Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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“Cellular neutral lipids were extracted from DCs during the differentiation process using the Bligh-Dyer method (27).”
Method
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Park, Y. H., Yu, B. S., Heo, Y. M., Kyung, S., Lee, K., Kim, S., Kang, S., Han, K., & Kim, D. H. (2024) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-024-13292-2 · Cited by 14
“Lipid accumulation in M. furfur cells was measured during the culture period under different initial pH conditions (pH 7, 6.6, 6, and 5) using a modified Bligh and Dyer method (Bligh and Dyer 1959; Yu et al. 2022).”
Influential
Modulating the techno-functional properties of quinoa (Chenopodium quinoa Wild) protein concentrate using high-pressure technologies and their impact on in vitro digestibility: A comparative study
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“Lipid extraction was conducted based on the Bligh and Dyer method as described elsewhere (39–41).”
Microglia morphological response to mesenchymal stromal cell extracellular vesicles demonstrates EV therapeutic potential for modulating neuroinflammation
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“Currently there are different techniques that use solvents, one of the most used is Bligh and Dyer (B&D) method for lipid quantitation at analytical level (Bligh and Dyer, 1959; Azmin et al., 2016).”
BackgroundMethod
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Crosstalk between mitochondrial homeostasis and AMPK pathway mediate the receptor-mediated cardioprotective effects of estradiol in ovariectomized female rats
Gowayed, M. A., Zakaraya, Z. Z., Abu‐Samra, N., Elhamammy, R. H., Moneim, L. M. A., Hafez, H. A., Moneam, I. A., Oriquat, G. A., & Kamel, M. A. E.-N. (2024) · PLoS ONE · doi:10.1371/journal.pone.0312397 · Cited by 13
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Ballon, A., Queiroz, L. S., Castellví, S. D. L., et al. (2025) · Food Chemistry · doi:10.1016/j.foodchem.2025.143339 · Cited by 12
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“Plasma and erythrocyte lipids were extracted according to the method of Folch and Bligh and Dyer et al. [16, 17] using a methanol/chloroform/water mixture (2:1:1, v/v/v).”
Expanding the cultivable human archaeome: Methanobrevibacter intestini sp. nov. and strain Methanobrevibacter smithii ‘GRAZ-2’ from human faeces
Weinberger, V., Mohammadzadeh, R., Blohs, M., Kalt, K., Mahnert, A., Moser, S., Cecovini, M., Mertelj, P., Zurabishvili, T., Arora, B., et al. (2025) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.006751 · Cited by 15
“30 mg) using a modified Bligh and Dyer extraction as described previously [29–32].”
Lipidomic and proteomic insights from extracellular vesicles in the postmortem dorsolateral prefrontal cortex reveal substance use disorder-induced brain changes
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“In accordance with the manufacturer ’ s instructions, the base medium used was EMEM (ATCC ® 30-2003 ™ ) supplemented with 10% FBS. Human astrocytes (HAs) were purchased from Science Cell, Cat#1800.”
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A time efficient adaptation of GC-FID method for the analysis of PBMC lipid composition
Kumar, A. P. R., Lindley, M. R., & Mastana, S. S. (2014) · Loughborough University Institutional Repository (Loughborough University) · doi:10.51847/vfq2kie · Cited by 11
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Salazar-Vega, M. I., Rosado-Rubio, J. G., Chel‐Guerrero, L., Betancur‐Ancona, D., & Ruelas, A. F. C. (2009) · LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) · Cited by 12
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NONPHOSPHORUS LIPIDS IN PERIPHYTON REFLECT AVAILABLE NUTRIENTS IN THE FLORIDA EVERGLADES, USA1
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Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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