Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 30,501–30,600 of 48,846

Effects of Long-Chain Polyunsaturated Fatty Acids in Combination with Lutein and Zeaxanthin on Episodic Memory in Healthy Older Adults
Sueyasu, T., Yasumoto, K., Tokuda, H., Kaneda, Y., Obata, H., Rogi, T., Izumo, T., Kondo, S., Saito, J., Tsukiura, T., et al. (2023) · Nutrients · doi:10.3390/nu15132825 · Cited by 16
“Lipids were extracted and purified from the plasma according to the method of the previous report [33], and then thin-layer chromatography (hexane:ether = 7:3) was used to separate the phospholipid fraction.”
Method
Quality and composition of meat in different productive types of domestic quail
Lukanov, H., Pavlova, I., Genchev, A., et al. (2023) · Journal of Central European Agriculture · doi:10.5513/jcea01/24.2.3871 · Cited by 14
Effect of protein addition in properties of gels produced with jackfruit (Artocarpus integrifolia) seed starch: rheological and texture properties
Barbosa, M. C., Silva, G. L., Viana, E. B. M., et al. (2023) · Journal of Food Science and Technology · doi:10.1007/s13197-023-05793-1 · Cited by 14
Mapping the microbial diversity associated with different geochemical regimes in the shallow-water hydrothermal vents of the Aeolian archipelago, Italy
Barosa, B., Ferrillo, A., Selci, M., et al. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1134114 · Cited by 14
Extension of shelf life of Nile tilapia (Oreochromis niloticus) fillets using seaweed extracts during refrigerated storage
Shahrier, J., Rasul, M. G., Afrin, F., et al. (2023) · Food Science & Nutrition · doi:10.1002/fsn3.3673 · Cited by 14
“The total lipid of fish fillets was extracted following the method described by Bligh and Dyer (1959).”
Method
Pea Protein Isolate as a Meat Substitute in Canned Pork Pâté: Nutritional, Technological, Oxidative, and Sensory Properties
Trindade, P. C. O., dos Santos, B. A., Hollweg, G., et al. (2023) · Foods · doi:10.3390/foods12183486 · Cited by 14
“Lipids were determined using the Bligh and Dyer [8] method.”
Choline metabolism underpins macrophage IL-4 polarization and RELMα up-regulation in helminth infection
Ghorbani, P., Kim, S. Y., Smith, T. K. T., Minarrieta, L., Robert-Gostlin, V. N., Kilgour, M. K., Ilijevska, M., Alecu, I., Snider, S. A., Margison, K. D., et al. (2023) · PLoS Pathogens · doi:10.1371/journal.ppat.1011658 · Cited by 15
Cholesterol-dependent homeostatic regulation of very long chain sphingolipid synthesis
Kim, Y., Mavodza, G. R., Senkal, C. E., & Burd, C. G. (2023) · The Journal of Cell Biology · doi:10.1083/jcb.202308055 · Cited by 15
“…labeling experiments, total cell and medium (M β CD bound) lipids were extracted with solvents containing chloroform (9180-01; JT Baker), methanol (9070-05; JT Baker), and water (purified by Millipore Water System) by 1:1:0.9 (vol/ vol) based on a modified Bligh-Dyer method (Bligh and Dyer, 1959).”
MethodInfluential
Development of sustainable downstream processing for nutritional oil production
Rollin, S., Gupta, A., Franco, C. M. M., Singh, S. P., & Puri, M. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1227889 · Cited by 18
Cold‐inducible lncRNA266 promotes browning and the thermogenic program in white adipose tissue
Ma, J., Wu, Y., Cen, L., Wang, Z., Jiang, K., Lian, B., & Sun, C. (2023) · EMBO Reports · doi:10.15252/embr.202255467 · Cited by 13
“Total lipid extraction from the liver was performed with a previous method (Bligh & Dyer, 1959).”
Method
Human Cerebrospinal Fluid Sample Preparation and Annotation for Integrated Lipidomics and Metabolomics Profiling Studies
Hooshmand, K., Xu, J., Simonsen, A. H., Wretlind, A., de Zawadzki, A., Sulek, K., Hasselbalch, S. G., & Legido‐Quigley, C. (2023) · Molecular Neurobiology · doi:10.1007/s12035-023-03666-4 · Cited by 15
Impact of frozen storage on quality and cold storage stability of smoked deep-skinned fillets from well-fed Atlantic mackerel
Fernandes, C. M., Sveinsdóttir, H. I., Tómasson, T., et al. (2023) · Journal of Food Composition and Analysis · doi:10.1016/j.jfca.2023.105771 · Cited by 14
Effect of Farming System and Season on Proximate Composition, Fatty Acid Profile, Antioxidant Activity, and Physicochemical Properties of Retail Cow Milk
Kasapidou, E., Stergioudi, R.-A., Papadopoulos, V., Mitlianga, P. G., Papatzimos, G., Karatzia, M.-A., Amanatidis, M., Tortoka, V., Tsiftsi, E., Aggou, A., et al. (2023) · Animals · doi:10.3390/ani13233637 · Cited by 14
“Milk samples were thawed overnight at 4 ◦ C, and the following day the milk lipids were extracted with a chloroform/methanol solution (1:2 v / v ) according to the method of Bligh and Dyer [16].”
Method
Late Pleistocene to modern precipitation changes at the Paranal clay pan, central Atacama Desert
Wennrich, V., Böhm, C., Brill, D., Carballeira, R., Hoffmeister, D., Jaeschke, A., Kerber, F., Maldonado, A., May, S. M., Olivares, L., et al. (2023) · Global and Planetary Change · doi:10.1016/j.gloplacha.2023.104349 · Cited by 16
Alveolar macrophage lipid burden correlates with clinical improvement in patients with pulmonary alveolar proteinosis
Lee, E., Williams, K. J., McCarthy, C., Bridges, J. P., Redente, E. F., Vallim, T. Q. D. A., Barrington, R. A., Wang, T. S., & Tarling, E. J. (2024) · Journal of Lipid Research · doi:10.1016/j.jlr.2024.100496 · Cited by 14
“A modified Bligh and Dyer extraction was performed on human alveolar macrophages (21, 22).”
Method
Integrative Metabolomics and Proteomics Allow the Global Intracellular Characterization of Bacillus subtilis Cells and Spores
Huang, Y., Swarge, B. N., Roseboom, W., Bleeker, J., Brul, S., Setlow, P., & Kramer, G. (2024) · Journal of Proteome Research · doi:10.1021/acs.jproteome.3c00386 · Cited by 14
NF1 deficiency drives metabolic reprogramming in ER+ breast cancer
House, R. J., Tovar, E. A., Redlon, L. N., Essenburg, C. J., Dischinger, P. S., Ellis, A. E., Beddows, I., Sheldon, R. D., Lien, E. C., Graveel, C. R., et al. (2024) · Molecular Metabolism · doi:10.1016/j.molmet.2024.101876 · Cited by 15
Challenging old microbiological treasures for natural compound biosynthesis capacity
Nouioui, I., Zimmermann, A., Hennrich, O., Xia, S.-N., Rössler, O., Makitrynskyy, R., Gomez‐Escribano, J. P., Pötter, G., Jando, M., Döppner, M., et al. (2024) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2024.1255151 · Cited by 16
Comparing the Extraction Performance in Mouse Plasma of Different Biphasic Methods for Polar and Nonpolar Compounds
Gutmann, F., Fritsche‐Guenther, R., Dias, D. B., et al. (2024) · Journal of Proteome Research · doi:10.1021/acs.jproteome.3c00596 · Cited by 14
“We compared a modified SCIEX Lipidyzer extraction protocol employing MTBE, a modified MTBE method by Matyash et al. (2008), and an adapted protocol of the well-established Bligh − Dyer (1959; BD) method to assess their overall combined performance for polar and nonpolar targeted metabolite analysis.”
BackgroundMethod
Pork belly quality variation and its association with fatness level
Albano‐Gaglio, M., Zomeño, C., Tejeda, J. F., et al. (2024) · Meat Science · doi:10.1016/j.meatsci.2024.109482 · Cited by 14
High throughput screening of mesenchymal stromal cell morphological response to inflammatory signals for bioreactor-based manufacturing of extracellular vesicles that modulate microglia
Larey, A. M., Spoerer, T. M., Daga, K. R., Morfin, M. G., Hynds, H. M., Carpenter, J. M., Hines, K. M., & Marklein, R. A. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2024.03.009 · Cited by 13
Metabolomic and Lipidomic Tools for Tracing Fish Escapes from Aquaculture Facilities
Badaoui, W., Marhuenda-Egea, F. C., Valero-Rodriguez, J. M., Sánchez‐Jerez, P., Arechavala‐Lopez, P., & Toledo‐Guedes, K. (2024) · ACS Food Science & Technology · doi:10.1021/acsfoodscitech.3c00589 · Cited by 14
Staphylococcus aureus oleate hydratase produces ligands that activate host PPARα
Radka, C. D., Frank, M. W., Simmons, T. S., Johnson, C. N., Rosch, J. W., & Rock, C. O. (2024) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2024.1352810 · Cited by 14
Microwave-Assisted Extraction/UHPLC-Q-Orbitrap-MS-Based Lipidomic Workflow for Comprehensive Study of Lipids in Soft Cheese
Campaniello, M., Nardelli, V., Zianni, R., Tomaiuolo, M., Miedico, O., Iammarino, M., & Mentana, A. (2024) · Foods · doi:10.3390/foods13071033 · Cited by 15
“Considering that lipid isolation follows the laws of solid–liquid extraction, it is common to perform MAE using the same solvent prescribed for traditional extraction, such as Folch [6] or Bligh and Dyer [7].”
BackgroundMethod
The Diversity and Composition of Soil Microbial Communities Differ in Three Land Use Types of the Sanjiang Plain, Northeastern China
Wang, S., Wang, M., Gao, X. D., et al. (2024) · Microorganisms · doi:10.3390/microorganisms12040780 · Cited by 14
“The individual characteristic PLFA biomarker amount was obtained by converting the reaction value of the internal standard 18:00 in ng/g with the following equation:”
Method
Defective pgsA contributes to increased membrane fluidity and cell wall thickening in Staphylococcus aureus with high-level daptomycin resistance
Freeman, C. D., Hansen, T., Urbauer, R. J. B., Wilkinson, B. J., Singh, V. K., & Hines, K. M. (2024) · mSphere · doi:10.1128/msphere.00115-24 · Cited by 14
Infant Formula with 50% or More of Palmitic Acid Bound to the sn-2 Position of Triacylglycerols Eliminate the Association between Formula-Feeding and the Increase of Fecal Palmitic Acid Levels in Newborns: An Exploratory Study
Shoji, H., Arai, H., Kakiuchi, S., Ito, A., Sato, K., Jinno, S., Takahashi, N., Masumoto, K., Yoda, H., & Shimizu, T. (2024) · Nutrients · doi:10.3390/nu16111558 · Cited by 14
Supplementation of Seaweed Extracts to the Diet Reduces Symptoms of Alzheimer’s Disease in the APPswePS1ΔE9 Mouse Model
Martens, N., Zhan, N., Yam, S. C., Leijten, F. P. J., Palumbo, M., Caspers, M. P. M., Tiane, A., Friedrichs, S., Li, Y., der Zee, L. V. V., et al. (2024) · Nutrients · doi:10.3390/nu16111614 · Cited by 16
“Lipid extraction from liver homogenates was performed as described by Bligh and Dyer [32].”
Method
Fatty Acid Profile and Thermal Behavior of Fat-Rich Edible Insect Oils Compared to Commonly Consumed Animal and Plant Oils
Chantakun, K., Petcharat, T., Wattanachant, S., Karim, S. A., & Kaewthong, P. (2024) · Food Science of Animal Resources · doi:10.5851/kosfa.2024.e44 · Cited by 16
“Oils extracted from all the samples (Bligh and Dyer, 1959) were examined for their fatty acid profiles using gas chromatography (GC) and thermal behaviors (melting and crystallization profiles) using differential scanning calorimetry (DSC).”
Method
Evaluation of Extraction Techniques for Recovery of Microalgal Lipids under Different Growth Conditions
Bhowmick, G. D., Plouviez, M., Reis, M. G., Guieysse, B., Everett, D. W., Agnew, M. P., Maclean, P. H., & Thum, C. (2024) · ACS Omega · doi:10.1021/acsomega.4c00221 · Cited by 15
Rancidity development in oat during industrial processing
Pålsson, D., Penttinen, H., Malmberg, C., et al. (2024) · LWT · doi:10.1016/j.lwt.2024.116448 · Cited by 14
Probiotic therapy modulates the brain-gut-liver microbiota axis in a mouse model of traumatic brain injury
Amaral, W. Z., Kokroko, N., Treangen, T. J., Villapol, S., & Gómez‐Pinilla, F. (2024) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2024.167483 · Cited by 15
Detailed lipid investigation of edible seaweeds by photochemical derivatization and untargeted lipidomics
Montone, C. M., Cavaliere, C., Cerrato, A., Laganà, A., Piovesana, S., Taglioni, E., & Capriotti, A. L. (2024) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-024-05573-6 · Cited by 14
Simultaneous Determination of the Position and Cis–Trans Configuration of Lipid C═C Bonds via Asymmetric Derivatization and Ion Mobility–Mass Spectrometry
Tang, S., Wang, H., Zhang, H., et al. (2024) · Journal of the American Chemical Society · doi:10.1021/jacs.4c08980 · Cited by 14
Cover crop systems impact on biomass production, carbon-to-nitrogen ratio, forage quality, and soil health in a semi-arid environment
Opoku, A., Ogunleye, A. M., Solomon, J. K. Q., & Payne, W. A. (2024) · Heliyon · doi:10.1016/j.heliyon.2024.e39600 · Cited by 15
“The microbial community structure and biomass of soil from the different cover crop treatments were determined by phospholipid fatty acid analysis (PLFA) for total lipid extraction based on a modified version of the method of Bligh and Dyer [45] by Buyer et al. [46].”
Method
Structure and mechanism of biosynthesis of Streptococcus mutans cell wall polysaccharide
Rush, J. S., Zamakhaeva, S. A., Murner, N. R., Deng, P., Morris, A. J., Kenner, C. W., Black, I. M., Heiß, C., Azadi, P., Korotkov, K. V., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-56205-1 · Cited by 16
Regulation of mitochondrial cristae organization by Myo19, Miro1 and Miro2, and metaxin 3
Shembekar, S., Nikolaus, P., Honnert, U., Höring, M., Attia, A., Topp, K., Lohmann, B., Liebisch, G., & Bähler, M. (2025) · Journal of Cell Science · doi:10.1242/jcs.263637 · Cited by 14
Impact of ultrasound-assisted intermittent hydration during pumpkin seed germination on the structure, nutritional, bioactive, physical and techno-functional properties of flours
Pacheco, F. C., Andressa, I., Pacheco, A. F. C., et al. (2025) · LWT · doi:10.1016/j.lwt.2025.117654 · Cited by 14
Measurement of Mammalian Diacylglycerol Kinase Activity In Vitro and in Cells
Epand, R. M., & Topham, M. K. (2007) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(07)34016-0 · Cited by 14
Inverse association between docosahexaenoic acid and mortality in patients on hemodialysis during over 10 years
Terashima, Y., Hamazaki, K., Itomura, M., Tomita, S., Kuroda, M., Hirata, H., Hamazaki, T., & Inadera, H. (2014) · Hemodialysis International · doi:10.1111/hdi.12128 · Cited by 14
Modest Decrease in Pgc1α Results in TAG Accumulation but not in Insulin Resistance in L6 Myotubes
Łukaszuk, B., Mikłosz, A., Chabowski, A., & Górski, J. (2015) · Cellular Physiology and Biochemistry · doi:10.1159/000373975 · Cited by 14
Phospholipid Fatty Acid Composition of the Syntrophic Anaerobic Bacterium Syntrophomonas wolfei
Henson, J. M., McInerney, M. J., Beaty, P. S., et al. (1988) · Applied and Environmental Microbiology · doi:10.1128/aem.54.6.1570-1574.1988 · Cited by 14
Growth, total lipid content and fatty acid profile of a native strain of the freshwater oleaginous microalgae Ankistrodesmus falcatus (Ralf) grown under salt stress condition.
Talukdar, J., Chandra, K., & Chandra, G. (2012) · Cited by 13
A chronsequential approach to investigating microbial community shifts following clearcutting in Boreal Plain forest soils
Hynes, H. M., & Germida, J. J. (2012) · Canadian Journal of Forest Research · doi:10.1139/cjfr-2012-0038 · Cited by 14
Enzyme activity, microbial biomass and community structure in a long-term restored soil under semi-arid conditions
Torres, I. F., Bastida, F., Hernández, T., et al. (2015) · Soil Research · doi:10.1071/sr14297 · Cited by 14
Skin lipid profiling in normal and seborrhoeic shih tzu dogs
Yoon, J., Nishifuji, K., Ishioroshi, S., Ide, K., & Iwasaki, T. (2013) · Veterinary Dermatology · doi:10.1111/j.1365-3164.2012.01102.x · Cited by 14
Efecto del tipo de músculo sobre parámetros de calidad en carne fresca de cerdo ibérico
Muriel, E., Antequera, T., & Ruiz, J. (2002) · Ciencia y Tecnologia Alimentaria · Cited by 14
Lipid signatures of acidophilic microbial communities in an extreme acidic environment – Río Tinto, Spain
Bühring, S. I., Schubotz, F., Harms, C., et al. (2012) · Organic Geochemistry · doi:10.1016/j.orggeochem.2012.03.010 · Cited by 14
Growth performance, carcass traits, meat chemical composition and blood serum metabolites of broiler chicken fed on diets containing flaxseed oil
Lopes, D. C., Xavier, E. G., Santos, V. L., et al. (2013) · British Poultry Science · doi:10.1080/00071668.2013.843161 · Cited by 14
Two strings to the systems biology bow: co-extracting the metabolome and proteome of yeast
Schmidt, S. A., Jacob, S. S., Ahn, S. B., Rupasinghe, T., Krömer, J. O., Khan, A., & Varela, C. (2012) · Metabolomics · doi:10.1007/s11306-012-0437-1 · Cited by 14
Obestatin and Nt8U influence glycerolipid metabolism and PPAR gamma signaling in mice
Nagaraj, S., Raghavan, A. V., Rao, S. N., & Manjappara, U. V. (2014) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2014.06.006 · Cited by 14
Detection and characterization of phosphatidylcholine in various strains of the genus Chlamydomonas (Volvocales, Chlorophyceae)
Sakurai, K., Mori, N., & Sato, N. (2014) · Journal of Plant Research · doi:10.1007/s10265-014-0644-0 · Cited by 14
Effects Of Ficus Thonningii Extracts On The Gastrointestinal Tract And Clinical Biochemistry Of Suckling Rats
Dangarembizi, R., Erlwanger, K. H., & Chivandi, E. (2014) · African Journal of Traditional Complementary and Alternative Medicines · doi:10.4314/ajtcam.v11i2.10 · Cited by 14
Influence of terrestrial lipid and protein sources and activated carbon-treated fish oil on levels of persistent organic pollutants and fatty acids in the flesh of Atlantic salmon
Friesen, E. N., Skura, B. J., Ikonomou, M. G., et al. (2013) · Aquaculture Research · doi:10.1111/are.12182 · Cited by 14
Seasonal changes in morphometric and biochemical endpoints in northern pike (Esox lucius), burbot (Lota lota) and slimy sculpin (Cottus cognatus)
Bennett, P. M., & Janz, D. M. (2007) · Freshwater Biology · doi:10.1111/j.1365-2427.2007.01819.x · Cited by 14
An intervention study demonstrates effects of genotype on boar taint and performances of growing–finishing pigs
den Broeke, A. V., Aluwé, M., Tuyttens, F., et al. (2015) · Journal of Animal Science · doi:10.2527/jas.2014-8184 · Cited by 14
The Anatomical Origin of Raw Meat Affects the Sensory and Chemical Characteristics of a Dry-Cured Beef Product: Cecina de León
Molinero, C., Martínez, B., Rubio, B., et al. (2008) · Food Science and Technology International · doi:10.1177/1082013208095690 · Cited by 14
Chemoenzymatic Conversion of Linoleic Acid into Conjugated Linoleic Acid
DEMİR, A. S., & Talpur, F. N. (2010) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf903521b · Cited by 14
Utilization of Shochu Distillery By-products for Culturing the Common Carp Cyprinus carpio L.
., J. F. M., Yamasaki, S., & Onoue, Y. (2003) · Journal of Biological Sciences · doi:10.3923/jbs.2003.502.507 · Cited by 14
The responses of rainbow trout gills to high lithium and potassium concentrations in water
Tkatcheva, V., Holopainen, I. J., Hyvärinen, H., et al. (2007) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2007.03.008 · Cited by 14
Endothelial progenitor cells express PAF receptor and respond to PAF via Ca2+-dependent signaling
Balestrieri, M. L., Giovane, A., Milone, L., & Servillo, L. (2010) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2010.07.001 · Cited by 14
Revisiting the expression and purification of MGD1, the major galactolipid synthase in Arabidopsis to establish a novel standard for biochemical and structural studies
Rocha, J., Audry, M., Pesce, G., et al. (2012) · Biochimie · doi:10.1016/j.biochi.2012.11.011 · Cited by 14
Echium oil is better than rapeseed oil in improving the response of barramundi to a disease challenge
Alhazzaa, R., Bridle, A. R., Mori, T. A., Barden, A. E., Nichols, P. D., & Carter, C. G. (2013) · Food Chemistry · doi:10.1016/j.foodchem.2013.04.052 · Cited by 14
Inhibition of Endothelial Lipase Activity by Sphingomyelin in the Lipoproteins
Yang, P., Belikova, N. A., Billheimer, J. T., et al. (2014) · Lipids · doi:10.1007/s11745-014-3944-1 · Cited by 14
Rapid determination of biosynthetic pathways using fractional isotope enrichment and high‐resolution dynamic nuclear polarization enhanced NMR
Bowen, S., Sekar, G., & Hilty, C. (2011) · NMR in Biomedicine · doi:10.1002/nbm.1679 · Cited by 14
Produção de salgadinho extrusado de quirera de arroz para uso na indústria de alimentos
Limberger, V. M., Comarela, C. G., Patias, L. D., et al. (2009) · Ciência Rural · doi:10.1590/s0103-84782009000900032 · Cited by 14
In vivo response of melatonin, gonadal activity and biochemical changes during CYP19 inhibited sex reversal in common carp Cyprinus carpio (L)
Singh, A. K., & Singh, R. (2012) · Animal Reproduction Science · doi:10.1016/j.anireprosci.2012.11.004 · Cited by 14
Effects of high‐pressure homogenisation on physicochemical characteristics of partially skimmed milk
Pedras, M. M., Tribst, A. A. L., & Cristianini, N. (2013) · International Journal of Food Science & Technology · doi:10.1111/ijfs.12378 · Cited by 14
Gas Chromatographic Separation of Docosenoic Acid Positional Isomers on an SLB-IL100 Ionic Liquid Column
Shimizu, K., & Ando, Y. (2012) · Journal of Oleo Science · doi:10.5650/jos.61.421 · Cited by 14
Effects of inhibitors of key enzymes of sphingolipid metabolism on insulin-induced glucose uptake and glycogen synthesis in liver cells of old rats
Babenko, N. A., & Kharchenko, V. S. (2015) · Biochemistry (Moscow) · doi:10.1134/s0006297915010125 · Cited by 15
Acinar inflammatory response to lipid derivatives generated in necrotic fat during acute pancreatitis
Mateu, A., Ramudo, L., Manso, M. A., Closa, D., & DIOS, I. D. (2014) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2014.06.016 · Cited by 14
Cholesteryl Esters Are Elevated in the Lipid Fraction of Bronchoalveolar Lavage Fluid Collected from Pediatric Cystic Fibrosis Patients
Ma, D. C., Yoon, A. J., Faull, K. F., et al. (2015) · PLoS ONE · doi:10.1371/journal.pone.0125326 · Cited by 14
Glycosphingolipid storage leads to the enhanced degradation of the B cell receptor in Sandhoff disease mice
Vruchte, D. T., Jeans, A., Platt, F. M., & Sillence, D. J. (2010) · Journal of Inherited Metabolic Disease · doi:10.1007/s10545-010-9109-3 · Cited by 14
Anionic Lipid Binding to the Foreign Protein MGS Provides a Tight Coupling between Phospholipid Synthesis and Protein Overexpression in Escherichia coli
Ariöz, C., Ye, W., Bakali, A., et al. (2013) · Biochemistry · doi:10.1021/bi400616n · Cited by 14
Identification and quantification of triacylglycerols containing n-3 long-chain polyunsaturated fatty acids in bovine milk
Liu, Z., Moate, P. J., Ezerniks, V., Cocks, B. G., & Rochfort, S. J. (2015) · Journal of Dairy Science · doi:10.3168/jds.2015-9889 · Cited by 14
Cobalt carbonyl complexes as probes for alkyne-tagged lipids
Tallman, K. A., Armstrong, M. D., Milne, S. B., et al. (2013) · Journal of Lipid Research · doi:10.1194/jlr.d033332 · Cited by 14
Lipid body formation by Thraustochytrium aureum (ATCC 34304) in response to cell age
Jeh, E.-J., Kumaran, R. S., & Hur, B. (2008) · Korean Journal of Chemical Engineering · doi:10.1007/s11814-008-0180-5 · Cited by 14
EFEITO DAS CONDIÇÕES DE EXTRAÇÃO NO RENDIMENTO E QUALIDADE DO LEITE DE CASTANHA-DO-BRASIL DESPELICULADA
FERBERG, I., Cabral, L. C., Gonçalves, E. B., et al. (2002) · Boletim do Centro de Pesquisa de Processamento de Alimentos · doi:10.5380/cep.v20i1.1137 · Cited by 14
Low levels of dietary vitamin A increase intramuscular fat content and polyunsaturated fatty acid proportion in liver from lean pigs
Olivares, A., Rey, A. I., Daza, A., et al. (2010) · Livestock Science · doi:10.1016/j.livsci.2010.09.023 · Cited by 14
Composition and textural properties of Mozzarella cheese naturally-enriched in polyunsaturated fatty acids
Caroprese, M., Sevi, A., Marino, R., Santillo, A., Tateo, A., & Albenzio, M. (2013) · Journal of Dairy Research · doi:10.1017/s002202991300023x · Cited by 15
Fatty Acid Composition of Longissimus dorsi Muscle of Suffolk Down Lambs Fed on Different Dryland Forages
Gallardo, M., Pulido, R. G., & Gallo, C. (2011) · Chilean journal of agricultural research · doi:10.4067/s0718-58392011000400011 · Cited by 14
Unexpected products of the hypochlorous acid-induced oxidation of oleic acid: A study using high performance thin-layer chromatographyelectrospray ionization mass spectrometry
Schröter, J., Griesinger, H., Reuÿ, E., Schulz, M., Riemer, T., Süÿ, R., Schiller, J., & Fuchs, B. (2015) · Journal of Chromatography A · doi:10.1016/j.chroma.2015.11.059 · Cited by 14
New method to determine free sterols/oxysterols in food matrices using gas chromatography and ion trap mass spectrometry (GC–IT-MS)
Szterk, A., & Pakuła, L. (2016) · Talanta · doi:10.1016/j.talanta.2016.01.049 · Cited by 14
Induced hypernatraemia is protective in acute lung injury
Bihari, S., Dixon, D., Lawrence, M. D., et al. (2016) · Respiratory Physiology & Neurobiology · doi:10.1016/j.resp.2016.03.002 · Cited by 14
Effects of drying methods and enzyme aided on the fatty acid profiles and lipid oxidation of rice by-products
Wanyo, P., Meeso, N., Kaewseejan, N., et al. (2015) · Drying Technology · doi:10.1080/07373937.2015.1087407 · Cited by 14
Topical Application of Fingolimod Perturbs Cutaneous Inflammation
Sun, W. Y., Dimasi, D. P., Pitman, M. R., Zhuang, Y., Heddle, R. J., Pitson, S. M., Grimbaldeston, M. A., & Bonder, C. S. (2016) · The Journal of Immunology · doi:10.4049/jimmunol.1501510 · Cited by 14
Trans Fatty Acid Analyses in Samples of Marine Origin: The Risk of False Positives
Mjøs, S. A., & Haugsgjerd, B. O. (2011) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf104156v · Cited by 16
Effect of Fe3+ on the growth and lipid content of Isochrysis galbana
Liu, Z., & Wang, G. (2014) · Chinese Journal of Oceanology and Limnology · doi:10.1007/s00343-014-3110-x · Cited by 14
Effect of light intensity on the total lipid and fatty acid composition of six strains of marine diatoms
Ying, L., Kangsen, M., Shi-chun, S., & Yu, D. (2001) · Chinese Journal of Oceanology and Limnology · doi:10.1007/bf02850662 · Cited by 14
Temperature- and Time-Dependent Changes in the Structure and Composition of Glycolipids during the Growth of the Green Sulfur Photosynthetic Bacterium Chlorobaculum tepidum
Mizoguchi, T., Yoshitomi, T., Harada, J., et al. (2011) · Biochemistry · doi:10.1021/bi2002339 · Cited by 14
Does Whole-Body Hypothermia in Neonates with Hypoxic–Ischemic Encephalopathy Affect Surfactant Disaturated-Phosphatidylcholine Kinetics?
Nespeca, M., Giorgetti, C., Nobile, S., et al. (2016) · PLoS ONE · doi:10.1371/journal.pone.0153328 · Cited by 14
HPTLC‐MALDI MS for (glyco)sphingolipid multiplexing in tissues and blood: A promising strategy for biomarker discovery and clinical applications
Torretta, E., Fania, C., Vasso, M., & Gelfi, C. (2016) · Electrophoresis · doi:10.1002/elps.201600094 · Cited by 16
Applicability of traditional and advanced methods for oxidative quality and stability on marine phospholipids
Mozūraitytė, R., Kristinová, V., Standal, I. B., Evensen, H., & Rustad, T. (2016) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.201600103 · Cited by 15
Lampropedia cohaerens sp. nov., a biofilm-forming bacterium isolated from microbial mats of a hot water spring, and emended description of the genus Lampropedia
Tripathi, C., Mahato, N. K., Singh, A. K., et al. (2015) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.000853 · Cited by 14
Changes in the membrane fatty acid composition in Anoxybacillus flavithermus subsp. yunnanensis E13T as response to solvent stress
Peng, H., Yi, L., Zhang, X., Xiao, Y., Gao, Y. F., & He, C. J. (2016) · Archives of Microbiology · doi:10.1007/s00203-016-1266-9 · Cited by 13
High cell density production of multimethyl-branched long-chain esters in Escherichia coli and determination of their physicochemical properties
Menéndez-Bravo, S., Roulet, J., Sabatini, M., Comba, S., Dunn, R. O., Gramajo, H. C., & Arabolaza, A. L. (2016) · Biotechnology for Biofuels · doi:10.1186/s13068-016-0631-x · Cited by 14
Synergistic Effect of Nickel and Mercury on Fatty Acid Composition in the Muscle of Fish Lates calcarifer
Senthamils, D., Chezhian, A., & Suresh, E. (2015) · Journal of Fisheries and Aquatic Science · doi:10.3923/jfas.2016.77.84 · Cited by 14
Preservatives and sample preparation in stable isotope analysis of New Zealand freshwater invertebrates
Hogsden, K. L., & McHugh, P. A. (2016) · New Zealand Journal of Marine and Freshwater Research · doi:10.1080/00288330.2016.1257996 · Cited by 14
Studies of Lipid Monolayers Prepared from Native and Model Plant Membranes in Their Interaction with Zearalenone and Its Mixture with Selenium Ions
Gzyl-Malcher, B., Filek, M., Rudolphi-Skórska, E., et al. (2017) · The Journal of Membrane Biology · doi:10.1007/s00232-017-9958-x · Cited by 14
Antihyperlipidemic activity of Cassia auriculata flower extract in oleic acid induced hyperlipidemia in Saccharomyces cerevisiae
Vijayakumar, R., Krishnegowda, A., & Nachiappan, V. (2017) · Journal of Food Science and Technology · doi:10.1007/s13197-017-2735-0 · Cited by 14

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks