Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Yoshinaga, K., Usami, Y., Yoshinaga-Kiriake, A., Shikano, H., Taira, S., Nagasaka, R., Tanaka, S., & Gotoh, N. (2021) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2021.108897 · Cited by 12
Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin
Tarantini, F. S., Brunati, M., Taravella, A., et al. (2021) · PLoS ONE · doi:10.1371/journal.pone.0260413 · Cited by 12
“…C18 column (Macherey-Nagel, 2 mm x 125 mm, 3 μ m, RP-18) and the effluent monitored at 269 nm. Polar lipids were extracted from freeze-dried biomass (after lipoquinones extraction) using a modification of the method of Bligh and Dyer [32] and separated by two-dimensional TLC on silica gel.”
Effect of Bismuth, Gadolinium, and Cadmium Nanoparticles on Biomass, Carotenoid, and Lipid Content of Dunaliella salina (Dunal) Teodoresco
Makhi, V. V., Ahmad, A., & Chaugule, B. (2021) · Current Microbiology · doi:10.1007/s00284-021-02710-7 · Cited by 12
“Total lipid was extracted from the samples according to the previously reported method [23].”
Method
Lipid Alterations in African American Men with Prostate Cancer
Ravindran, A., Piyarathna, D. W. B., Gohlke, J., et al. (2021) · Metabolites · doi:10.3390/metabo12010008 · Cited by 12
“Briefly, a modified Bligh–Dyer method [20] was used to extract the lipids.”
Method
Effect of dietary fish oil replacement with grape seed oil on growth performance, feed utilization and fatty acid profile of mirror carp, Cyprinus carpio , fingerlings
Zorlu, K., & Gümüş, E. (2021) · Aquaculture Research · doi:10.1111/are.15704 · Cited by 12
Investigation of the quality parameters of hydrolysates obtained from fish by‐products using response surface methodology
Korkmaz, K., & Tokur, B. (2022) · Journal of Food Processing and Preservation · doi:10.1111/jfpp.16296 · Cited by 12
Halorubrum pleomorphic virus-6 Membrane Fusion Is Triggered by an S-Layer Component of Its Haloarchaeal Host
Bignon, E. A., Chou, K., Roine, E., & Tischler, N. D. (2022) · Viruses · doi:10.3390/v14020254 · Cited by 12
“8 v/v) extraction, as previously described [44].”
Application of orange albedo fat replacer in chicken mortadella
Belluco, C. Z., Mendonça, F. J., Zago, I. C. C., et al. (2022) · Journal of Food Science and Technology · doi:10.1007/s13197-022-05382-8 · Cited by 12
Respostas metabólicas e nutricionais de jovens da tilápia do Nilo a fontes dietéticas de methionina
Teodósio, R., Engrola, S. A. D., Cabano, M., et al. (2022) · Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) · Cited by 12
Maternal urinary phthalate metabolites are associated with lipidomic signatures among pregnant women in Puerto Rico
Ashrap, P., Aung, M. T., Watkins, D. J., et al. (2022) · Journal of Exposure Science & Environmental Epidemiology · doi:10.1038/s41370-022-00410-3 · Cited by 12
Effect of Hypoxia on the Function of the Human Serotonin1A Receptor
Dutta, A., Sarkar, P., Shrivastava, S. K., & Chattopadhyay, A. (2022) · ACS Chemical Neuroscience · doi:10.1021/acschemneuro.2c00181 · Cited by 12
Effect of metallic nanoparticles on microalgal growth and lipid accumulation for biodiesel production
Öztay, D., İnan, B., Koçer, A. T., & Özçimen, D. B. (2022) · Brazilian Journal of Chemical Engineering · doi:10.1007/s43153-022-00232-8 · Cited by 12
“1956), Bligh and Dyer method (Bligh and Dyer 1959) and Lowry method (Lowry et al.”
Method
Algae for Wastewater Treatment and Production of Biofuels and Bioproducts
Deng, Z., Khatiwada, J. R., Fan, L., & Qin, W. (2022) · International Journal of Environmental Research · doi:10.1007/s41742-022-00412-x · Cited by 12
“2020), mixed methanol-chloroform (2:1 v/v) (Bligh and Dyer 1959), and mixed ether-petroleum (Hidalgo et al.”
Background
Liposomes
Kalra, J., & Bally, M. B. (2013) · Springer eBooks · doi:10.1007/978-1-4614-9164-4_3 · Cited by 12
Prevention of Ulcerative Colitis by Autologous Metabolite Transfer from Colitogenic Microbiota Treated with Lipid Nanoparticles Encapsulating an Anti-Inflammatory Drug Candidate
Yang, C., Sung, J. J., Long, D., Alghoul, Z., & Merlin, D. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14061233 · Cited by 13
“We extracted the lipids from GDNPs-2 using a modified liquid–liquid extraction (LLE) method introduced by Bligh and Dyer [15,16].”
Method
Impact of low-cost diets on maturation of the red claw crayfish Cherax quadricarinatus: An integrative approach during a long-term study
Greco, L. S. L., Stumpf, L., Timpanaro, S., et al. (2022) · Aquaculture · doi:10.1016/j.aquaculture.2022.738614 · Cited by 12
Fatty Acid Production by Enhanced Malonyl-CoA Supply in Escherichia coli
Kaku, M., Ishidaira, M., Satoh, S., et al. (2022) · Current Microbiology · doi:10.1007/s00284-022-02969-4 · Cited by 12
“Cellular lipids were extracted using the Bligh–Dyer method [36].”
Method
Development of a multi-omics extraction method for ecotoxicology: investigation of the reproductive cycle of Gammarus fossarum
Faugere, J., Brunet, T. A., Clément, Y., et al. (2022) · Talanta · doi:10.1016/j.talanta.2022.123806 · Cited by 12
Modeling and experimental assessment of chemical and enzymatic extraction of bioactive components from passion fruit seed oil
Oliveira, V. V., Rosalém, M. D. J. C., Aranha, A. C. R., Sípoli, C. C., Andrade, M. M., Tonin, L. T. D., Defendi, R. O., & Suzuki, R. M. (2022) · Journal of Food Processing and Preservation · doi:10.1111/jfpp.17106 · Cited by 14
Growth and Metabolite Production in Chlorella sp.: Analysis of Cultivation System and Nutrient Reduction
Estevam, B. R., Pinto, L. F. R., Filho, R. M., & Fregolente, L. V. (2022) · BioEnergy Research · doi:10.1007/s12155-022-10532-z · Cited by 12
“Modified Bligh and Dyer’s [29] method was used for lipid extraction.”
Method
Concentration of synthetic antioxidants and peroxide value of commercial dry pet foods
Costa, J. L. G., Pedreira, R. S., Gomes, A. C. P., et al. (2022) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2022.115499 · Cited by 12
The environmental pollutant, polychlorinated biphenyl 126, alters liver function in a rodent model of alcohol‐associated liver disease
Gripshover, T. C., Wahlang, B., Head, K. Z., Young, J. L., Luo, J., Mustafa, M. T., Kirpich, I., & Cave, M. C. (2022) · Alcoholism Clinical and Experimental Research · doi:10.1111/acer.14976 · Cited by 12
Genome-Wide Identification and Expression Analysis of Fatty Acid Desaturase (FAD) Genes in Camelina sativa (L.) Crantz
Sun, D., Quan, W., Wang, D., et al. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232314550 · Cited by 12
Mirabegron-induced brown fat activation does not exacerbate atherosclerosis in mice with a functional hepatic ApoE-LDLR pathway
Ying, Z., van Eenige, R., Beerepoot, R., Boon, M. R., Kloosterhuis, N., van de Sluis, B., Bartelt, A., Rensen, P. C. N., & Kooijman, S. (2022) · Pharmacological Research · doi:10.1016/j.phrs.2022.106634 · Cited by 12
Synergistic antidepressant-like effect of n-3 polyunsaturated fatty acids and probiotics through the brain-gut axis in rats exposed to chronic mild stress
Cho, H., Jo, M., Oh, H., Lee, Y., & Park, Y. (2023) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2023.109326 · Cited by 11
Modulation of Dietary Fatty Acids in an Open-Label Study Improves Psoriasis and Dampens the Inflammatory Activation Status
Saalbach, A., Seitz, A., Kohlmann, J., Kalweit, L., Vogt, L., Selig, L., Engel, K. M., & Simon, J. C. (2023) · Nutrients · doi:10.3390/nu15071698 · Cited by 12
“Lipids were extracted from the supernatant by the addition of 1 mL chloroform and water according to Bligh and Dyer [20].”
Method
Stearoyl-CoA desaturase 1 deficiency exacerbates palmitate-induced lipotoxicity by the formation of small lipid droplets in pancreatic β-cells
Janikiewicz, J., Dobosz, A. M., Majzner, K., Bernaś, T., & Dobrzyń, A. (2023) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2023.166711 · Cited by 13

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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