Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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“The total lipids were determined according to Bligh and Dyer method [36], modified by Pereira [37].”
Method
Effect of steaming on chemical composition of Mediterranean mussel ( Mytilus galloprovincialis ): Evaluation of potential risk associated with human consumption
Peycheva, K., Panayotova, V., Stancheva, R., et al. (2022) · Food Science & Nutrition · doi:10.1002/fsn3.2903 · Cited by 26
“Total lipids (TL) were extracted from M. galloprovincialis using Bligh and Dyer procedure (Bligh & Dyer, 1959).”
Method
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“The Bligh-dyer method was used for total lipid extraction in each seaweed sample using a single-phase, with minor modifications [28].”
Method
Maternal stress in the postpartum period is associated with altered human milk fatty acid composition
Juncker, H. G., Naninck, E. F. G., Schipper, L., Lucassen, P. J., van Goudoever, J. B., de Rooij, S. R., & Kőrösi, A. (2022) · Clinical Nutrition · doi:10.1016/j.clnu.2022.09.013 · Cited by 27
“FAs were extracted according to Bligh and Dyer [42], adding dichloromethane, methanol and water.”
Method
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“Lipids were extracted using a solvent mixture of chloroform methanol-distilled water (50:100:50, v/v) according to the method of Bligh and Dyer [14].”
Method
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Passoth, V., Brandenburg, J., Chmielarz, M., Martín‐Hernández, G. C., Nagaraj, Y. N., Müller, B., & Blomqvist, J. (2023) · Yeast · doi:10.1002/yea.3838 · Cited by 34
“Probably the most commonly used methods for lipid quantification in oleaginous yeasts are based on the Folch or Bligh and Dyer extraction methods (Bligh & Dyer, 1959; Folch et al., 1957).”
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“Total lipids from the mouse's brain were extracted according to the chloroform/methanol/water method described previously [66].”
Method
Effect of replacement of soybean oil by Hermetia illucens fat on performance, digestibility, cecal microbiome, liver transcriptome and liver and plasma lipidomes of broilers
Schäfer, L., Grundmann, S. M., Maheshwari, G., Höring, M., Liebisch, G., Most, E., Eder, K., & Ringseis, R. (2023) · Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · doi:10.1186/s40104-023-00831-6 · Cited by 32
“An amount of 2 mg liver (wet weight) or a volume of 10 μL plasma was subjected to lipid extraction according to the protocol by Bligh and Dyer [52].”
MethodInfluential
Computational design and molecular dynamics simulations suggest the mode of substrate binding in ceramide synthases
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“5% (w/v) NaCl solution (1:1) was added to the flask and stirred for additional 1 h at room temperature (Bligh and Dyer 1959).”
Method
Key Biochemical Attributes to Assess Soil Ecosystem Sustainability
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Adam, I. K. U., Miltner, A., & Kästner, M. (2015) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-015-6822-8 · Cited by 26
2,5‐Dihydroxybenzoic acid salts for matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometric lipid analysis: Simplified spectra interpretation and insights into gas‐phase fragmentation
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Radić, I. D., Carić, M., Najdek, M., et al. (2014) · Mediterranean Marine Science · doi:10.12681/mms.436 · Cited by 26
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Zhou, C., Place, A. R., Yan, X., Xu, J., Luo, Q., William, E., & Jiang, Y. (2015) · Harmful Algae · doi:10.1016/j.hal.2015.08.004 · Cited by 27
The yeast Saccharomyces cerevisiae Pdr16p restricts changes in ergosterol biosynthesis caused by the presence of azole antifungals
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RETARDATION OF LIPID OXIDATION IN FISH OIL-ENRICHED FISH PÂTÉ- COMBINATION EFFECTS
Nielsen, N. S., & Jacobsen, C. (2011) · Journal of Food Biochemistry · doi:10.1111/j.1745-4514.2011.00605.x · Cited by 26
Supercritical Fluid Extraction and Chromatography of Lipids in Bilberry
Jumaah, F., Sandahl, M., & Turner, C. (2015) · Journal of the American Oil Chemists Society · doi:10.1007/s11746-015-2680-x · Cited by 26
Dietary inorganic phosphorus or microbial phytase supplementation improves growth, nutrient utilization and phosphorus mineralization of juvenile red sea bream, Pagrus major, fed soybean-based diets
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Landim, A. V., Cardoso, M. T. M., Castanheira, M., Fíoravantí, M. C. S., Louvandini, H., & McManus, C. (2011) · Tropical Animal Health and Production · doi:10.1007/s11250-011-9842-0 · Cited by 26
The content of docosahexaenoic acid in serum phospholipid is inversely correlated with plasma homocysteine levels in patients with end-stage renal disease
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Light Intensity Regulates LC-PUFA Incorporation into Lipids of Pavlova lutheri and the Final Desaturase and Elongase Activities Involved in Their Biosynthesis
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Potential role of acid ceramidase in conversion of cytostatic to cytotoxic end-point in pancreatic cancer cells
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Flight and substrate utilisation in laboratory-reared males of Atta sexdens
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Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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