Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Egeler, O., & Williams, T. D. (2000) · The Auk · doi:10.1093/auk/117.1.110 · Cited by 14
Dietary olive oil intake aggravates psoriatic skin inflammation in mice via Nrf2 activation and polyunsaturated fatty acid imbalance
Donato‐Trancoso, A., Atella, G. C., & Romana‐Souza, B. (2022) · International Immunopharmacology · doi:10.1016/j.intimp.2022.108851 · Cited by 15
Effect of dietary β-mannanase supplementation on growth performance, digestibility, and gene expression levels of Cyprinus carpio (Linnaeus) fingerlings fed a plant protein-rich diet
Dawood, A., & Shi, W. (2022) · Frontiers in Veterinary Science · doi:10.3389/fvets.2022.956054 · Cited by 14
“The microKjeldahl apparatus and the petroleum ether extraction method by Soxhlet apparatus (26) were used to estimate crude protein (N× 6.”
Method
Specific Lipid Studies in Complex Membranes by Solid‐State NMR Spectroscopy
van Beekveld, R. A. M., Derks, M. G. N., Kumar, R., Smid, L., Maaß, T., Medeiros‐Silva, J., Breukink, E. J., & Weingarth, M. H. (2022) · Chemistry - A European Journal · doi:10.1002/chem.202202472 · Cited by 14
Lipid nanoparticles for antisense oligonucleotide gene interference into brain border-associated macrophages
Calero, M., Moleiro, L. H., Sayd, A., Dorca, Y., Miquel-Rio, L., Paz, V., Robledo‐Montaña, J., Enciso, E., Acción, F., Herráez‐Aguilar, D., et al. (2022) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2022.887678 · Cited by 14
“Electrostatics and hydrophobicity are both counterbalanced by tuning BD permittivity, hence resulting in catanionic GR:DChol2 lipoplexes stoichiometrically formed upon a thermodynamic philic–phobic trade-off (Bligh and Dyer, 1959).”
BackgroundMethod
Maternal omega-3 fatty acid deficiency affects fetal thermogenic development and postnatal musculoskeletal growth in mice
Vilasagaram, S., Molangiri, A., Varma, S., Mallepogu, A., Kona, S. R., Ibrahim, A., Duttaroy, A. K., & Basak, S. (2022) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2022.109218 · Cited by 14
Interaction among dietary n‐3 and n‐6 polyunsaturated fatty acid intake, fatty acid desaturase 2 genetic variants, and low‐density lipoprotein cholesterol levels in type 2 diabetes patients
Huang, P., Cheng, H., Su, Y., Huang, M., Hsu, C., Hwang, S., Shin, S., & Chang, W. (2022) · Journal of Diabetes Investigation · doi:10.1111/jdi.13944 · Cited by 15
Multivariable optimization process of heterotrophic growth of Chlorella vulgaris
Geada, P., Francisco, D., Pereira, F., Maciel, F., Madureira, L., Barros, A. I. R. N. A., Silva, J., Vicente, A. A., & Teixeira, J. A. (2022) · Food and Bioproducts Processing · doi:10.1016/j.fbp.2022.12.004 · Cited by 14
Barley β-glucan inhibits digestion of soybean oil in vitro and lipid-lowering effects of digested products in cell co-culture model
Li, J., Zhou, Y., Zhang, J., Cui, L., Lu, H., Zhu, Y. G., Zhao, Y., Fan, S., & Xiao, X. (2022) · Food Research International · doi:10.1016/j.foodres.2022.112378 · Cited by 14
Successful acclimation of marine diatoms Chaetoceros curvisetus / pseudocurvisetus to climate change
Vrana, I., Gašparović, B., Geček, S., Godrijan, J., Novak, T., Kazazić, S., Mlakar, ⎜., Kužat, N., Pfannkuchen, M. A., & Pfannkuchen, D. M. (2023) · Limnology and Oceanography · doi:10.1002/lno.12293 · Cited by 14
“Particulate lipid extraction was performed using a modified method of Bligh and Dyer (1959).”
Method
Microalgal cultivation characteristics using commercially available air-cushion packaging material as a photobioreactor
Merz, C. R., Arora, N., Welch, M., Lo, E., & Philippidis, G. P. (2023) · Scientific Reports · doi:10.1038/s41598-023-30080-6 · Cited by 15
Physicochemical Quality and Fatty Acid Profile in the Meat of Goats Fed Forage Cactus as a Substitute for Tifton 85 Hay
Pinheiro, R. S. B., de Farias, I. M. S. C., Francisco, C. D. L., et al. (2023) · Animals · doi:10.3390/ani13060957 · Cited by 14
“Lipid extraction was performed using the cold technique with 20 g of meat and a methanol: chloroform: water solution [33].”
Method
A Streptococcus pyogenes DegV protein regulates the membrane lipid content and limits the formation of extracellular vesicles
Lambert, C., Poullion, T., Zhang, Q., Schmitt, A., Massé, J., Gloux, K., Poyart, C., & Fouet, A. (2023) · PLoS ONE · doi:10.1371/journal.pone.0284402 · Cited by 12
“Lipid extractions were performed as previously described [5, 33, 34].”
Method
Distinct domains in Ndc1 mediate its interaction with the Nup84 complex and the nuclear membrane
Amm, I., Weberruss, M. H., Hellwig, A., Schwarz, J., Tatarek‐Nossol, M., Lüchtenborg, C., Kallas, M., Brügger, B., Hurt, E., & Antonin, W. (2023) · The Journal of Cell Biology · doi:10.1083/jcb.202210059 · Cited by 14
“Lipid extracts were prepared in presence of internal lipid standards via acidic Bligh-Dyer lipid extraction (Bligh and Dyer, 1959).”
Impact of FADS gene variation and dietary fatty acid exposure on biochemical and anthropomorphic phenotypes in a Hispanic/Latino cohort
Sergeant, S., Keith, B., Seeds, M. C., Legins, J. A., Young, C. B., Vitolins, M. Z., & Chilton, F. H. (2023) · Frontiers in Nutrition · doi:10.3389/fnut.2023.1111624 · Cited by 14
“A total lipid extract was prepared from RBC lysates (400 μl in duplicate) using an acidified Bligh-Dyer method (21).”
Method
A review on pretreatment methods for lipid extraction from microalgae biomass
Husin, M. A. M., Yasin, N. H. M., Takriff, M. S., & Jamar, N. H. (2023) · Preparative Biochemistry & Biotechnology · doi:10.1080/10826068.2023.2214923 · Cited by 16
“Bligh and Dyer ’ s [67] method, Folch ’ s [91] method, and Hara and Radin ’ s [93] method were developed to extract lipids from animal tissues.”
MethodInfluential
Progress of Mass Spectrometry-Based Lipidomics in the Dairy Field
Ren, W., Sun, M., Shi, X., Wang, T., Wang, Y., Wang, C., & Li, M. (2023) · Foods · doi:10.3390/foods12112098 · Cited by 14
A rapid single-phase extraction for polar staphylococcal lipids
Bimpeh, K., & Hines, K. M. (2023) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-023-04758-9 · Cited by 13
PLAAT1 deficiency alleviates high‐fat diet‐induced hepatic lipid accumulation in mice
Rahman, S., Sasaki, S., Uyama, T., Hussain, Z., Sikder, M. M., Saiga, H., Ohmura‐Hoshino, M., Ohta, K., Miki, Y., Hoshino, K., et al. (2023) · The FASEB Journal · doi:10.1096/fj.202201033r · Cited by 15

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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