Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 31,501–31,600 of 48,846

Impact of Fatty-Acid Labeling of Bacillus subtilis Membranes on the Cellular Lipidome and Proteome
Nickels, J. D., Poudel, S., Chatterjee, S., Farmer, A. T., Cordner, D., Campagna, S. R., Giannone, R. J., Hettich, R. L., Myles, D. A. A., Standaert, R. F., et al. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.00914 · Cited by 13
“subtilis and Fatty Acid Methyl Ester Analysis (FAME) Total lipid extraction was performed on whole cells using a modified Bligh and Dyer method (Bligh and Dyer, 1959; Lewis et al., 2000).”
Method
Adriatic cyanobacteria potential for cogeneration biofuel production with oil refinery wastewater remediation
Haberle, I., Hrustić, ⎜., Petrić, I. S., Pritišanac, E., Šilović, T., Magić, L., Geček, S., Budiša, A., & Blažina, M. (2020) · Algal Research · doi:10.1016/j.algal.2020.101978 · Cited by 13
Effect of Media Composition and Culture Time on the Lipid Profile of the Green Microalga Coelastrum sp. and Its Suitability for Biofuel Production
Valdez-Ojeda, R. A., Serrano-Vázquez, M. G. D. R., Toledano-Thompson, T., et al. (2020) · BioEnergy Research · doi:10.1007/s12155-020-10160-5 · Cited by 13
Aphid Feeding Induces Phytohormonal Cross-Talk without Affecting Silicon Defense against Subsequent Chewing Herbivores
Johnson, S. N., Rowe, R. C., & Hall, C. R. (2020) · Plants · doi:10.3390/plants9081009 · Cited by 13
“The phytohormones salicyclic acid (SA) and jasmonic acid (JA) were extracted using the Bligh-Dyer method [59] to remove interfering compounds from the plant matrix.”
Method
Defining Caenorhabditis elegans as a model system to investigate lipoic acid metabolism
Lavatelli, A., de Mendoza, D., & Mansilla, M. C. (2020) · Journal of Biological Chemistry · doi:10.1074/jbc.ra120.013760 · Cited by 13
“Lipid extraction was performed using the Bligh and Dyer method (67).”
Method
Fatty acid nitroalkenes inhibit the inflammatory response to bleomycin-mediated lung injury
Wilkinson, M. L., Абрамова, Е. В., Guo, C., Gow, J. G., Murray, A., Koudelka, A., Cechova, V., Freeman, B. Α., & Gow, A. J. (2020) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2020.115236 · Cited by 13
Growth Performance and Organoleptic Quality of Hybrid Grouper (Epinephelus fuscogutattus ♀ × Epinephelus lanceolatus ♂) Fed Palm-Oil Based Diets at Grow-Out Stage
Gudid, S. N., Lim, L., Yong, S. K. A., et al. (2020) · Sains Malaysiana · doi:10.17576/jsm-2020-4907-09 · Cited by 13
The Beneficial Effects of Apical Sodium‐Dependent Bile Acid Transporter Inactivation Depend on Dietary Fat Composition
van de Peppel, I. P., Rao, A., Dommerholt, M. B., Bongiovanni, L., Thomas, R., de Bruin, A., Karpen, S. J., Dawson, P. A., Verkade, H. J., & Jonker, J. W. (2020) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.202000750 · Cited by 11
Impact of gamma-irradiated silver nanoparticles biosynthesized from Pseudomonas aeruginosa on growth, lipid, and carbohydrates of Chlorella vulgaris and Dictyochloropsis splendida.
Shanab, S. M. M., Partila, A. M., Ali, H. E. A., & Abdullah, M. A. (2020) · Journal of Radiation Research and Applied Sciences · doi:10.1080/16878507.2020.1856599 · Cited by 13
“Lipid was extracted using a mixture of chloroform: methanol: deionized water (1:1:0.9) ratios according to Bligh and Dyer (1959).”
MethodInfluential
Fatty Acid Composition of the Lipids from Atlantic Salmon—Comparison of Two Extraction Methods without Halogenated Solvents
Nechev, J. T., Edvinsen, G. K., & Eilertsen, K. (2021) · Foods · doi:10.3390/foods10010073 · Cited by 13
“However, we are aware that further research is needed to compare both methods in detail with the established Golden standards in lipid chemistry—Folch and Bligh and Dyer.”
MethodInfluential
Enhanced Reverse Osmosis Concentrate Disposal and Nutrients Conversion to Microalgae Bioenergy with a Gradient-Fed Strategy
Hu, R., Chang, H., Zou, Y., Feng, H., Wu, H., Wu, W., Zhong, N., Quan, X., Zhao, S., Yang, J., et al. (2021) · ACS Sustainable Chemistry & Engineering · doi:10.1021/acssuschemeng.0c07696 · Cited by 13
Improving rehabilitation outcomes using metabolomics: Health, recovery and biomarkers of mortality in sick and injured green turtles (Chelonia mydas)
Melvin, S. D., March, D. T., Marshall, K., Carroll, A. R., & van de Merwe, J. P. (2021) · Biological Conservation · doi:10.1016/j.biocon.2020.108943 · Cited by 13
Antiarthritic, anti-inflammatory activity of Moringa peregrina seed oil and leaves in Freund’s complete adjuvant-induced arthritis in rats
Shamlan, G. H., Al-Nouri, D. M., Alathbah, A. A., et al. (2021) · Journal of King Saud University - Science · doi:10.1016/j.jksus.2021.101350 · Cited by 13
Visible/Short-wave near-infrared hyperspectral analysis of lipid concentration and fatty acid unsaturation of Scenedesmus obliquus in situ
Chu, B., Chen, K., Pan, X., Wu, Q., Liu, S., Gong, J., & Li, X. (2021) · Computers and Electronics in Agriculture · doi:10.1016/j.compag.2021.106046 · Cited by 13
High VHL Expression Reverses Warburg Phenotype and Enhances Immunogenicity in Kidney Tumor Cells
Zhu, S., Ding, W., Chen, Y., Wang, W., Xu, R., Liu, C., Liu, X., & Deng, H. (2021) · Genomics Proteomics & Bioinformatics · doi:10.1016/j.gpb.2019.12.002 · Cited by 12
“According to Bligh and Dyer’s method, lipids were extracted from cells [41].”
Proteome of milk fat globule membrane and mammary gland tissue in goat fed different lipid supplementation
Marques, I. T. O., Vasconcelos, F. R., Alves, J. P. M., Montenegro, A. R., Fernandes, C. C. L., de Oliveira, F. B. B., Silva, C. P., Nagano, C. S., Figueiredo, F. C., Beserra, F. J., et al. (2021) · Small Ruminant Research · doi:10.1016/j.smallrumres.2021.106378 · Cited by 11
Isolation and characterization of docosahexaenoic acid-producing novel strain Aurantiochytrium sp. ICTFD5: A sterol with vitamin d-cholecalciferol, and cellulase and lipase producing thraustochytrid
Bagul, V. P., & Annapure, U. S. (2021) · Bioresource Technology Reports · doi:10.1016/j.biteb.2021.100688 · Cited by 13
Limits on performance and survival of juvenile sockeye salmon (Oncorhynchus nerka) during food deprivation: a laboratory-based study
Wilson, S. M., Robinson, K. A., Gutzmann, S. B., Moore, J., & Patterson, D. (2021) · Conservation Physiology · doi:10.1093/conphys/coab014 · Cited by 10
Farmed mussels (Mytilus galloprovincialis) from the Black Sea reveal seasonal differences in their neutral and polar lipid fatty acids profile
Panayotova, V., Merdzhanova, A., Stancheva, R., et al. (2021) · Regional Studies in Marine Science · doi:10.1016/j.rsma.2021.101782 · Cited by 13
Determination of long-chain fatty acids in anaerobic digester supernatant and olive mill wastewater exploiting an in-syringe dispersive liquid-liquid microextraction and derivatization-free GC-MS method
Vargas-Muñoz, M. A., Cerdà, V., Palomino, G. T., & Palacio, E. (2021) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-021-03338-z · Cited by 13
Fatty Acid Prediction in Bovine Milk by Attenuated Total Reflection Infrared Spectroscopy after Solvent-Free Lipid Separation
Akhgar, C. K., Nürnberger, V., Nadvornik, M., et al. (2021) · Foods · doi:10.3390/foods10051054 · Cited by 13
“Classical solvent–solvent extraction methods [27,28] are considered to be reliable for the quantitative separation of lipids from food and animal tissues.”
Background
Effects of a high-γ-polyglutamic acid-containing natto diet on liver lipids and cecal microbiota of adult female mice
Tamura, M., Watanabe, J., Hori, S., Atsuko, I., Yuji, K. U. B. O., Noguchi, T., Nishikawa, T., Ikezawa, M., Araki, R., & Kobori, M. (2021) · Bioscience of Microbiota Food and Health · doi:10.12938/bmfh.2020-061 · Cited by 14
“Fecal lipids were extracted from the fecal powder according to the Bligh and Dyer method [17].”
Method
Pre-breeding Diets in the Seahorse Hippocampus reidi: How Do They Affect Fatty Acid Profiles, Energetic Status and Histological Features in Newborn?
Planas, M., Olivotto, I., González, M. J., et al. (2021) · Frontiers in Marine Science · doi:10.3389/fmars.2021.688058 · Cited by 13
Evaluation of the comprehensive feeding strategy and trophic role of overexploited mesopredator species in the Sea of Marmara (northeastern Mediterranean)
Gül, G., & Demirel, N. (2021) · Estuarine Coastal and Shelf Science · doi:10.1016/j.ecss.2021.107448 · Cited by 13
Trophic structures of artificial reef communities off the southern coast of the Korean peninsula as determined using stable isotope analyses
Kang, H. Y., Lee, B., Park, H. J., Yun, S.-G., & Kang, C. (2021) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2021.112474 · Cited by 13
Fatty acid composition and lipid profiles as chemotaxonomic markers of phytopathogenic fungi Puccinia malvacearum and P. glechomatis
Wołczańska, A., Christie, W. W., Fuchs, B., et al. (2021) · Fungal Biology · doi:10.1016/j.funbio.2021.05.008 · Cited by 13
Spirulina liquid extract prevents metabolic disturbances and improves liver sphingolipids profile in hamster fed a high-fat diet
Kasbi-Chadli, F., Coué, M., Aguesse, A., Grit, I., Souque, T., Ferchaud‐Roucher, V., & Ouguerram, K. (2021) · European Journal of Nutrition · doi:10.1007/s00394-021-02589-x · Cited by 14
“Shortly, 80–90 mg of liver were homogenized in 500 μL of physiologic serum (Lyser II Qiagen) and 200 μL served for sphingolipid extraction using acidified Bligh and Dyer method [26] and purified on NH2 SPE cartridges to separate distinct sphingolipid classes.”
Method
Bioremediation of ossein effluents using the filamentous marine cyanobacterium Cylindrospermum stagnale
Ameén, F., Al-Homaidan, A. A., Alsamhary, K. I., Al-Enazi, N. M., & AlNadhari, S. (2021) · Environmental Pollution · doi:10.1016/j.envpol.2021.117507 · Cited by 13
Pilot scale production of functional foods using red palm olein: Antioxidant, vitamins’ stability and sensory quality during storage
Alkandari, S., Al-Hassawi, F., Aldughpassi, A., et al. (2021) · Saudi Journal of Biological Sciences · doi:10.1016/j.sjbs.2021.06.032 · Cited by 13
Fetal programming by androgen excess impairs liver lipid content and PPARg expression in adult rats
Silva, A. F., Abruzzese, G. A., Ferrer, M. J., Heber, M. F., Ferreira, S. R., Cerrone, G. E., & Motta, A. B. (2021) · Journal of Developmental Origins of Health and Disease · doi:10.1017/s2040174421000416 · Cited by 13
A Novel Bioanalytical Method for Determination of Inotodiol Isolated from Inonotus Obliquus and Its Application to Pharmacokinetic Study
Kim, J. H., Gao, D., Cho, C. W., Hwang, I., Kim, H. M., & Kang, J. S. (2021) · Plants · doi:10.3390/plants10081631 · Cited by 13
“The Bligh and Dyer method and the Folch method have been used to extract sterols [11,12].”
Method
Behavioural Fever Promotes an Inflammatory Reflex Circuit in Ectotherms
Sanhueza, N., Fuentes, R., Aguilar, A., Carnicero, B., Vega, K., Muñoz, D., Contreras, D., Moreno, N., Troncoso, E., Mercado, L. A., et al. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22168860 · Cited by 13
“Lipids were extracted from fish liver (3 fish per time and treatment), as described by Bligh and Dyer [78].”
