Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
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Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Kumagai, K., Sakai, S., Ueno, M., et al. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232314697 · Cited by 13
“The lipids were extracted using the Bligh and Dyer method [42] followed by separation on TLC plates (solvent system: methyl acetate/npropanol/chloroform/methanol/0.”
Method
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Lipid metabolism in dopaminergic neurons influences light entrainment
Fernandez, R. F., Wilson, E. S., Diaz, V. D., Martínez‐Gardeazabal, J., Foguth, R. M., Cannon, J. R., Jackson, S. N., Hermann, B. P., Eells, J. B., & Ellis, J. M. (2023) · Journal of Neurochemistry · doi:10.1111/jnc.15793 · Cited by 13
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Partanen, M., Honkapää, K., Hiidenhovi, J. A., Kakko, T., Mäkinen, S., Kivinen, S., Aitta, E., Väkeväinen, K., & Aisala, H. (2023) · Foods · doi:10.3390/foods12050966 · Cited by 13
“The collagen sample, due to its extremely low lipid content, was extracted using a Bligh and Dyer [13] method, as reported by Ozogul et al.”
Method
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Ulleberg, D., Sletten, I. B., Jakobsen, A. N., et al. (2023) · LWT · doi:10.1016/j.lwt.2023.114673 · Cited by 13
The Impact of MOSE (Experimental Electromechanical Module) Flood Barriers on Microphytobenthic Community of the Venice Lagoon
Baldassarre, L., Natali, V., Pascale, F. D., et al. (2023) · Microorganisms · doi:10.3390/microorganisms11040936 · Cited by 13
“Lipids (C-LIP) were analyzed following the method proposed by [43] and modified for sediments.”
Method
Rosmarinus officinalis L. Essential Oils Impact on the Microbiological and Oxidative Stability of Sarshir (Kaymak)
Hashemi, S. M. B., Gholamhosseinpour, A., & Barba, F. J. (2023) · Molecules · doi:10.3390/molecules28104206 · Cited by 17
“Peroxide Value (PV) and Anisidine Value (AnV) Determination First, Sarshir lipid was extracted using a mixture of methanol/chloroform (1:1, v/v) according to the method of Bligh and Dyer [54].”
Method
Comprehensive Treatment Strategy for Banana Inflorescence Bract to Synthesize Biodiesel and Bioethanol Through Fungal Biorefinery
Bharathi, S. D., & Jacob, S. (2023) · Waste and Biomass Valorization · doi:10.1007/s12649-023-02166-9 · Cited by 13
“After the fermentation period, the total lipid produced by the biomass grown in five different media was extracted using the Bligh and Dyer protocol [34] with few modifications.”
Assessing the potential of Chlorella sp. phycoremediation liquid digestates from brewery wastes mixture integrated with bioproduct production
Wang, S., Zhao, Q., Yu, H., Du, X., Zhang, T., Sun, T., & Song, W. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1199472 · Cited by 14
“The lipid of Chlorella sp. was extracted using the method of Bligh and Dyer (Bligh and Dyer, 1959).”
Method
The effect of immunocastration of male and female Duroc pigs on the morphological, mechanical and compositional characteristics of pork belly
Font‐i‐Furnols, M., Albano‐Gaglio, M., Brun, A., et al. (2023) · Meat Science · doi:10.1016/j.meatsci.2023.109263 · Cited by 13
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Jovanovichs, M. R. C., Pinton, M. B., Correa, L. P., et al. (2023) · Foods · doi:10.3390/foods12132439 · Cited by 13
“Chemical Composition The lipid content was determined using the method described by Bligh and Dyer [12].”
MethodInfluential
The small molecule fibroblast growth factor receptor inhibitor infigratinib exerts anti‐inflammatory effects and remyelination in a model of multiple sclerosis
Rajendran, R., Rajendran, V., Böttiger, G., Stadelmann, C., Shirvanchi, K., von Au, L., Bhushan, S., Wallendszus, N., Schunin, D., Westbrock, V., et al. (2023) · British Journal of Pharmacology · doi:10.1111/bph.16186 · Cited by 14
“Wet weight of 5 mg of tissues were homogenized with Precellys ® 24 tissue homogenizer from Bertin Instruments (Berlin, Germany) using ceramic beads and lipids were extracted according to the method of Bligh and Dyer (Bligh & Dyer, 1959).”
Method
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Hwang, H. M., Kawasawa, Y. I., Basha, A., Mohammad, S., Ito, M., & Hashimoto‐Torii, K. (2023) · Communications Biology · doi:10.1038/s42003-023-05127-z · Cited by 13
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“Total lipids were extracted from cell pellets with the use of the Bligh and Dyer method [79].”
Method
O-GlcNAc transferase acts as a critical nutritional node for the control of liver homeostasis
Ortega-Prieto, P., Parlati, L., Benhamed, F., Régnier, M., Cavalcante, I. P., Montabord, M., Onifarasoaniaina, R., Favier, M., Pavlovic, N., Magusto, J., et al. (2023) · JHEP Reports · doi:10.1016/j.jhepr.2023.100878 · Cited by 14
The effects of superheated steam roasting on proximate analysis, antioxidant activity, and oil quality of black seed (Nigella sativa)
Harivaindaran, K. V., Tiến, N. H., Đinh, T. N. S., et al. (2023) · Food Science & Nutrition · doi:10.1002/fsn3.3655 · Cited by 13
“Oil extraction was done via solvent extraction with 30 g of ground black seeds, according to Bligh & Dyer ( 1959 ), with some modifica - tions.”
Method
Extraction of chia and okra mucilages: physicochemical characterization, bioactive compounds, and antioxidant activity, drying kinetics, and thermodynamic properties
dos Santos, F. S., de Figueirêdo, R. M. F., Queiroz, A. J. D. M., et al. (2023) · Journal of Food Measurement & Characterization · doi:10.1007/s11694-023-02127-9 · Cited by 13
“Lipid determination was executed via the Bligh and Dyer method [20], a widely recognized technique for fat quantification.”
Method
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Hussain, T., Athanasiou, D., Rao, B. A., et al. (2023) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2023.167444 · Cited by 13
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“The lipid was extracted using the Bligh and Dyer method [13] with a 50:100:50 mixture of chloroform, methanol, and distilled water.”
MethodInfluential
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Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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