Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
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Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Mosso, A. L., LeBlanc, J. G., Motta, C., et al. (2020) · LWT · doi:10.1016/j.lwt.2020.109339 · Cited by 25
The application of DFRC method for the analysis of carbohydrates in a peat bog: Validation and comparison with conventional chemical and thermochemical degradation techniques
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Bioavailability of polycyclic aromatic compounds (PACs) to the Sydney rock oyster (Saccostrea glomerata) from sediment matrices of an economically important Australian estuary
Idowu, O., Tran, T. K. A., Baker, P., et al. (2020) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2020.139574 · Cited by 25
A prospective, randomized, double blind, placebo-controlled evaluation of the effects of an n-3 essential fatty acids supplement (Agepi® ω3) on clinical signs, and fatty acid concentrations in the erythrocyte membrane, hair shafts and skin surface of dogs with poor quality coats
Combarros, D., Castilla‐Castaño, E., Lecru, L., Pressanti, C., Amalric, N., & Cadiergues, M. (2020) · Prostaglandins Leukotrienes and Essential Fatty Acids · doi:10.1016/j.plefa.2020.102140 · Cited by 25
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“The extraction of the total lipids of the cell and of the plasma membrane was performed using a slightly modified Bligh–Dyer method [44].”
Method
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Baldissera, M. D., Souza, C. F., Barroso, D. C., Falk, R. B., Wagner, R. C., Baldisserotto, B., & Val, A. L. (2020) · Fish Physiology and Biochemistry · doi:10.1007/s10695-020-00835-3 · Cited by 24
“Fillet fatty acid profile Lipids were extracted using the Bligh and Dyer (1959) method with adaptations according to Baldissera et al. (2020), and fatty acid methyl esters (FAMEs) were determined according to Hartman and Lago (1973).”
Method
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Metabolic Signatures of Tumor Responses to Doxorubicin Elucidated by Metabolic Profiling in Ovo
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“The tumor samples were extracted using the optimized Bligh−Dyer extraction method deploying methanol/water/dichloromethane as an extraction solvent [47].”
Method
Dendrogenin A Synergizes with Cytarabine to Kill Acute Myeloid Leukemia Cells In Vitro and In Vivo
Serhan, N., Mouchel, P., de Médina, P., Segala, G., Mougel, A., Saland, E., Rives, A., Lamazière, A., Després, G., Sarry, J., et al. (2020) · Cancers · doi:10.3390/cancers12071725 · Cited by 30
“Brieﬂy, after the addition of an internal standard of PE, total lipids were extracted from cells according to Bligh and Dyer [29].”
The Effect of Gelatine Packaging Film Containing a Spirulina platensis Protein Concentrate on Atlantic Mackerel Shelf Life
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Coating effect of rosemary extract combined with chitosan on storage quality of mori (Cirrhinus mrigala)
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Towards a Valorization of Corn Bioethanol Side Streams: Chemical Characterization of Post Fermentation Corn Oil and Thin Stillage
Lena, G. D., Ondrejíčková, P., del Pulgar, J. S., Cyprichová, V., Ježovič, T., Lucarini, M., Lombardi‐Boccia, G., Nicoli, S. F., Gabrielli, P., Aguzzi, A., et al. (2020) · Molecules · doi:10.3390/molecules25153549 · Cited by 25
“Total lipids were extracted from lyophilized thin stillage samples according to the method of Bligh and Dyer [36].”
Method
High Resolution Proteome of Lipid Droplets Isolated from the Pennate Diatom Phaeodactylum tricornutum (Bacillariophyceae) Strain pt4 provides mechanistic insights into complex intracellular coordination during nitrogen deprivation
Leyland, B., Zarka, A., Didi‐Cohen, S., Boussiba, S., & Khozin‐Goldberg, I. (2020) · Journal of Phycology · doi:10.1111/jpy.13063 · Cited by 27
“…protein removed, the remaining acetone containing the total LD lipids was evaporated to a minimal volume under vacuum using a rotary evaporator at 30°C, and then the residue was extracted with chloroform-methanol-water (1:1:0.8, v/v/v), according to the Bligh and Dyer method (Bligh and Dyer 1959).”
MethodInfluential
Simultaneous green extraction of fat and bioactive compounds of cocoa shell and protein fraction functionalities evaluation
Soares, I. D., Okiyama, D. C. G., & Rodrigues, C. E. D. C. (2020) · Food Research International · doi:10.1016/j.foodres.2020.109622 · Cited by 29
Effect of Nutrient Enrichment and Turbidity on Interactions Between Microphytobenthos and a Key Bivalve: Implications for Higher Trophic Levels
Hope, J. A., Hewitt, J. E., Pilditch, C. A., Savage, C., & Thrush, S. F. (2020) · Frontiers in Marine Science · doi:10.3389/fmars.2020.00695 · Cited by 28
Model infant formulas: Influence of types of whey proteins and oil composition on emulsion and powder properties
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Bamboo fiber improves the functional properties of reduced salt and phosphate‐free Bologna sausage
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Neutral Lipids, Glycolipids, and Phospholipids, Isolated from Sandfish (Arctoscopus japonicus) Eggs, Exhibit Anti-Inflammatory Activity in LPS-Stimulated RAW264.7 Cells through NF-κB and MAPKs Pathways
Rod–in, W., Monmai, C., Shin, I.-S., You, S., & Park, W. J. (2020) · Marine Drugs · doi:10.3390/md18090480 · Cited by 27
“The total lipids of Arctoscopus japonicus eggs were extracted from a freeze-dried powder by using the Bligh and Dyer method (1959) [62].”
Method
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Massé, A., Tribollet, A. D., Meziane, T., Bourguet‐Kondracki, M., Yéprémian, C., Sève, C., Thiney, N., Longeon, A., Couté, A., & Domart-Coulon, I. J. (2020) · Environmental Microbiology · doi:10.1111/1462-2920.15256 · Cited by 24
Yeast fermentation towards biodiesel: Maximizing resource recovery by integrating with biohydrogen production in biorefinery framework
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Sustainable Management of Secondary Raw Materials from the Marine Food-Chain: A Case-Study Perspective
Lucarini, M., Zuorro, A., Lena, G. D., Lavecchia, R., Durazzo, A., Benedetti, B., & Lombardi‐Boccia, G. (2020) · Sustainability · doi:10.3390/su12218997 · Cited by 25
Sample Preparation Methods for Lipidomics Approaches Used in Studies of Obesity
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Cerebrospinal Fluid Profiles in Parkinson’s Disease: No Accumulation of Glucosylceramide, but Significant Downregulation of Active Complement C5 Fragment
Niimi, Y., Mizutani, Y., Akiyama, H., Watanabe, H., Shiroki, R., Hirabayashi, Y., Hoshinaga, K., & Mutoh, T. (2020) · Journal of Parkinson s Disease · doi:10.3233/jpd-202310 · Cited by 26
“Lipids were extracted from CSF according to the method of Bligh and Dyer [25].”

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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