Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Partial characterization of novel inulin‐like prebiotic fructooligosaccharides of Sechium edule (Jacq.) Sw. (Cucurbitaceae) tuberous roots
Bandyopadhyay, B., Mandal, V., & Mandal, N. C. (2021) · Journal of Food Biochemistry · doi:10.1111/jfbc.13764 · Cited by 9
Action of alkyl glycerol ethers and n‐3 polyunsaturated fatty acids diet on hematological parameters of blood and liver plasmalogen level in aged rats
Sultanov, R. M., Ermolenko, E. V., Poleschuk, T. S., Denisenko, Y., & Kasyanov, S. P. (2021) · Journal of Food Science · doi:10.1111/1750-3841.15756 · Cited by 9
Free Fatty Acids Reduction in Waste Cooking Oil by Rhodosporidium toruloides and Simultaneous Carotenoids, Lipids, and PAL Enzyme Production in a Two‐Phase Culture System
Lee, J. J. L., Li, A. Z., Lyu, X., et al. (2021) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.202000354 · Cited by 9
Multitargeted Approach for the Optimization of Morphogenesis and Barrier Formation in Human Skin Equivalents
Mieremet, A., Helder, R. W. J., Nădăban, A., Boiten, W. A., Gooris, G. S., Ghalbzouri, A. E., & Bouwstra, J. A. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22115790 · Cited by 8
“Lipids were extracted using a modified Bligh and Dyer extraction procedure, as reported before [19,58].”
Method
Lipid composition and molecular species of phospholipid in oyster Crassostrea lugubris (Sowerby, 1871) from Lang Co Beach, Hue Province, Vietnam
Le, T. T. T., Tran, Q. T., Velansky, P., et al. (2021) · Food Science & Nutrition · doi:10.1002/fsn3.2385 · Cited by 9
Reference measurement procedure for the determination of mass fraction of fat content in food
Medvedevskikh, S. V., Baranovskaya, V. B., Medvedevskikh, M. Y., et al. (2021) · Accreditation and Quality Assurance · doi:10.1007/s00769-021-01472-w · Cited by 9
Techno-Economic Analysis of Producing Oil Rich in ɷ-3 from Catfish Processing Wastes
Igansi, A. V., da Silva, P. P., Engelmann, J. I., Pinto, L. A. D. A., Paes, R. L., & Cadaval, T. R. S. (2021) · Waste and Biomass Valorization · doi:10.1007/s12649-021-01502-1 · Cited by 9
Nutritional quality, fatty acids content and antioxidant capacity of pecan nut fruits from Criolla and Improved walnut varieties
Curiel‐Maciel, N. F., Arreola-Avila, J. G., Esparza-Rivera, J. R., et al. (2021) · Notulae Botanicae Horti Agrobotanici Cluj-Napoca · doi:10.15835/nbha49212021 · Cited by 9
FTIR combined with chemometric tools — a potential approach for early screening of grazers in microalgal cultures
Deore, P., Beardall, J., Palacios, Y. M., Noronha, S., & Heraud, P. (2021) · Journal of Applied Phycology · doi:10.1007/s10811-021-02543-8 · Cited by 9
Antioxidative and antimicrobial effects of low molecular weight shrimp chitosan and its derivatives on seasoned‐dried Pangasius fillets
Bình, N. T. T., Bao, H. N. D., Prinyawiwatkul, W., et al. (2021) · International Journal of Food Science & Technology · doi:10.1111/ijfs.15280 · Cited by 9
Three‐liquid‐phase salting‐out extraction of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)‐rich oils from Euphausia superba
Zeb, L., Teng, X., Shafiq, M., et al. (2021) · Engineering in Life Sciences · doi:10.1002/elsc.202000098 · Cited by 9
Proof-of-concept on the effect of human milk storage time: Lipid degradation and spectroscopic characterization using portable near-infrared spectrometer and chemometrics
dos Santos, V. J., Baqueta, M. R., Março, P. H., et al. (2021) · Food Chemistry · doi:10.1016/j.foodchem.2021.130675 · Cited by 9
NMR-Based Metabolomics Approach to Investigate the Effects of Fruits of Acanthopanax sessiliflorus in a High-Fat Diet Induced Mouse Model
Choi, B. R., Yoon, D., Kim, H., Oh, S. M., Yoo, Y. C., Lee, Y., Kim, K.-W., Yi, T., & Lee, D. (2021) · Metabolites · doi:10.3390/metabo11080505 · Cited by 9
“An extraction protocol using the Bligh and Dyer method [45] was optimized in this experiment.”
Method
Effect of exogenous methyl jasmonate on in vitro propagation, metabolic profiling and proximadiol production from Cymbopogon schoenanthus subsp. proximus
Abdelsalam, A., Mahran, E., Chowdhury, K., et al. (2021) · Plant Physiology Reports · doi:10.1007/s40502-021-00608-x · Cited by 9
Bioethanol production from seagrass waste, through fermentation process using cellulase enzyme isolated from marine actinobacteria
Rajkumar, J., Dilipan, E., Ramachandran, M. S., Panneerselvam, A., & Thajuddin, N. (2021) · Vegetos · doi:10.1007/s42535-021-00239-5 · Cited by 9
“1951), and total lipid (Bligh and Dyer 1959).”
Benthic foraminiferal assemblages and environmental drivers along the Kveithola Trough (NW Barents Sea)
Caridi, F., Sabbatini, A., Bensi, M., et al. (2021) · Journal of Marine Systems · doi:10.1016/j.jmarsys.2021.103616 · Cited by 9
DPP4 inhibitor reduces portal hypertension in cirrhotic rats by normalizing arterial hypocontractility
Wang, X., Gu, H., Li, K., et al. (2021) · Life Sciences · doi:10.1016/j.lfs.2021.119895 · Cited by 9
Enhancing sunflower oil by the addition of commercial thyme and rosemary essential oils: The effect on lipid quality of Mediterranean horse mackerel and anchovy during traditional pan-frying
Tokur, B., Korkmaz, K., & Uçar, Y. (2021) · International Journal of Gastronomy and Food Science · doi:10.1016/j.ijgfs.2021.100428 · Cited by 9
Dietary Phospholipids Prepared From Scallop Internal Organs Attenuate the Serum and Liver Cholesterol Contents by Enhancing the Expression of Cholesterol Hydroxylase in the Liver of Mice
Sugimoto, K., Hosomi, R., Yoshida, M., & Fukunaga, K. (2021) · Frontiers in Nutrition · doi:10.3389/fnut.2021.761928 · Cited by 9
“Liver total lipids were extracted using by Bligh and Dyer (37) method and then dissolved in 2-propanol.”
Method
Impact of sous vide cooking on quality and shelf‐life of dried sour‐salted fish
Pongsetkul, J., & Benjakul, S. (2021) · Journal of Food Processing and Preservation · doi:10.1111/jfpp.16142 · Cited by 9
Biochemical Characterization and Storage Stability of Process Waters from Industrial Shrimp Production
Forghani, B., Sørensen, A. M., Fredeus, G., et al. (2021) · ACS Omega · doi:10.1021/acsomega.1c03304 · Cited by 9
Stomach secretes estrogen in response to the blood triglyceride levels
Ito, T., Yamamoto, Y., Yamagishi, N., & Kanai, Y. (2021) · Communications Biology · doi:10.1038/s42003-021-02901-9 · Cited by 12

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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