Method
Assessment of Biphasic Extraction Methods of Mouse Fecal Metabolites for Liquid Chromatography–Mass Spectrometry-Based Metabolomic Studies
Yu, S., Fan, J., Zhang, L., et al. (2021) · Journal of Proteome Research · doi:10.1021/acs.jproteome.1c00450 · Cited by 13
Low light acclimation strategy of the brown macroalga Undaria pinnatifida: Significance of lipid and fatty acid remodeling for photosynthetic competence
Zhukova, N. V., & YAKOVLEVA, I. M. (2021) · Journal of Phycology · doi:10.1111/jpy.13209 · Cited by 13
Muricauda onchidii sp. nov., isolated from a marine invertebrate from South China Sea, and transfers of Flagellimonas algicola, Flagellimonas pacifica and Flagellimonas maritima to Muricauda algicola comb. nov., Muricauda parva nom. nov. and Muricauda aurantiaca nom. nov., respectively, and emended description of the genus Muricauda
Liang, J., Yin, Q., Zheng, X., et al. (2021) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.004982 · Cited by 13
Perinatal Administration of C-Phycocyanin Protects Against Atherosclerosis in apoE-Deficient Mice by Modulating Cholesterol and Trimethylamine-N-Oxide Metabolisms
Coué, M., Croyal, M., Habib, M., Castellano, B., Aguesse, A., Grit, I., Gourdel, M., Billard, H., Lépine, O., Michel, C., et al. (2021) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.121.316848 · Cited by 13
Diel biochemical and photosynthetic monitorization of Skeletonema costatum and Phaeodactylum tricornutum grown in outdoor pilot-scale flat panel photobioreactors
Maia, I. B., Carneiro, M., Magina, T., Malcata, F. X., Otero, A., Navalho, J., Varela, J. C. S., & Pereira, H. (2021) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2021.11.008 · Cited by 14
Characterization of 1-acyl-sn-glycerol-3-phosphate acyltransferase from a polyunsaturated fatty acid-producing bacterium, Shewanella livingstonensis Ac10
Cho, H.-N., Kasai, W., & Kawamoto, J. (2012) · 微量栄養素研究 · Cited by 13
Goji Berry (Lycium Barbarum L.) Carotenoids Enrichment through ‘Green’ Extraction Method Improves Oxidative Stability and Maintains Fatty Acids of Yak Ghee with Microwave Heating and Storage
Agyare, A. N., An, C. H., & Liang, Q. (2022) · Foods · doi:10.3390/foods11030369 · Cited by 13
“FAME Preparation For the pretreatment of the ghee samples and the preparation of fatty acid methyl esters (FAME), the procedure by Bligh and Dyer [27] was followed with slight modifications.”
Method
The Effect of Moringa Oleifera Leaves on Chicken Meat Composition and Meat Quality
(2022) · International Journal of Veterinary Science · doi:10.47278/journal.ijvs/2021.094 · Cited by 13
Assessment of the diagnostic and prognostic relevance of ACAT1 and CE levels in plasma, peritoneal fluid and tumor tissue of epithelial ovarian cancer patients - a pilot study
Ayyagari, V. N., Li, M., Pasman, Z., Wang, X., Louis, S., Díaz‐Sylvester, P., Groesch, K. A., Wilson, T. S., & Brard, L. (2022) · BMC Cancer · doi:10.1186/s12885-022-09476-6 · Cited by 13
“cholesterol (FC) and total cholesterol (TC) in tissue samples Tissue was extracted according to Bligh and Dyer method (1959) with modifications [21].”
Method
Positional Assignment of C−C Double Bonds in Fatty Acyl Chains of Intact Arsenosugar Phospholipids Occurring in Seaweed Extracts by Epoxidation Reactions
Coniglio, D., Ventura, G., Calvano, C. D., Losito, I., & Cataldi, T. R. I. (2022) · Journal of the American Society for Mass Spectrometry · doi:10.1021/jasms.2c00006 · Cited by 13
Application of soluble gas stabilization technology on ready‐to‐eat pre‐rigor filleted Atlantic salmon ( Salmo salar L.)
Jakobsen, A. N., Gabrielsen, L., Johnsen, E. M., et al. (2022) · Journal of Food Science · doi:10.1111/1750-3841.16164 · Cited by 13
“Raw material (n = 5) composition was assessed by measuring fat (according to Bligh & Dyer, 1959) and water content (according to ISO.6496 (1983)), whereas the residual content was assumed to be protein and ash.”
Biotechnological Potential of a New Strain of Cylindrotheca Fusiformis Producing Fatty Acids and Fucoxanthin
Gevorgiz, R. G., Gontcharov, A. A., Zheleznova, S. N., Malakhova, L. V., Alyomova, T. E., Maoka, T., & Nekhoroshev, M. V. (2022) · Bioresource Technology Reports · doi:10.1016/j.biteb.2022.101098 · Cited by 13
Lipid extraction has tissue‐dependent effects on isotopic values (δ 34 S, δ 13 C, and δ 15 N) from different marine predators
Riverón, S., Raoult, V., Slip, D. J., et al. (2022) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.9346 · Cited by 13
“The methanol phase retains 1% of total lipid content, which could include a fraction with proteins attached.(39,78) Because amino acids can differ in isotopic”
Background
Application of stable isotope analysis to evaluate the assimilation of protein sources in juvenile slipper lobsters (Thenus australiensis)
Wirtz, A., Mazumder, D., Carter, C. G., et al. (2022) · Aquaculture · doi:10.1016/j.aquaculture.2022.738570 · Cited by 13
Paenibacillus oleatilyticus sp. nov., isolated from soil
Chauhan, N. S., Joseph, N., Shaligram, S., et al. (2022) · Archives of Microbiology · doi:10.1007/s00203-022-03116-0 · Cited by 13
“The culture harvesting was done at their exponential phase of growth and the freeze-dried pellet was used for polar lipid extraction with methanol/ chloroform/saline (2:1:0.8, by vol.) as described by Bligh and Dyer (1959) considering the modifications of Card (1973).”
MethodInfluential
Comparative Study of Bioactive Lipid Extraction from Squid (Doryteuthis gahi) by-Products by Green Solvents
Aubourg, S. P., Trigo, M., González, M. J., Lois, S., & Medina, I. (2022) · Foods · doi:10.3390/foods11152188 · Cited by 14
Structural determination of the lipid A from the deep-sea bacterium Zunongwangia profunda SM-A87: a small-scale approach
Pither, M. D., Sun, M., Speciale, I., Silipo, A., Zhang, Y., Molinaro, A., & Lorenzo, F. D. (2022) · Glycoconjugate Journal · doi:10.1007/s10719-022-10076-6 · Cited by 13
Unbalanced Expression of Glutathione Peroxidase 4 and Arachidonate 15-Lipoxygenase Affects Acrosome Reaction and In Vitro Fertilization
Soria-Tiedemann, M., Michel, G., Urban, I., Aldrovandi, M., O’Donnell, V. B., Stehling, S., Kühn, H., & Borchert, A. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23179907 · Cited by 13
“Determination of the PUFA composition of sperm lipids—Sperm were prepared as described above and total sperm lipids were extracted with the Bligh–Dyer method [85].”
Method
Solid and Liquid Surface-Supported Bacterial Membrane Mimetics as a Platform for the Functional and Structural Studies of Antimicrobials
Li, S., Ren, R., Lyu, L., et al. (2022) · Membranes · doi:10.3390/membranes12100906 · Cited by 13
Seasonal variations in biochemical (biomolecular and amino acid) compositions and protein quality of particulate organic matter in the Southwestern East/Japan Sea
Jo, N., Youn, S., Joo, H., Jang, H. K., Kim, Y., Park, S., Kim, J., Kim, K., Kang, J. J., & Lee, S. H. (2022) · Frontiers in Marine Science · doi:10.3389/fmars.2022.979137 · Cited by 12
Chemical and physical characterization of spray dried fish oil with different combination ratios of wall component
Özyurt, G., Sakarya, Y., & Durmuş, M. (2022) · Journal of Food Processing and Preservation · doi:10.1111/jfpp.17223 · Cited by 13
Blackberry jam fruit (Randia formosa (Jacq.) K. Schum): An Amazon superfruit with in vitro neuroprotective properties
Silva, G. N. D. M. E., Rodrigues, E. S. B., de Macêdo, I. Y. L., et al. (2022) · Food Bioscience · doi:10.1016/j.fbio.2022.102084 · Cited by 13
Differential metabolism between biofilm and suspended Pseudomonas aeruginosa cultures in bovine synovial fluid by 2D NMR-based metabolomics
Leggett, A., Li, D., Bruschweiler‐Li, L., et al. (2022) · Scientific Reports · doi:10.1038/s41598-022-22127-x · Cited by 13
“transferred to a 50 mL conical tube and 1:1:1 methanol / ddH2O / chloroform (Fisher) was added 398 for a total volume of 24 mL (48, 49).”
Method
Meat Quality and Muscle Tissue Proteome of Crossbred Bulls Finished under Feedlot Using Wet Distiller Grains By-Product
Baldassini, W. A., Gagaoua, M., Santiago, B. M., et al. (2022) · Foods · doi:10.3390/foods11203233 · Cited by 13
A ‘Mediterranean ice-cream’: Sensory and nutritional aspects of replacing milk cream with extra virgin olive oil
Tagliamonte, S., Luca, L. D., Donato, A., Paduano, A., Balivo, A., Genovese, A., Romano, R., Vitaglione, P., & Sacchi, R. (2023) · Journal of Functional Foods · doi:10.1016/j.jff.2023.105470 · Cited by 13
Impact of dietary fat levels and fatty acid composition on milk fat synthesis in sows at peak lactation
Li, Z., Krogh, U., Lauridsen, C., Nielsen, M. O., Fang, Z., & Theil, P. K. (2023) · Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · doi:10.1186/s40104-022-00815-y · Cited by 16
“Fatty acids in oils, diets, and milk were extracted, saponified, and esterified from samples according to the method described previously [18, 22].”
Method
Delta-6 desaturase (Fads2) deficiency alters triacylglycerol/fatty acid cycling in murine white adipose tissue
Wang, C., Hucik, B., Sarr, O., Brown, L. H., Wells, K. R. D., Brunt, K. R., Nakamura, M., Harasim‐Symbor, E., Chabowski, A., & Mutch, D. M. (2023) · Journal of Lipid Research · doi:10.1016/j.jlr.2023.100376 · Cited by 13
“Lipid extraction and fractionation protocols were adapted from previously reported studies (29, 30).”
Method
The conventional microalgal biofuel production process and the alternative milking pathway: A review
Grira, S., Khalifeh, H. A., AlKhedher, M. A., & Ramadan, M. (2023) · Energy · doi:10.1016/j.energy.2023.127547 · Cited by 14
A High-Quality Genome-Scale Model for Rhodococcus opacus Metabolism
Roell, G. W., Schenk, C., Anthony, W., Carr, R. R., Ponukumati, A., Kim, J., Akhmatskaya, E. V., Foston, M. B., Dantas, G. A., Moon, T. S., et al. (2023) · ACS Synthetic Biology · doi:10.1021/acssynbio.2c00618 · Cited by 13
“42 Proteins were measured with an L-8800 AAA Hitachi High-Speed Amino Acid Analyzer.”
Method
Environment-friendly resistive memory based on natural casein: Role of electrode and bio-material concentration
Saha, M., Dey, S., Nawaz, S. M., et al. (2023) · Organic Electronics · doi:10.1016/j.orgel.2023.106869 · Cited by 13
Effects of Moringa oleifera Seed Oil on Cultured Human Sebocytes In Vitro and Comparison with Other Oil Types
Zouboulis, C. C., Hossini, A. M., Hou, X., Wang, C., Weylandt, K. H., & Pietzner, A. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241210332 · Cited by 13
“Lipid extraction was performed adapted from Bligh and Dyer extraction [38] and according to established protocols [39,40].”
Method
Major fine-scale spatial heterogeneity in accumulation of gelatinous carbon fluxes on the deep seabed
Hoving, H., Boëtius, A., Dunlop, K. M., Greinert, J., Haeckel, M., Jones, D. O. B., Simon‐Lledó, E., Marcon, Y., Stratmann, T., Suck, I., et al. (2023) · Frontiers in Marine Science · doi:10.3389/fmars.2023.1192242 · Cited by 13
Lipid Composition Analysis and Characterization of Acyl Sterol Glycosides in Adzuki and Soybean Cultivars by Non-Targeted LC-MS
Gangadhara, R. M., Gowda, S. G. B., Gowda, D., Inui, K., & Hui, S. (2023) · Foods · doi:10.3390/foods12142784 · Cited by 13
Anionic lipids facilitate membrane development and protochlorophyllide biosynthesis in etioplasts
Yoshihara, A., Kobayashi, K., Kobayashi, K., et al. (2023) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiad604 · Cited by 13
“Total lipids were extracted from seedlings crushed in liquid nitrogen according to Bligh and Dyer (1959) and were separated by 1D thin layer chromatography as described (Sato et al. 2020) with a modified solvent system of acetone:toluene: methanol:water (40:15:5:2, v/v/v/v).”
Thermal Behavior and Pyrolytic Characteristics of FreshwaterScenedesmussp. Biomass
Kumar, R. R., Ramesh, D., Mutanda, T., Rawat, I., & Bux, F. (2015) · Energy Sources Part A Recovery Utilization and Environmental Effects · doi:10.1080/15567036.2011.605428 · Cited by 13
Lipidomic Profiling of Sinus Mucosa from Patients with Chronic Rhinosinusitis
Fazlollahi, F., Kongmanas, K., Tanphaichitr, N., et al. (2015) · Clinical and Translational Science · doi:10.1111/cts.12256 · Cited by 13
The influence of the obstetrical condition on canine neonatal pulmonary functional competence
Silva, L. G., Portari, G. V., Lúcio, C. F., et al. (2015) · Journal of Veterinary Emergency and Critical Care · doi:10.1111/vec.12368 · Cited by 13
Glaciimonas alpina sp. nov. isolated from alpine glaciers and reclassification of Glaciimonas immobilis Cr9-12 as the type strain of Glaciimonas alpina sp. nov.
Frasson, D., Udovičić, M., Frey, B., et al. (2015) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.000174 · Cited by 13
Digestibilidad aparente de carbohidratos y lípidos en alimentos para camarón blanco, Litopenaeus vannamei (Decapoda: Penaeidae), cultivados en diferentes salinidades
Gucić, M., Cortés‐Jacinto, E., Ricque-Marie, D., et al. (2013) · Revista de Biología Tropical · doi:10.15517/rbt.v61i3.11935 · Cited by 13
Cell kinetics, DNA integrity, differentiation, and lipid fingerprinting analysis of rabbit adipose-derived stem cells
Barretto, L. S. D. S., Lessio, C., Nakamura, A. N., et al. (2014) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-014-9782-x · Cited by 13
Assessment of the Quality and Shelf-Life in Enriched n3 PUFA Raw Beef Patties Using Dry Soybean Sprouts as Antioxidant
Romero, M. C., Garro, O. A., Romero, A., et al. (2014) · Food and Nutrition Sciences · doi:10.4236/fns.2014.57077 · Cited by 13
Reduced glycerol incorporation into phospholipids contributes to impaired intra-erythrocytic growth of glycerol kinase knockout Plasmodium falciparum parasites
Naidoo, K., & Coetzer, T. L. (2013) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2013.08.006 · Cited by 13
Changes in the fatty acid composition of symbiotic dinoflagellates from the hermatypic coral Echinopora lamellosa during adaptation to the irradiance level
Zhukova, N. V. (2007) · Russian Journal of Plant Physiology · doi:10.1134/s1021443707060076 · Cited by 13
Depolarization-induced Rapid Generation of 2-Arachidonoylglycerol, an Endogenous Cannabinoid Receptor Ligand, in Rat Brain Synaptosomes
Oka, S., Arai, S., Waku, K., et al. (2007) · The Journal of Biochemistry · doi:10.1093/jb/mvm070 · Cited by 13
Fatty acid composition of horse mackerel (Magalaspis cordyla) and croaker (Otolithes ruber)
Nazeer, R. A., & Kumar, N. S. S. (2012) · Asian Pacific Journal of Tropical Disease · doi:10.1016/s2222-1808(12)60294-1 · Cited by 13
Optimization of goldstripe sardine (Sardinella gibbosa) protein hydrolysate using Alcalase® 2.4L by response surface methodology Optimización de hidrolisato de proteína de Sardinela dorada (Sardinella gibbosa) usando Alcalase® 2.4L a través de RSM
Taheri, A., Kenari, A. A., Motamedzadegan, A., & Rezaie, M. (2011) · CyTA - Journal of Food · doi:10.1080/19476337.2010.484551 · Cited by 13
210Po/210Pb dynamics in relation to zooplankton biomass and trophic conditions during an annual cycle in northwestern Mediterranean coastal waters
Lorda, J. F., Fowler, S. W., Miquel, J., et al. (2012) · Journal of Environmental Radioactivity · doi:10.1016/j.jenvrad.2012.07.003 · Cited by 13
Klebsiellasp. strain C2A isolated from olive oil mill waste is able to tolerate and degrade tannic acid in very high concentrations
Pepi, M., Cappelli, S., Hachicho, N., et al. (2013) · FEMS Microbiology Letters · doi:10.1111/1574-6968.12136 · Cited by 13
Effects of fasting and refeeding on the metabolic functions of the turtle Kinosternon scorpioides (Linnaeus, 1766) raised in captivity
Oliveira, A. S., Candioto, C. G., Santos, D. M. S., et al. (2013) · Pesquisa Veterinária Brasileira · doi:10.1590/s0100-736x2013000800015 · Cited by 13
Características de carcaça e qualidade da carne de bovinos de corte de quatro grupos genéticos terminados em confinamento
Clímaco, S. M., Ribeiro, E. L. D. A., Mizubuti, I. Y., et al. (2011) · Revista Brasileira de Zootecnia · doi:10.1590/s1516-35982011001200025 · Cited by 13
Repositioning of zoledronic acid for breast cancer using transferrin-conjugated liposome
Choi, M., Shin, D. H., & Kim, J. (2013) · Journal of Pharmaceutical Investigation · doi:10.1007/s40005-013-0091-2 · Cited by 13
Measuring carbon isotope ratios of microphytobenthos using compound‐specific stable isotope analysis of phytol
Oakes, J. M., Revill, A. T., Connolly, R. M., et al. (2005) · Limnology and Oceanography Methods · doi:10.4319/lom.2005.3.511 · Cited by 13
Toxic Effect of Terbium Ion on Horseradish Cell
Jiang, N., Wang, L., Lu, T., et al. (2011) · Biological Trace Element Research · doi:10.1007/s12011-011-8968-2 · Cited by 13
Effect of protein and lipid levels in diets for female red claw crayfishCherax quadricarinatuson quality of offspring (juvenile), with emphasis on growth performance, biochemical composition and stress resistance to low oxygen, high ammonia and salinity
Rodríguez‐González, H., Hernández‐Llamas, A., García‐Ulloa, M., et al. (2014) · Aquaculture Nutrition · doi:10.1111/anu.12109 · Cited by 13
Comparison of Nitrofen Uptake via Water and Food and its Distribution in Tissue of Common Carp, Cyprinus carpio L.
Inoue, Y., Hashizume, N., Kikushima, E., et al. (2011) · Bulletin of Environmental Contamination and Toxicology · doi:10.1007/s00128-011-0356-2 · Cited by 13
Comparison of FA compositions of selected tissues of phocid seals of Eastern Canada using one‐way and multivariate techniques
Durnoford, E., & Shahidi, F. (2002) · Journal of the American Oil Chemists Society · doi:10.1007/s11746-002-0610-7 · Cited by 13
Comparison of Proximate Composition and Fatty Acid Profiles in Cultured and Wild Marine Fishes in Kuwait
Hossain, M. A., Almatar, S. M., Al-Abdul-Elah, K., et al. (2012) · Journal of Applied Aquaculture · doi:10.1080/10454438.2012.678790 · Cited by 13
Desmosterol replaces cholesterol for ligand binding function of the serotonin1A receptor in solubilized hippocampal membranes: Support for nonannular binding sites for cholesterol?
Singh, P., Jafurulla, M., Paila, Y. D., et al. (2011) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2011.06.022 · Cited by 13
Sphingosine Kinase-1 Protects Differentiated N2a Cells Against Beta-Amyloid25–35-Induced Neurotoxicity Via the Mitochondrial Pathway
Yang, Y., Wang, M., Lv, B., et al. (2014) · Neurochemical Research · doi:10.1007/s11064-014-1290-6 · Cited by 13
Structure of milk triacylglycerols of five marsupials and one monotreme: Evidence for an unusual pattern common to marsupials and eutherians but not found in the echidna, a monotreme
Grigor, M. R. (1980) · Comparative Biochemistry and Physiology Part B Comparative Biochemistry · doi:10.1016/0305-0491(80)90042-5 · Cited by 13
Sequestration of PDC ‐109 Protein by Specific Antibodies and Egg Yolk Cryoprotects Bull Spermatozoa
Srivastava, N., Srivastava, S., Ghosh, S. K., et al. (2013) · Reproduction in Domestic Animals · doi:10.1111/rda.12151 · Cited by 13
Molecular species of triacylglycerols from the freshwater diatom, Phaeodactylum tricornutum
Yongmanitchai, W., & Ward, O. P. (1993) · Phytochemistry · doi:10.1016/s0031-9422(00)95078-7 · Cited by 13
Opposing Effects of Reduced Kidney Mass on Liver and Skeletal Muscle Insulin Sensitivity in Obese Mice
Chin, S. H., Item, F., Wueest, S., Zhou, Z., Wiedemann, M. S. F., Gai, Z., Schoenle, E. J., Kullak-Ublick, G. A., Al‐Hasani, H., & Konrad, D. (2014) · Diabetes · doi:10.2337/db14-0779 · Cited by 12
Effect of oxidants on small intestinal brush border membranes and colonic apical membranes—a comparative study
Prabhu, R., & Balasubramanian, K. A. (2003) · Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology · doi:10.1016/s1532-0456(02)00250-8 · Cited by 13
Medium chain-length fatty acids in lipids of developing oil palm kernel endosperm
Wiberg, E., & Bafor, M. E. (1995) · Phytochemistry · doi:10.1016/0031-9422(95)00194-c · Cited by 13
A 3-week dietary bioequivalence study in preweaning farm piglets of two sources of docosahexaenoic acid produced from two different organisms
Fedorova-Dahms, I., Thorsrud, B. A., Bailey, E., & Salem, N. (2013) · Food and Chemical Toxicology · doi:10.1016/j.fct.2013.12.008 · Cited by 13
Optimisation of biomass and lipid production by adjusting the interspecific competition mode of Dunaliella salina and Nannochloropsis gaditana in mixed culture
Liu, J. (2013) · Journal of Applied Phycology · doi:10.1007/s10811-013-0099-z · Cited by 13
Cholesterol induces renal vasoconstriction and anti-natriuresis by inhibiting nitric oxide production in anesthetized rats
Kopkan, L., Khan, A. H., Lis, A., Awayda, M. S., & Majid, D. S. A. (2009) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.90743.2008 · Cited by 13

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks