Cited by 45
Effect of biological variables and capture period on the proximate composition and fatty acid composition of the dorsal muscle tissue of Hypophthalmus edentatus (Spix, 1829)
de Oliveira, E. R. N., Agostinho, Â. A., & Matsushita, M. (2003) · Brazilian Archives of Biology and Technology · doi:10.1590/s1516-89132003000100015 · Cited by 45
Insulin Activates Phospholipase C-γ1 via a PI-3 Kinase Dependent Mechanism in 3T3-L1 Adipocytes
Eichhorn, J., Kayali, A. G., Austin, D. A., & Webster, N. J. G. (2001) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2001.4616 · Cited by 45
Detection and cultivation of indigenous microorganisms in Mesozoic claystone core samples from the Opalinus Clay Formation (Mont Terri Rock Laboratory)
Mauclaire, L., McKenzie, J. A., Schwyn, B., & Bossart, P. (2006) · Physics and Chemistry of the Earth Parts A/B/C · doi:10.1016/j.pce.2005.12.010 · Cited by 45
Optimization of the Enzymatic Hydrolysis of Mussel Meat
da Silva, V. M., Park, K. J., & Hubinger, M. D. (2009) · Journal of Food Science · doi:10.1111/j.1750-3841.2009.01414.x · Cited by 45
Vitamin D3 and its metabolites in the tomato plant
Prema, T. P., & Raghuramulu, N. (1996) · Phytochemistry · doi:10.1016/0031-9422(95)00883-7 · Cited by 45
Diminished Lung Compliance and Elevated Surfactant Lipids and Proteins in Nutritionally Obese Young Rats
Inselman, L. S., Chander, A., & Spitzer, A. R. (2004) · Lung · doi:10.1007/s00408-003-1048-4 · Cited by 45
Extending the shelf-life and proximate composition stability of ready to eat foods in vacuum or modified atmosphere packaging
Murcia, M. A., Martínez‐Tomé, M., Nicolás, M. C., et al. (2003) · Food Microbiology · doi:10.1016/s0740-0020(03)00013-3 · Cited by 45
Determination of phenolic and other polar compounds in flaxseed oil using liquid chromatography coupled with time-of-flight mass spectrometry
Herchi, W., Sawalha, S., Arraéz-Román, D., et al. (2010) · Food Chemistry · doi:10.1016/j.foodchem.2010.10.070 · Cited by 45
Colloid centrifugation removes seminal plasma and cholesterol from boar spermatozoa
Kruse, R. L., Dutta, P. C., & Morrell, J. M. (2011) · Reproduction Fertility and Development · doi:10.1071/rd10260 · Cited by 45
Characteristic Oxidation Products of Choline Plasmalogens are Detectable in Cattle and Roe Deer Spermatozoa by MALDI‐TOF Mass Spectrometry
Fuchs, B., Müller, K., Göritz, F., et al. (2007) · Lipids · doi:10.1007/s11745-007-3108-7 · Cited by 45
Identification of AtPIS, a phosphatidylinositol synthase from Arabidopsis
Collin, S., Justin, A., Cantrel, C., et al. (1999) · European Journal of Biochemistry · doi:10.1046/j.1432-1327.1999.00378.x · Cited by 45
Lipid and protein structure analysis of frankfurters formulated with olive oil-in-water emulsion as animal fat replacer
Herrero, A. M., Carmona, P., Pintado, T., Jiménez-Colmenero, F., & Ruíz‐Capillas, C. (2012) · Food Chemistry · doi:10.1016/j.foodchem.2012.04.114 · Cited by 44
Characterisation of a traditional Finnish liver sausage and different types of Spanish liver pâtés: A comparative study
Estévez, M., Ventanas, J., Cava, R., et al. (2005) · Meat Science · doi:10.1016/j.meatsci.2005.05.018 · Cited by 45
Quantification of age pigments (lipofuscin)
Hammer, C., & Braum, E. (1988) · Comparative Biochemistry and Physiology Part B Comparative Biochemistry · doi:10.1016/0305-0491(88)90030-2 · Cited by 45
Evidence of a growth factor in some crustacean-based feed ingredients in diets for the giant tiger shrimp Penaeus monodon
Williams, K. C., Smith, D. M., Barclay, M. C., et al. (2005) · Aquaculture · doi:10.1016/j.aquaculture.2005.04.002 · Cited by 45
Chronic ANG II infusion increases plasma triglyceride level by stimulating hepatic triglyceride production in rats
Ran, J., Hirano, T., & Adachi, M. (2004) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00199.2004 · Cited by 45
Dopamine release in PC12 cells is mediated by Ca2+‐dependent production of ceramide via sphingomyelin pathway
Jeon, H. J., Lee, D. H., Kang, M. S., et al. (2005) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2005.03403.x · Cited by 45
Improved Extraction of Saturated Fatty Acids but not Omega‐3 Fatty Acids from Sheep Red Blood Cells Using a One‐Step Extraction Procedure
Clayton, E. H., Gulliver, C. E., Piltz, J. W., et al. (2012) · Lipids · doi:10.1007/s11745-012-3674-1 · Cited by 45
Distribution and dynamics of various forms of phosphorus in seawater: insights from field observations in the Pacific Ocean and a laboratory experiment
Suzumura, M., & Ingall, E. D. (2004) · Deep Sea Research Part I Oceanographic Research Papers · doi:10.1016/j.dsr.2004.05.001 · Cited by 45
The fatty acid composition of plasma cholesteryl esters and estimated desaturase activities in patients with nonalcoholic fatty liver disease and the effect of long-term ezetimibe therapy on these levels
Park, H., Hasegawa, G., Shima, T., Fukui, M., Nakamura, N., Yamaguchi, K., Mitsuyoshi, H., Minami, M., Yasui, K., Itoh, Y., et al. (2010) · Clinica Chimica Acta · doi:10.1016/j.cca.2010.07.012 · Cited by 46
DIFFERENCES IN LIPID PROFILE OF NEW ZEALAND MARINE SPECIES OVER FOUR SEASONS
McLean, C. H., & BULLING, K. R. (2005) · Journal of Food Lipids · doi:10.1111/j.1745-4522.2005.00026.x · Cited by 46
Damage detection in horse mackerel (Trachurus trachurus) during chilled storage
Aubourg, S. P. (2001) · Journal of the American Oil Chemists Society · doi:10.1007/s11746-001-0355-3 · Cited by 45
Changes in Oil Content of Transgenic Soybeans Expressing the Yeast SLC1 Gene
Rao, S. S., & Hildebrand, D. F. (2009) · Lipids · doi:10.1007/s11745-009-3337-z · Cited by 45
Protection against the photo‐induced inactivation of the photosystem II complex by abscisic acid
Saradhi, P. P., Suzuki, I., Katoh, A., et al. (2000) · Plant Cell & Environment · doi:10.1046/j.1365-3040.2000.00579.x · Cited by 45
The Yeast Phospholipid N-Methyltransferases Catalyzing the Synthesis of Phosphatidylcholine Preferentially Convert Di-C16:1 Substrates Both in Vivo and in Vitro
Boumann, H. A., Chin, P. T. K., Heck, A. J. R., et al. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m406517200 · Cited by 45
High pressure effects on the quality and preservation of cold-smoked dolphinfish (Coryphaena hippurus) fillets
Gómez‐Estaca, J., Gómez-Guillén, M. C., & Montero, P. (2006) · Food Chemistry · doi:10.1016/j.foodchem.2006.07.014 · Cited by 45
Ca2+-dependent mechanisms of cell injury in cultured cortical neurons
Castillo, M. R., & Babson, J. R. (1998) · Neuroscience · doi:10.1016/s0306-4522(98)00070-0 · Cited by 45
Orthogonal Activation of the Reengineered A3Adenosine Receptor (Neoceptor) Using Tailored Nucleoside Agonists
Gao, Z., Duong, H. T., Sonina, T., Kim, S., Rompaey, P. V., Calenbergh, S. V., Mamedova, L., Kim, H. O., Kim, M. J., Kim, A. Y., et al. (2006) · Journal of Medicinal Chemistry · doi:10.1021/jm050968b · Cited by 45
Activation of the Nuclear Receptor PXR Decreases Plasma LDL-Cholesterol Levels and Induces Hepatic Steatosis in LDL Receptor Knockout Mice
Hoekstra, M., Lammers, B., Out, R., Li, Z., Eck, M. V., & van Berkel, T. J. C. (2008) · Molecular Pharmaceutics · doi:10.1021/mp800131d · Cited by 45
Changes in phytochemical, antimicrobial and free radical scavenging activities of the Peruvian pepper tree (Schinus molle L.) as influenced by fruit maturation
Hosni, K., Jemli, M., Dziri, S., M’Rabet, Y., Ennigrou, A., Sghaier, A., Casabianca, H., Vulliet, E., Brahim, N. B., & Sebei, H. (2011) · Industrial Crops and Products · doi:10.1016/j.indcrop.2011.06.004 · Cited by 44
Evaluation of chitosan nanoparticles as a glazing material for cryogenically frozen shrimp
Solval, K. M., Rodezno, L. A. E., Moncada, M. L., et al. (2014) · LWT · doi:10.1016/j.lwt.2013.12.033 · Cited by 45
A method for radiolabeling Artemia with applications in studies of food intake, digestibility, protein and amino acid metabolism in larval fish
Morais, S. (2003) · Aquaculture · doi:10.1016/j.aquaculture.2003.09.005 · Cited by 45
Ryegrass straw component decomposition during mesophilic and thermophilic incubations
Horwáth, W. R., & Elliott, L. F. (1996) · Biology and Fertility of Soils · doi:10.1007/bf00334896 · Cited by 45
Diet compounds, glycemic index and obesity-related cardiac effects
Diniz, Y. S., Burneiko, R. M., Seiva, F. R. F., Aranha, F. Q., Galhardi, C. M., Filho, J. L. V. B. N., Mani, F., & Novelli, E. L. B. (2007) · International Journal of Cardiology · doi:10.1016/j.ijcard.2006.12.025 · Cited by 44
Nutritional Value of Seven Freshwater Fish Species From the Brazilian Pantanal
Filho, M. M. R., Ramos, M. I. L., Hiane, P. A., & de Souza, E. M. T. (2010) · Journal of the American Oil Chemists Society · doi:10.1007/s11746-010-1639-1 · Cited by 45
Omega-3 polyunsaturated fatty acids improve host response in chronicPseudomonas aeruginosalung infection in mice
Pierre, M., Husson, M.-O., Berre, R. L., et al. (2007) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00337.2006 · Cited by 45
Role of Squalene in the Organization of Monolayers Derived from Lipid Extracts of Halobacterium salinarum
Gilmore, S. F., Yao, A. I., Tietel, Z., et al. (2013) · Langmuir · doi:10.1021/la401412t · Cited by 45
Antiatherogenic properties of lipid fractions of raw and fried fish
Nomikos, T., Karantonis, H. C., SKARVELIS, C., et al. (2005) · Food Chemistry · doi:10.1016/j.foodchem.2005.01.060 · Cited by 45
Biochemical composition of crustacean zooplankton from the eastern Gulf of Mexico
Morris, M. J., & Hopkins, T. L. (1983) · Journal of Experimental Marine Biology and Ecology · doi:10.1016/0022-0981(83)90169-7 · Cited by 45

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks