Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Dong, H., Liu, W., Zhang, H., Wang, Z., Feng, F., Zhou, L., Duan, H., Xu, T., Li, X., & Ma, J. (2023) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-023-02855-8 · Cited by 23
“Chloroform–methanol method was used to extract lipid [22].”
Method
Evaluation of Lipid Extracts from the Marine Fungi Emericellopsis cladophorae and Zalerion maritima as a Source of Anti-Inflammatory, Antioxidant and Antibacterial Compounds
Abraúl, M., Alves, A., Hilário, S., Melo, T., Conde, T., Domingues, M. R., & Rey, F. (2023) · Marine Drugs · doi:10.3390/md21040199 · Cited by 22
“The total lipids were extracted from the mycelium using the Bligh and Dyer method [59] with some modifications.”
Method
Untargeted Lipidomic Profiling Reveals Lysophosphatidylcholine and Ceramide as Atherosclerotic Risk Factors in apolipoprotein E Knockout Mice
Law, S., Chan, H., Ke, G., Kamatam, S., Marathe, G. K., Ponnusamy, V. K., & Ke, L. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24086956 · Cited by 22
“Plasma lipids were extracted using the Folch technique [58,59].”
Method
Drug Repurposing Flubendazole to Suppress Tumorigenicity via PCSK9-dependent Inhibition and Potentiate Lenvatinib Therapy for Hepatocellular Carcinoma
Jin, W., Yu, J., Su, Y., Lin, H., Liu, T., Chen, J., Ge, C., Zhao, F., Geng, Q., Mao, L., et al. (2023) · International Journal of Biological Sciences · doi:10.7150/ijbs.81415 · Cited by 20
“Cholesterol extraction was performed as described before [24].”
Method
Biochemical composition of Hediste diversicolor (OF Müller, 1776) (Annelida: Nereidae) reared on different types of aquaculture sludge
Anglade, I., Dahl, T. H., Kristensen, B. S. B., et al. (2023) · Frontiers in Marine Science · doi:10.3389/fmars.2023.1197052 · Cited by 20
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Dhavamani, S., Park, J. C., McAnany, J. J., Kim, T.-H., Ma, G., Yao, X., Antharavally, B. S., Oroskar, A. R., Oroskar, A. A., Layden, B. T., et al. (2023) · Scientific Reports · doi:10.1038/s41598-023-36268-0 · Cited by 20
Extraction of Nannochloropsis Fatty Acids Using Different Green Technologies: The Current Path
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Physicochemical and functional properties of rainbow trout (Oncorhynchus mykiss) hydrolysate
Kvangarsnes, K., Daukšas, E., Tolstorebrov, I., Rustad, T., Bartolomei, M., Xu, R., Lammi, C., & Cropotova, J. (2023) · Heliyon · doi:10.1016/j.heliyon.2023.e17979 · Cited by 24
“The extraction of lipids was performed according to the method of Bligh and Dyer [14].”
Method
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Ennas, C., Pasquini, V., Abyaba, H., Addis, P., Sarà, G., & Pusceddu, A. (2023) · Scientific Reports · doi:10.1038/s41598-023-38543-6 · Cited by 24
The alternative bile acid pathway can predict food allergy persistence in early childhood
Lee, S., Park, Y. M., Yoo, H. J., Lee, S., Choi, E. J., Paek, E. Y., Song, K. B., Yoon, J., & Hong, S. (2023) · Pediatric Allergy and Immunology · doi:10.1111/pai.14003 · Cited by 18
Development of yeast and microalgae consortium biofilm growth system for biofuel production
Bisht, B., Verma, M., Sharma, R., Chauhan, P. K., Pant, K., Kim, H., Vlaskin, M. S., & Kumar, V. (2023) · Heliyon · doi:10.1016/j.heliyon.2023.e19353 · Cited by 21
“The lyophilized biomass pellets were further employed for lipid analysis using gravimetric methods [22].”
Method
Staphylococcus aureus delta toxin modulates both extracellular membrane vesicle biogenesis and amyloid formation
Wang, X., Uppu, D. S. S. M., Dickey, S. W., Burgin, D. J., Otto, M. J., & Lee, J. C. (2023) · mBio · doi:10.1128/mbio.01748-23 · Cited by 24
Cationic amphiphilic drugs induce accumulation of cytolytic lysoglycerophospholipids in the lysosomes of cancer cells and block their recycling into common membrane glycerophospholipids
Nielsen, I. Ø., Clemmensen, K. K. B., Fogde, D. L., Dietrich, T. N., Giacobini, J. D., Bilgin, M., Jäättelä, M., & Maeda, K. (2023) · Molecular Biology of the Cell · doi:10.1091/mbc.e23-06-0263 · Cited by 16
“Total lipids were extracted from 0.2 × 10 6 cells or purified lysosomes in suspension in 200 µl 155 mM ammonium bicarbonate using a modified Bligh and Dyer protocol (Bligh and Dyer, 1959).”
Influential
Bioprospecting of Ten Microalgae Species Isolated from Saline Water Lake for Evaluation of the Biodiesel Production
Khosravinia, S., Malekzadeh-Shafaroudi, S., Bagheri, A., Behdad, A., & Moshtaghi, N. (2023) · BioEnergy Research · doi:10.1007/s12155-023-10707-2 · Cited by 23
“Total lipid was extracted from approximately 100 mg of microalgae powder using a modified method of Bligh and Dyer [17].”
Investigating the Endophyte Actinomycetota sp. JW0824 Strain as a Potential Bioinoculant to Enhance the Yield, Nutritive Value, and Chemical Composition of Different Cultivars of Anise (Pimpinella anisum L.) Seeds
Mahmoud, A. M., Reyad, A. M., Khalaf, M. H., et al. (2024) · Biology · doi:10.3390/biology13080553 · Cited by 20
“The methodology described by Wang et al. [34] was used to determine the EO-related precursors, i.e., phenylalanine, cinnamic acid, and shikimic acid.”
Method
Step-wise evolution of azole resistance through copy number variation followed by KSR1 loss of heterozygosity in Candida albicans
Zande, P. V., Gautier, C., Kawar, N., Maufrais, C., Metzner, K., Wash, E., Beach, A. K., Bracken, R., Maciel, E. I., de Sá, N. P., et al. (2024) · PLoS Pathogens · doi:10.1371/journal.ppat.1012497 · Cited by 21
Development of vegetative oil sorghum: From lab‐to‐field
Park, K., Quach, T. N., Clark, T. J., et al. (2024) · Plant Biotechnology Journal · doi:10.1111/pbi.14527 · Cited by 20
Enhancement of lipid production in marine microalga Tetraselmis sp. through salinity variation
Kim, G., Lee, C., & Lee, K. (2015) · Korean Journal of Chemical Engineering · doi:10.1007/s11814-015-0089-8 · Cited by 20
Oil Extraction from Algae: A Comparative Approach
Valizadeh Derakhshan, M., Nasernejad, B., Abbaspour‐Aghdam, F., & Hamidi, M. (2015) · Biotechnology and Applied Biochemistry · doi:10.1002/bab.1270 · Cited by 20
Cocoa and cocoa flavanol epicatechin improve hepatic lipid metabolism in in vivo and in vitro models. Role of PKCζ
Cordero‐Herrera, I., Martín, M. Á., Fernández‐Millán, E., Álvarez, C., Goya, L., & Ramos, S. (2015) · Journal of Functional Foods · doi:10.1016/j.jff.2015.06.033 · Cited by 20
Hydrophilic interaction liquid chromatography–electrospray ionization-tandem mass spectrometry of a complex mixture of native and oxidized phospholipids
Losito, I., Facchini, L., Diomede, S., et al. (2015) · Journal of Chromatography A · doi:10.1016/j.chroma.2015.10.023 · Cited by 20
The Utilization of the Acyl‐CoA and the Involvement PDAT and DGAT in the Biosynthesis of Erucic Acid‐Rich Triacylglycerols in Crambe Seed Oil
Furmanek, T., Demski, K., Banaś, W., et al. (2014) · Lipids · doi:10.1007/s11745-014-3886-7 · Cited by 20
Fatty acid composition of Oncorhynchus mykiss during embryogenesis and other developmental stages
Zengi̇n, H., & Akpınar, M. A. (2006) · Biologia · doi:10.2478/s11756-006-0056-2 · Cited by 20
Above- and below-ground interactions with agricultural management: Effects of soil microbial communities on barley and aphids
Williams, A., Birkhofer, K., & Hedlund, K. (2014) · Pedobiologia · doi:10.1016/j.pedobi.2014.01.004 · Cited by 20
High intraspecific variability in the diet of a deep-sea nematode: Stable isotope and fatty acid analyses of Deontostoma tridentum on Chatham Rise, Southwest Pacific
Leduc, D., Brown, J. C. S., Bury, S. J., et al. (2014) · Deep Sea Research Part I Oceanographic Research Papers · doi:10.1016/j.dsr.2014.11.002 · Cited by 20
Impaired liver regeneration in Ldlr−/− mice is associated with an altered hepatic profile of cytokines, growth factors, and lipids
Pauta, M., Rotllán, N., Vales, F., Fernández‐Hernando, A., Allen, R. M., Ford, D. A., Marı́, M., Jiménez, W., Baldán, Á., Morales‐Ruiz, M., et al. (2013) · Journal of Hepatology · doi:10.1016/j.jhep.2013.05.026 · Cited by 20
Effects of acclimation temperature on enzymatic capacities and mitochondrial membranes from the body wall of the earthworm Lumbricus terrestris
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Effect of the Nutritional Status of Semi-continuous Microalgal Cultures on the Productivity and Biochemical Composition of Brachionus plicatilis
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Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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