Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 30,601–30,700 of 48,846

Hagfish slime exudate stabilization and its effect on slime formation and functionality
Böni, L., Zurflüh, R., Widmer, M., et al. (2017) · Biology Open · doi:10.1242/bio.025528 · Cited by 14
Combining Time-of-Flight Secondary Ion Mass Spectrometry Imaging Mass Spectrometry and CARS Microspectroscopy Reveals Lipid Patterns Reminiscent of Gene Expression Patterns in the Wing Imaginal Disc of Drosophila melanogaster
Marty, F., Rago, G., Smith, D. F., et al. (2017) · Analytical Chemistry · doi:10.1021/acs.analchem.7b00125 · Cited by 14
Brassinosteroid improves lipid productivity and stress tolerance of Chlorella cells induced by high temperature
Liu, J., Qiu, W., & Xia, D. (2017) · Journal of Applied Phycology · doi:10.1007/s10811-017-1223-2 · Cited by 16
Chemical-physical characteristics of buffalo (Bubalus bubalis) meat subjected to different aging times
da Luz, P. A. C., Jorge, A. M., Francisco, C. D. L., et al. (2017) · Acta Scientiarum Animal Sciences · doi:10.4025/actascianimsci.v39i4.36799 · Cited by 14
Paracoccus sordidisoli sp. nov., isolated from an agricultural field contaminated with hexachlorocyclohexane isomers
Singh, A. K., Kohli, P., Mahato, N. K., et al. (2017) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.002289 · Cited by 14
The potential of identifying replacement gilts by screening for lipid biomarkers in reproductive tract swabs taken at weaning
Casey, T., Harlow, K., Ferreira, C. R., Sobreira, T. J. P., Schinckel, A. P., & Stewart, K. R. (2017) · Journal of Applied Animal Research · doi:10.1080/09712119.2017.1384733 · Cited by 13
Resveratrol suppresses ethanol stress in winery and bottom brewery yeast by affecting superoxide dismutase, lipid peroxidation and fatty acid profile
Gharwalová, L., Sigler, K., Dolezalova, J., Masák, J., Řezanka, T., & Kolouchová, I. (2017) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-017-2371-x · Cited by 14
Chemical genomic guided engineering of gamma-valerolactone tolerant yeast
Bottoms, S., Dickinson, Q., McGee, M. A., Hinchman, L., Higbee, A. J., Hebert, A. S., Serate, J., Xie, D., Zhang, Y., Coon, J. J., et al. (2018) · Microbial Cell Factories · doi:10.1186/s12934-017-0848-9 · Cited by 14
Bioenergetic reprogramming plasticity under nitrogen depletion by the unicellular green alga Scenedesmus obliquus
Papazi, A., Korelidou, A., Andronis, E. A., Parasyri, A., Stamatis, N., & Kotzabasis, K. (2017) · Planta · doi:10.1007/s00425-017-2816-3 · Cited by 14
Maternal protein restriction during lactation induces early and lasting plasma metabolomic and hepatic lipidomic signatures of the offspring in a rodent programming model
Agnoux, A. M., Ghaziri, A. E., Moyon, T., Pagniez, A., David-Sochard, A., Simard, G., Parnet, P., Qannari, E. M., Darmaun, D., Antignac, J., et al. (2017) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2017.11.009 · Cited by 15
Quality attributes of French fries as affected by different coatings, frozen storage and frying conditions
Kizito, K. F., ABDEL-AAL, M. H., Mh, R., et al. (2017) · Journal of Agricultural Science and Botany · doi:10.35841/2591-7897.1.1.18-24 · Cited by 14
Allene oxide synthase, allene oxide cyclase and jasmonic acid levels in Lotus japonicus nodules
Zdyb, A., Salgado, M. G., Demchenko, K. N., et al. (2018) · PLoS ONE · doi:10.1371/journal.pone.0190884 · Cited by 14
Alfalfa but not milk in lamb's diet improves meat fatty acid profile and α-tocopherol content
Rodríguez, J. Á., Ripoll, G., Lobón, S., et al. (2018) · Food Research International · doi:10.1016/j.foodres.2018.03.007 · Cited by 14
Metabolic profiling and quality assessment during the postharvest of two tangor varieties subjected to heat treatments
Moreno, A. S., Perotti, V., Margarit, E., et al. (2018) · Postharvest Biology and Technology · doi:10.1016/j.postharvbio.2018.03.014 · Cited by 14
Role of dietary α- and γ-tocopherol from Rosa mosqueta oil in the prevention of alterations induced by high-fat diet in a murine model
Tapia, G., Silva, D., Romero, N., Pettinelli, P., Dossi, C. G., de Miguel, M., & Gonzalez‐Mañán, D. (2018) · Nutrition · doi:10.1016/j.nut.2018.01.012 · Cited by 14
Performance and meat quality of Nellore bulls fed crude glycerin combined with soybean oil
Fiorentini, G., Messana, J. D., Neto, A. J., Sgobi, E. G., Castagnino, P. D. S., & Berchielli, T. T. (2018) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2018.04.013 · Cited by 15
Transgenic overexpression of CTRP3 prevents alcohol-induced hepatic triglyceride accumulation
Trogen, G. H., Bacon, J., Li, Y., Wright, G. L., DeGroat, A. R., Hagood, K. L., Warren, Z. C., Forsman, A. D., Kilaru, A., Clark, W. A., et al. (2018) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00050.2018 · Cited by 14
Evaluation of the turmeric dye extraction residue in the formation of protective coating on fresh bananas (Musa acuminata cv. ‘Maçã’)
de Paula, R. L., Maniglia, B. C., ASSIS, O. B. G., et al. (2018) · Journal of Food Science and Technology · doi:10.1007/s13197-018-3252-5 · Cited by 14
Dual Emitter Nano-Electrospray Ionization Coupled to Differential Ion Mobility Spectrometry-Mass Spectrometry for Shotgun Lipidomics
Keating, J. E., & Glish, G. L. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.8b01528 · Cited by 14
Black Raspberry Seed Oil Improves Lipid Metabolism by Inhibiting Lipogenesis and Promoting Fatty‐Acid Oxidation in High‐Fat Diet‐Induced Obese Mice and db / db Mice
Lee, H. J., Jung, H., Cho, H., Lee, K., & Hwang, K. T. (2018) · Lipids · doi:10.1002/lipd.12050 · Cited by 15
The association of lipophilic phospholipids with native bovine casein micelles in skim milk: Effect of lactation stage and casein micelle size
Cheema, M. K., Smith, P. B., Patterson, A. D., et al. (2018) · Journal of Dairy Science · doi:10.3168/jds.2017-14137 · Cited by 14
A novel role of endothelial autophagy as a regulator of myocardial fatty acid oxidation
Altamimi, T., Chowdhury, B., Singh, K. K., et al. (2018) · Journal of Thoracic and Cardiovascular Surgery · doi:10.1016/j.jtcvs.2018.07.047 · Cited by 14
Mouth gape determines the response of marine top predators to long-term fishery-induced changes in food web structure
Drago, M., Franco‐Trecu, V., Segura, A. M., Valdivia, M., González, E. M., Aguilar, Á., & Cardona, L. (2018) · Scientific Reports · doi:10.1038/s41598-018-34100-8 · Cited by 14
Could the growth of Litopenaeus vannamei be compensated by long-term cyclic feed deprivation and following refeeding?
Yildirim, M., & Aktaş, M. (2018) · Journal of Applied Aquaculture · doi:10.1080/10454438.2018.1536007 · Cited by 14
Biochemical characterization of Phaeodactylum tricornutum for microalgae-based biorefinery
Branco‐Vieira, M., Martin, S. S., Agurto-Muñoz, C., et al. (2018) · Energy Procedia · doi:10.1016/j.egypro.2018.10.079 · Cited by 14
A simplified protocol for fatty acid profiling of milk fat without lipid extraction
Liu, Z., Moate, P. J., & Rochfort, S. J. (2018) · International Dairy Journal · doi:10.1016/j.idairyj.2018.11.005 · Cited by 14
Oleander Stem and Root Standardized Extracts Mitigate Acute Hyperglycaemia by Limiting Systemic Oxidative Stress Response in Diabetic Mice
Dey, P., Saha, M. R., Choudhuri, S. R., et al. (2019) · Advances in Pharmacological Sciences · doi:10.1155/2019/7865359 · Cited by 14
Evaluation of Sample Preparation Methods for the Analysis of Reef-Building Corals Using 1H-NMR-Based Metabolomics
Andersson, E. R., Day, R. D., Loewenstein, J. M., et al. (2019) · Metabolites · doi:10.3390/metabo9020032 · Cited by 14
Lipidomics for wildlife disease etiology and biomarker discovery: a case study of pansteatitis outbreak in South Africa
Koelmel, J. P., Ulmer, C. Z., Fogelson, S. B., et al. (2019) · Metabolomics · doi:10.1007/s11306-019-1490-9 · Cited by 14
Fatty acid composition and nutritional profiles of Brycon spp. from central Amazonia by different methods of quantification
Petenuci, M. E., dos Santos, V. J., Gualda, I. P., Lopes, A. P., Schneider, V. V. A., Santos, O. O., & Visentainer, J. V. (2019) · Journal of Food Science and Technology · doi:10.1007/s13197-019-03654-4 · Cited by 15
Fish oil supplementation during adolescence attenuates metabolic programming of perinatal maternal high-fat diet in adult offspring
Souza, A. F. P., Souza, L. L., Oliveira, L. S. D., Cordeiro, A., Souza, E., Kluck, G. E. G., Atella, G. C., Trevenzoli, I. H., & Pazos-Moura, C. C. (2019) · British Journal Of Nutrition · doi:10.1017/s0007114519000771 · Cited by 14
Triacylglycerol Production in the Snow Algae Chlamydomonas nivalis under Different Nutrient Conditions
Liu, Y., & Nakamura, Y. (2019) · Lipids · doi:10.1002/lipd.12143 · Cited by 14
A novel synthesis of trans-unsaturated fatty acids by the Gram-positive commensal bacterium Enterococcus faecalis FA2-2
Kondakova, T., Kumar, S., & Cronan, J. E. (2019) · Chemistry and Physics of Lipids · doi:10.1016/j.chemphyslip.2019.04.010 · Cited by 14
Detergent-resistant microdomains (lipid rafts) in endomembranes of the wild halophytes
Розенцвет, О. А., Нестеркина, И. С., Озолина, Н. В., et al. (2019) · Functional Plant Biology · doi:10.1071/fp18263 · Cited by 14
IFN-α-2b treatment protects against diet-induced obesity and alleviates non-alcoholic fatty liver disease in mice
Quiroga, A. D., Comanzo, C. G., Heit-Barbini, F., Lucci, A., Vera, M. C., Lorenzetti, F., Ferretti, A. C., Ceballos, M. P., Álvarez, M. D. L., & Carrillo, M. C. (2019) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2019.114650 · Cited by 15
Repetitive extraction of botryococcene from Botryococcus braunii: a study of the effects of different solvents and operating conditions
Jackson, B. A., Bahri, P. A., & Moheimani, N. R. (2019) · Journal of Applied Phycology · doi:10.1007/s10811-019-01883-w · Cited by 16
Specific Wheat Fractions Influence Hepatic Fat Metabolism in Diet-Induced Obese Mice
Gräf, D., Weitkunat, K., Dötsch, A., Liebisch, G., Döring, M., Krüger, R., Stoll, D., Vatareck, E., von Coburg, E., Loh, G., et al. (2019) · Nutrients · doi:10.3390/nu11102348 · Cited by 14
Determination of the collision cross sections of cardiolipins and phospholipids from Pseudomonas aeruginosa by traveling wave ion mobility spectrometry-mass spectrometry using a novel correction strategy
Deschamps, E., Schmitz, I., Schaumann, A., Dé, E., Loutelier‐Bourhis, C., Alexandre, S., & Afonso, C. (2019) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-019-02194-2 · Cited by 18
Metabolomic profiles associated with subtypes of prediabetes among Mexican Americans in Starr County, Texas, USA
Jun, G., Aguilar, D. A., Evans, C. R., Burant, C., & Hanis, C. L. (2019) · Diabetologia · doi:10.1007/s00125-019-05031-4 · Cited by 14
Differences in the lipid patterns during maturation of 3T3-L1 adipocytes investigated by thin-layer chromatography, gas chromatography, and mass spectrometric approaches
Popkova, Y., Dannenberger, D., Schiller, J., et al. (2019) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-019-02243-w · Cited by 14
Enriching Rotifers with “Premium” Microalgae: Rhodomonas lens
Coutinho, P., Ferreira, M., Freire, I., & Otero, A. (2019) · Marine Biotechnology · doi:10.1007/s10126-019-09936-4 · Cited by 14
Plasma ceramide is increased and associated with proteinuria in women with pre-eclampsia and HELLP syndrome
Amraoui, F., Lahsinoui, H. H., Spijkers, L. J. A., et al. (2019) · Pregnancy Hypertension · doi:10.1016/j.preghy.2019.12.006 · Cited by 14
Membrane Binding, Cellular Cholesterol Content and Resealing Capacity Contribute to Epithelial Cell Damage Induced by Suilysin of Streptococcus suis
Vötsch, D., Willenborg, M., Oelemann, W. M. R., Brogden, G., & Valentin‐Weigand, P. (2019) · Pathogens · doi:10.3390/pathogens9010033 · Cited by 16
Biodiesel production from Egyptian isolate Fusarium oxysporum NRC2017
Sayeda, A. A., Mohsen, S. A., Osama, H. E. S., et al. (2019) · Bulletin of the National Research Centre/Bulletin of the National Research Center · doi:10.1186/s42269-019-0254-z · Cited by 14
Moderate intake of BCAA-rich protein improves glucose homeostasis in high-fat-fed mice
Roquetto, A. R., Moura, C. S., Santos-Junior, V. D. A., Oliveira, P. O. S., Machado, K. I. A., Carvalho, G. C. B. C., Risso, É. M., & Amaya‐Farfán, J. (2020) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2019.108332 · Cited by 14
A2E Distribution in RPE Granules in Human Eyes
Guan, Z., Li, Y., Jiao, S., Yeasmin, N., Rosenfeld, P. J., Dubovy, S. R., Lam, B. L., & Wen, R. (2020) · Molecules · doi:10.3390/molecules25061413 · Cited by 13
“Total lipids were extracted from samples by a modified Bligh and Dyer method [35,36].”
Method
Altered composition and functional profile of high-density lipoprotein in leprosy patients
Lemes, R. M. R., Silva, C. A. M., Marques, M. A. M., Atella, G. C., Nery, J. A. D. C., Nogueira, M. R. S., Rosa, P. S., Soares, C. T., De, P., Chatterjee, D., et al. (2020) · PLoS neglected tropical diseases · doi:10.1371/journal.pntd.0008138 · Cited by 14
Utilization of egg-laying hens (Gallus Gallus domesticus) for production of ingredients for human consumption and animal feed
Hjellnes, V., Šližytė, R., Rustad, T., et al. (2020) · BMC Biotechnology · doi:10.1186/s12896-020-00618-x · Cited by 14
Morphological and structural response of Bacillus sp. SFC 500‐1E after Cr(VI) and phenol treatment
Fernández, M., Paulucci, N. S., Reynoso, E., et al. (2020) · Journal of Basic Microbiology · doi:10.1002/jobm.202000076 · Cited by 14
Phospholipids modifications in human hepatoma cell lines (HepG2) exposed to silver and iron oxide nanoparticles
Adeyemi, J. A., Sorgi, C. A., Machado, A. R. T., Ogunjimi, A. T., Gardinassi, L. G., Nardini, V., Faccioli, L. H., Antunes, L. M. G., & Barbosa, F. (2020) · Archives of Toxicology · doi:10.1007/s00204-020-02789-0 · Cited by 13
Native fruits from southern Brazil: Physico‐chemical characterization, centesimal composition, and mineral content
Rockett, F. C., Schmidt, H. D. O., Pagno, C. H., et al. (2020) · Journal of Food Processing and Preservation · doi:10.1111/jfpp.14582 · Cited by 14
Feijoa [ Acca sellowiana (Berg) Burret] accessions characterization and discrimination by chemometrics
Sganzerla, W. G., Ferreira, A. L. A., Rosa, G. B., Azevedo, M. S., Ferrareze, J. P., Komatsu, R. A., Nunes, M. R., da Rosa, C. G., Schmit, R., Costa, M. D., et al. (2020) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.10585 · Cited by 14
Fitness of Outer Membrane Vesicles From Komagataeibacter intermedius Is Altered Under the Impact of Simulated Mars-like Stressors Outside the International Space Station
Podolich, O. V., Kukharenko, O. E., Zaets, I., Orlovska, I., Palchykovska, L. G., Zaika, L. A., Sysoliatin, S., Zubova, G., Reva, O. N., Galkin, M., et al. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.01268 · Cited by 14
“Lipid Analyses Total lipids were extracted from vesicle samples as recommended (Bligh and Dyer, 1959; Palmer, 1971).”
Method
The Droserasin 1 PSI: A Membrane-Interacting Antimicrobial Peptide from the Carnivorous Plant Drosera capensis
Sprague‐Piercy, M. A., Bierma, J. C., Crosby, M. G., et al. (2020) · Biomolecules · doi:10.3390/biom10071069 · Cited by 14
3 β -Hydroxysteroid- Δ 24 Reductase (DHCR24) Protects Pancreatic β Cells from Endoplasmic Reticulum Stress-Induced Apoptosis by Scavenging Excessive Intracellular Reactive Oxygen Species
Li, Y., Wang, X., Yang, B., Wang, H., Ma, Z., Lu, Z., Lu, X., & Gao, B. (2020) · Journal of Diabetes Research · doi:10.1155/2020/3426902 · Cited by 14
“An aliquot of the cells was used for cell counting, and the remaining cells were then subjected to the lipid extraction based on the method described by Bligh and Dyer [28].”
Method
Diacylglycerol kinase η regulates C2C12 myoblast proliferation through the mTOR signaling pathway
Sakai, H., Murakami, C., Usuki, T., Lü, Q., Matsumoto, K., Urano, T., & Sakane, F. (2020) · Biochimie · doi:10.1016/j.biochi.2020.07.018 · Cited by 16
Regiochemical Assignment of N ‑Acylphosphatidylethanolamines (NAPE) by Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry and Its Application to Extracts of Lupin Seeds
Bianco, M., Calvano, C. D., Ventura, G., et al. (2020) · Journal of the American Society for Mass Spectrometry · doi:10.1021/jasms.0c00267 · Cited by 14
Engineering an oleaginous yeast Candida tropicalis SY005 for enhanced lipid production
Chattopadhyay, A., Gupta, A., & Maiti, M. K. (2020) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-020-10830-6 · Cited by 14
Use of a Thermophile Desiccation-Tolerant Cyanobacterial Culture and Os Redox Polymer for the Preparation of Photocurrent Producing Anodes
Gacitúa, M. A., Urrejola, C., Carrasco, J., et al. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00900 · Cited by 14
“Total lipid extracts were obtained from samples according to a modified method of Bligh and Dyer (Bligh and Dyer, 1959).”
Method
Bacillus rugosus sp. nov. producer of a diketopiperazine antimicrobial, isolated from marine sponge Spongia officinalis L.
Bhattacharya, D., Santos‐Villalobos, S. D. L., Ruíz, V. V., et al. (2020) · Antonie van Leeuwenhoek · doi:10.1007/s10482-020-01472-9 · Cited by 14
“…was grown on TSB for polar lipid analysis, harvested at the exponential phase and the pellet was utilized for polar lipid extraction with methanol, chloroform and 0.3% sodium chloride (2:1:0.8 by volume) as described by Bligh and Dyer (1959) taking into account the amendments of Card (Card 1973).”
MethodInfluential
Fractional recovery of oleaginous bioactive produced by Rhodotorula mucilaginosa CCT3892 using deep eutectic solvents
da Costa, W. A., Padilha, C. E. D. A., Dantas, J. M. D. M., et al. (2020) · Bioresource Technology Reports · doi:10.1016/j.biteb.2020.100561 · Cited by 14
Variovorax beijingensis sp. nov., a novel plant-associated bacterial species with plant growth-promoting potential isolated from different geographic regions of Beijing, China
Gao, J.-L., Sun, Y., Xue, J., et al. (2020) · Systematic and Applied Microbiology · doi:10.1016/j.syapm.2020.126135 · Cited by 14
Early Pro-Inflammatory Remodeling of HDL Proteome in a Model of Diet-Induced Obesity: 2H2O-Metabolic Labeling-Based Kinetic Approach
Sadana, P., Lin, L., Aghayev, M., Ilchenko, S., & Kasumov, T. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21207472 · Cited by 14
“Liver tissue (100 mg) was homogenized in methanol and lipids were extracted in a chloroform:methanol (2:1, v/v) solvent mixture, as described previously [68].”
Method
Isolation of Microalgae from Mediterranean Seawater and Production of Lipids in the Cultivated Species
Haoujar, I., Cacciola, F., Manchado, M., Abrini, J., Haoujar, M., Chebbaki, K., Oteri, M., Rigano, F., Mangraviti, D., Mondello, L., et al. (2020) · Foods · doi:10.3390/foods9111601 · Cited by 16
“’s (2008) equation: PL = LT/(V culture × T) and % lipids = (LT/(CB×V culture))× 100; where PL was the lipid productivity (mg/L/day), LT was the total lipids (mg), T was the duration of the experiment, and V culture was the volume [36,37].”
Background
Pseudomonas lalkuanensis sp. nov., isolated from a bacterial consortia of contaminated soil enriched for the remediation of e-waste
Thorat, V., Kirdat, K., Tiwarekar, B., DaCosta, E., Debbarma, P., Shouche, Y. S., Sathe, S., Goel, R., Lodha, T. D., & Yadav, A. (2020) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.004559 · Cited by 14
MARGRA Lamb Eating Quality and Human Health-Promoting Omega-3 Long-Chain Polyunsaturated Fatty Acid Profiles of Tattykeel Australian White Sheep: Linebreeding and Gender Effects
Pewan, S. B., Otto, J. R., Kinobe, R. T., Adegboye, O. A., & Malau‐Aduli, A. E. O. (2020) · Antioxidants · doi:10.3390/antiox9111118 · Cited by 14
“Briefly, total lipids in 1 g of un-homogenised muscle tissue samples were extracted overnight using a modified Bligh and Dyer [46] method.”
Method
Corn Bioethanol Side Streams: A Potential Sustainable Source of Fat-Soluble Bioactive Molecules for High-Value Applications
Lena, G. D., del Pulgar, J. S., Lombardi‐Boccia, G., Casini, I., & Nicoli, S. F. (2020) · Foods · doi:10.3390/foods9121788 · Cited by 14
“Total lipids were extracted from the freeze-dried thin stillage (about 5 g) with methanol, chloroform, and water according to the method of Bligh and Dyer [12].”
Method
Polar Lipids Reduce In Vitro Duodenal Lipolysis Rate of Oat Oil and Liquid Oat Base Products
Yilmaz, J. L., Adlercreutz, P., & Tullberg, C. (2020) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.202000317 · Cited by 14
Fatty acids, health lipid indices, and cholesterol content of sheep meat of three breeds from Moroccan pastures
Belhaj, K., Mansouri, F., Benmoumen, A., Sindic, M., Fauconnier, M., Boukharta, M., Serghini‐Caid, H., & Elamrani, A. (2020) · Archives animal breeding/Archiv für Tierzucht · doi:10.5194/aab-63-471-2020 · Cited by 17
“IMF was extracted and quantified according to Bligh and Dyer (1959) using a mixture of chloroform / methanol / water mixture (2/1/1; v/v/v).”
Method
Improvement of Unsaturated Fatty Acid Production from Porphyridium cruentum Using a Two-Phase Culture System in a Photobioreactor with Light-Emitting Diodes (LEDs)
Kim, S., Lee, U. H., Lee, S. B., Jeong, G., & Kim, S.-K. (2020) · Journal of Microbiology and Biotechnology · doi:10.4014/jmb.2011.11004 · Cited by 16
“Total lipid content (% of DCW) was determined using methanol and chloroform following a modified solvent-based method [21] as shown in Eq.”
Method
Optimizing lime pretreatment of rice straw for biolipid production using oleaginous microorganisms
Doan, H. T., Phương, N. T. M., Tran, T. T., Nguyen, T. K., Tran, M. D., & Nguyen, D. B. (2020) · Chemosphere · doi:10.1016/j.chemosphere.2020.129390 · Cited by 14
Effects of Two Different Macroalgae (Ulva lactuca and Jania rubens) Species on Growth and Survival of Red Swamp Crayfish (Procambarus clarkii) as Feed Additive
Mazlum, Y., Yazıcı, M., Sayın, S., et al. (2020) · Marine Science and Technology Bulletin · doi:10.33714/masteb.820627 · Cited by 14
“The ash, lipid and protein amounts of macroalgae were determined by following the methods of Vollenweider (1974) and Bligh & Dyer (1959).”
Method
Omega 3 fatty acids stimulate thermogenesis during torpor in the Arctic Ground Squirrel
Rice, S. A., Mikes, M. L., Bibus, D. M., Berdyshev, E. V., Reisz, J. A., Gehrke, S. A., Bronova, I. A., D’Alessandro, A., & Drew, K. L. (2021) · Scientific Reports · doi:10.1038/s41598-020-78763-8 · Cited by 15
Nutritional composition and bioactive compounds of Melipona seminigra pot‐pollen from Amazonas, Brazil
Rebelo, K. S., Cazarin, C. B. B., Iglesias, A. H., Stahl, M. A., Kristiansen, K., Carvalho-Zilse, G. A., Grimaldi, R., Reyes, F. G. R., Danneskiold‐Samsøe, N. B., & Maróstica, M. R. (2021) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.11134 · Cited by 17
Identification of the Flavobacterium johnsoniae cysteate‐fatty acyl transferase required for capnine synthesis and for efficient gliding motility
Vences‐Guzmán, M. Á., Peña‐Miller, R., Hidalgo‐Aguilar, N. A., Vences‐Guzmán, M. L., Guan, Z., & Sohlenkamp, C. (2021) · Environmental Microbiology · doi:10.1111/1462-2920.15445 · Cited by 15
NF-κB pathway play a role in SCD1 deficiency-induced ceramide de novo synthesis
Zheng, R., Zhang, Y., Qiu, F., Liu, Z., Han, Y., Huang, R., Zhao, Y., Yao, P., Qiu, Y., & Ren, J. (2021) · Cancer Biology & Therapy · doi:10.1080/15384047.2021.1883414 · Cited by 14
“Lipids were extracted from cells as described previously.(28) Briefly, 10(6) cells were ultrasonicated in a methanol/water mixture (vol/vol, 1/1, 1 ml) containing two internal standards: C17:0 ceramide (100 pmol, internal standard for ceramides) and C17:0 margaric acid (100 pmol, internal standard for fatty acids).”
Method
Temperature induced changes in the development of embryos and paralarvae of the merobenthic octopus, Octopus tetricus
Spreitzenbarth, S., & JEFFS, A. G. (2021) · Aquaculture · doi:10.1016/j.aquaculture.2021.736547 · Cited by 16
β-Sitosterol 3-O-D-glucoside increases ceramide levels in the stratum corneum via the up-regulated expression of ceramide synthase-3 and glucosylceramide synthase in a reconstructed human epidermal keratinization model
Takeda, S., Terazawa, S., Shimoda, H., & Imokawa, G. (2021) · PLoS ONE · doi:10.1371/journal.pone.0248150 · Cited by 15
“Bligh-Dyer method described in previous studies [17, 38] was used for lipid extraction.”
Method
Rhodotorula Strains Isolated from Seawater That Can Biotransform Raw Glycerol into Docosahexaenoic Acid (DHA) and Carotenoids for Animal Nutrition
Pino-Maureira, N. L., González-Saldía, R. R., Capdeville, A., et al. (2021) · Applied Sciences · doi:10.3390/app11062824 · Cited by 14
“5-N KOH: MeOH [20], and the non-saponifi able lipids were separated with hexane.”
MethodInfluential
Investigation of phenolic compounds and antioxidant activity in red and yellow onions and a synergistic utilization of skin extract in modified atmosphere packaging of salmon ( Salmo salar )
GÜNER, S., Boz, Z., Yağız, Y., Topalcengiz, Z., Welt, B. A., Sarnoski, P. J., Simonne, A., Kristinsson, H. G., & Marshall, M. R. (2021) · Packaging Technology and Science · doi:10.1002/pts.2568 · Cited by 15
“For this purpose, lipid extraction was conducted by following the method of Bligh and Dyer.(34) For FAME analysis, the method of Yagiz et al.”
MethodInfluential
Cannabinoid type 1 receptor inverse agonism attenuates dyslipidemia and atherosclerosis in APOE∗3-Leiden.CETP mice
van Eenige, R., Ying, Z., Tambyrajah, L. L., Pronk, A. C. M., Blomberg, N., Giera, M. A., Wang, Y., Coşkun, T., van der Stelt, M., Rensen, P. C. N., et al. (2021) · Journal of Lipid Research · doi:10.1016/j.jlr.2021.100070 · Cited by 15
“Liver lipids were extracted according to a modified protocol of Bligh and Dyer (29).”
Method
Very low-density lipoprotein receptor increases in a liver-specific manner due to protein deficiency but does not affect fatty liver in mice
Oshio, Y., Hattori, Y., Kamata, H., Ozaki‐Masuzawa, Y., Seki, A., Tsuruta, Y., & Takenaka, A. (2021) · Scientific Reports · doi:10.1038/s41598-021-87568-2 · Cited by 13
Development of Functional Acid Curd Cheese (Tvarog) with Antioxidant Activity Containing Astaxanthin from Shrimp Shells Preliminary Experiment
Dmytrów, I., Szymczak, M., Szkolnicka, K., & Kamiński, P. (2021) · Foods · doi:10.3390/foods10040895 · Cited by 14
“Lipids from ACC samples were extracted using the method of Bligh and Dyer [26].”
Method
Proteomics and expression studies on lipids and fatty acids metabolic genes in Isochrysis galbana under the combined influence of nitrogen starvation and sodium acetate supplementation
Kaur, S., Hérault, J., Caruso, A., Pencreach, G., Côme, M., Gauvry, L., Claverol, S., & Loiseau, C. (2021) · Bioresource Technology Reports · doi:10.1016/j.biteb.2021.100714 · Cited by 15
Nuclear Sphingosine-1-phosphate Lyase Generated ∆2-hexadecenal is A Regulator of HDAC Activity and Chromatin Remodeling in Lung Epithelial Cells
Ebenezer, D. L., Ramchandran, R., Fu, P., Mangio, L. A., Suryadevara, V., Ha, A. W., Berdyshev, E. V., Veldhoven, P. P. V., Kron, S. J., Schumacher, F., et al. (2021) · Cell Biochemistry and Biophysics · doi:10.1007/s12013-021-01005-9 · Cited by 15
Effect of Breed and Diet on Carcass Parameters and Meat Quality of Spent Hens
Vargas‐Ramella, M., Pateiro, M., Rois, D., et al. (2021) · Annals of Animal Science · doi:10.2478/aoas-2021-0036 · Cited by 14
Identifying metabolic alterations associated with coral growth anomalies using 1H NMR metabolomics
Andersson, E. R., Day, R. D., Work, T. M., et al. (2021) · Coral Reefs · doi:10.1007/s00338-021-02125-7 · Cited by 14
“Metabolites were extracted from the tissue powders (all samples were standardized to 100 mg ± 3 mg prior to extraction) using methods modified from Bligh and Dyer (1959) and Wu et al. (2008), as recommended for reefbuilding corals by Andersson et al. (2019).”
Method
N -(4-Hydroxyphenyl) Retinamide Suppresses SARS-CoV-2 Spike Protein-Mediated Cell-Cell Fusion by a Dihydroceramide Δ4-Desaturase 1-Independent Mechanism
Hayashi, Y., Tsuchiya, K., Yamamoto, M., et al. (2021) · Journal of Virology · doi:10.1128/jvi.00807-21 · Cited by 14
Docosahexaenoic Acid Is Naturally Concentrated at the sn-2 Position in Triacylglycerols of the Australian Thraustochytrid Aurantiochytrium sp. Strain TC 20
Chang, K. J. L., Taylor, M. C., Drummond, G., Mulder, R. J., Mansour, M. P., Brock, M., & Nichols, P. D. (2021) · Marine Drugs · doi:10.3390/md19070382 · Cited by 14
“Samples (100 mg of freeze dried biomass) were extracted quantitatively by the modified dichloromethane (DCM)-methanol (MeOH)-water Bligh and Dyer (1959) method [25].”
Method
Phytoplankton dynamics and renewable energy potential induced by the environmental conditions of Lake Burullus, Egypt
Masoud, A. A., El-Horiny, M. M., Khairy, H. M., & El-Sheekh, M. M. (2021) · Environmental Science and Pollution Research · doi:10.1007/s11356-021-15625-4 · Cited by 13
“According to Bligh and Dyer (1959), total lipid content was measured gravimetrically after extraction in chloroform/methanol (2:1 v/v).”
Method
Increased endothelial sodium channel activity by extracellular vesicles in human aortic endothelial cells: putative role of MLP1 and bioactive lipids
Nouri, M., Yu, L., Liu, L., Chacko, K. M., Denslow, N. D., LaDisa, J. F., & Alli, A. A. (2021) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00092.2020 · Cited by 13
Serine/Glycine Lipid Recovery in Lipid Extracts From Healthy and Diseased Dental Samples: Relationship to Chronic Periodontitis
Nichols, F. C., Bhuse, K., Clark, R. B., et al. (2021) · Frontiers in Oral Health · doi:10.3389/froh.2021.698481 · Cited by 14
“Lipid Extraction Procedure Lipids were extracted from bacterial pellets, teeth, subgingival plaque, and homogenized gingival tissue samples using the phospholipid extraction procedure of Bligh and Dyer [11] as modified by Garbus [12].”
MethodInfluential
Late endosomal/lysosomal accumulation of a neurotransmitter receptor in a cellular model of Smith‐Lemli‐Opitz syndrome
Sharma, A., Kumar, G. A., & Chattopadhyay, A. (2021) · Traffic · doi:10.1111/tra.12811 · Cited by 14
“Lipids from cellular lysates were extracted according to the Bligh and Dyer method.(79)”
Method
New approach in determination of urinary diagnostic markers for prostate cancer by MALDI-TOF/MS
Buszewska‐Forajta, M., Pomastowski, P. P., Monedeiro, F., et al. (2021) · Talanta · doi:10.1016/j.talanta.2021.122843 · Cited by 14
Engineering the Outer Membrane Could Facilitate Better Bacterial Performance and Effectively Enhance Poly-3-Hydroxybutyrate Accumulation
Wang, J., Ma, W., Fang, Y., et al. (2021) · Applied and Environmental Microbiology · doi:10.1128/aem.01389-21 · Cited by 14
“0) using the modified Bligh-Dyer method according to our previous study (30, 53).”
Method
Biocatalytic transesterification of algal oil employing a heterogenous methanol tolerant lipase enzyme aggregate from Bacillus mycoides strain CV18
Sharma, D., Uniyal, S., & Tewari, L. (2021) · Process Biochemistry · doi:10.1016/j.procbio.2021.10.005 · Cited by 14
Production and economics of probiotics treated Macrobrachium rosenbergii at different stocking densities
Azad, M. A. K., Islam, S. S., Amin, M. N., et al. (2021) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2021.115125 · Cited by 14
Chemical characteristics and volatile compounds profiles in different muscle part of the farmed hybrid catfish ( Clarias macrocephalus × Clarias gariepinus )
Phetsang, H., Panpipat, W., Panya, A., et al. (2021) · International Journal of Food Science & Technology · doi:10.1111/ijfs.15419 · Cited by 14
Mitochondrial Phospholipid Homeostasis Is Regulated by the i-AAA Protease PaIAP and Affects Organismic Aging
Löser, T., Joppe, A., Hamann, A., & Osiewacz, H. D. (2021) · Cells · doi:10.3390/cells10102775 · Cited by 15
“Phospholipid (PL) extraction from isolated mitochondria was performed as described by Bligh and Dyer [37].”
Method
Determination of fatty acids and vitamins A, D and E intake through fish consumption
Yerlikaya, P., Can, A. B., Tokay, F. G., et al. (2021) · International Journal of Food Science & Technology · doi:10.1111/ijfs.15435 · Cited by 14

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks