Oil Extraction from Algae: A Comparative Approach
Abstract
In this article, various methods including soxhlet, Bligh & Dyer (B&D), and ultrasonic-assisted B&D were investigated for the extraction of lipid from algal species Chlorella vulgaris. Relative polarity/water content and impolar per polar ratios of solvents were considered to optimize the relative proportions of each triplicate agent by applying the response surface method (RSM). It was found that for soxhlet, hexane-methanol (54-46%, respectively) with total lipid extraction of 14.65% and chloroform-methanol (54-46%, respectively) with the extraction of 19.87% lipid were the best set of triplicate where further addition of acetone to the first group and ethanol to the second group did not contributed to further extraction. In B&D, however, chloroform-methanol-water (50%-35%-15%, respectively) reached the all-time maximum of 24%. Osmotic shock as well as ultrasonication contributed to 3.52% of further extraction, which is considered to promote the total yield up to almost 15%. From the growth data and fatty acid analysis, the applied method was assessed to be appropriate for biodiesel production with regard to selectivity and extraction yield.
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Conventional solvent extraction methods such as hexane extraction or the Bligh and Dyer method are widely used for their efficiency in extracting microalgal lipids.
“Conventional solvent extraction methods, such as hexane extraction [110] or the Bligh and Dyer method [111], are widely used for their efficiency in extracting lipids.”
From Algal-Based Carbonaceous Materials for Environmental Remediation: Advances in Wastewater Treatment, Carbon Sequestration, and Biofuel Applications (2025) · cites this paper as [110]
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González Fernández, L. A., Medellín Castillo, N. A., Sánchez Polo, M., Navarro Frómeta, A. E., & Vilasó Cadre, J. E. (2025) · Processes · doi:10.3390/pr13020556 · Cited by 37 · see citing works
- Authors
- Valizadeh Derakhshan, M., Nasernejad, B., Abbaspour‐Aghdam, F., & Hamidi, M.
- Affiliations
- Department of Chemical Engineering Amirkabir University of Technology (Tehran Polytechnic) Tehran Iran
- Amirkabir University of Technology (IR)
- Sahand University of Technology (IR)
- ORCID
- Nasernejad, Bahram
- Venue
- Biotechnology and Applied Biochemistry
- Publisher
- Wiley
- Published
- 2014-11-24
- Volume
- 62
- Issue
- 3
- Pages
- 375-382
- ISSN
- 0885-4513 (ISSN-L)
- 1470-8744
- OpenAlex
- W1500154665
- Cited by
- 20
- References
- 30
- Citations by year
- 2025: 1 · 2022: 2 · 2021: 5 · 2020: 4 · 2018: 2 · 2017: 4 · 2016: 2
- FWCI
- 0.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology and Applied Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Engineering › Biomedical Engineering › Biodiesel Production and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- Chlorella vulgaris, Bligh and Dyer method, ultrasound-assisted extraction, Soxhlet extraction, solvent optimization, osmotic shock, fatty acid composition, biodiesel feedstock, extraction yield
- MeSH terms
- Chemical Fractionation, Plant Oils, Sonication, Chlorella vulgaris, Biofuels
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