Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
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Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Method
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Shibata, M., Coelho, C. M. M., Steiner, N., et al. (2020) · CERNE · doi:10.1590/01047760202026022653 · Cited by 8
“Lipid content Lipid extraction followed Bligh and Dyer (1959) with modifications by Brum et al. (2009).”
Method
Tabrizicola algicola sp. nov. isolated from culture of microalga Ettlia sp.
Park, C.-Y., Chun, S.-J., Jin, C.-Z., Le, V. V., Cui, Y., Kim, S.-Y., Ahn, C., & Oh, H. (2020) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.004508 · Cited by 9
Restoration of ceramidede novosynthesis by the synthetic retinoid ST1926 as it induces adult T-cell leukemia cell death
Ghandour, B., Pisano, C., Darwiche, N., & Dbaibo, G. S. (2020) · Bioscience Reports · doi:10.1042/bsr20200050 · Cited by 8
“Ceramide measurement Lipids were collected by the method of Bligh and Dyer [49].”
Influential
Physical Characterization and Biodistribution of Cisplatin Loaded in Surfactant Modified-Hybrid Nanoparticles Using Polyethylene Oxide-b-Polymethacrylic Acid
Miatmoko, A. (2020) · Advanced Pharmaceutical Bulletin · doi:10.34172/apb.2021.086 · Cited by 8
Characterization of strains of Chlorella from Abakaliki, Nigeria, for the production of high-value products under variable temperatures
Idenyi, J. N., Eya, J. C., Ogbonna, J. C., Chia, M. A., Alam, M. A., & Ubi, B. E. (2020) · Journal of Applied Phycology · doi:10.1007/s10811-020-02313-y · Cited by 9
“The Bligh and Dyer method (Bligh and Dyer 1959) was used to extract total lipids from 2.”
MethodInfluential
Physicochemical and bioactive compounds evaluation of Physalis pubescens Linnaeus
Zimmer, T. B. R., Otero, D. M., & Zambiazi, R. C. (2020) · Revista CERES · doi:10.1590/0034-737x202067060002 · Cited by 8
Dietary supplementation with myo-inositol reduces high-fructose diet-induced hepatic ChREBP binding and acetylation of histones H3 and H4 on the Elovl6 gene in rats
Hibi, M., Nakagawa, T., Hayakawa, T., Yanase, E., & Shimada, M. (2020) · Nutrition Research · doi:10.1016/j.nutres.2020.12.022 · Cited by 10
Trimethylornithine Membrane Lipids: Discovered in Planctomycetes and Identified in Diverse Environments
Moore, E. K. (2021) · Metabolites · doi:10.3390/metabo11010049 · Cited by 8
“The peat core was divided into five sections (5 to 10 cm, 10 to 20 cm, 20 to 30 cm, 30 to 40 cm, and 40 to 50 cm) and IPLs were extracted from Obukhoskoye peat using a modified Bligh and Dyer extraction method [45,46].”
Method
Babassu Cake in Goat Diet Improves Growth Performance and Quality and Fatty Acid Profile of Meat
Oliveira, T. P. R., Júnior, J. A. A. C., Costa, J. B., et al. (2021) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.202000277 · Cited by 8
In Vitro Evaluation of Antioxidant Potential of the Invasive Seagrass Halophila stipulacea
Sansone, C., Galasso, C., Martire, M. L., Fernández, T. V., Musco, L., Dell’Anno, A., Bruno, A., Noonan, D. M., Albini, A., & Brunet, C. (2021) · Marine Drugs · doi:10.3390/md19010037 · Cited by 8
“Total lipids were extracted in chloroform: methanol (1:1, vol:vol) [68], and the resulting fraction, after evaporation in a dry hot bath at 80 to 100 ◦C for 20 min, was quantified according to the sulfuric acid carbonization procedure [69].”
Method
UHPLC-IM-Q-ToFMS analysis of maradolipids, found exclusively in Caenorhabditis elegans dauer larvae
Witting, M. A., Schmidt, U. G., & Knölker, H. (2021) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-021-03172-3 · Cited by 8
“In order to prove that this combination is able to identify maradolipids also in biological extracts, C. elegans dauer larvae were generated from daf-2(e1370) mutants by growing them at 25 °C. Worms were harvested and extracted using a Bligh and Dyer extraction.”
Method
Physicochemical and sensory evaluation of mortadella based on Nile tilapia filleting residues
Alda, P. C., Coradini, M., Chambo, A. P. S., et al. (2021) · Ciência Rural · doi:10.1590/0103-8478cr120190979 · Cited by 8
Calcium homeostasis and stable fatty acid composition underpin heatwave tolerance of the keystone polychaete Hediste diversicolor
Madeira, D., Fernandes, J. F., Jerónimo, D., Ricardo, F., Santos, A., Domingues, M. R., & Calado, R. (2021) · Environmental Research · doi:10.1016/j.envres.2021.110885 · Cited by 11
Effect of Different Drying Methods on the Nutritional Value of Hibiscus sabdariffa Calyces as Revealed by NMR Metabolomics
Marak, S., Shumilina, E., Kaushik, N., Falch, E., & Dikiy, A. (2021) · Molecules · doi:10.3390/molecules26061675 · Cited by 8
“Lipid content was determined following the method of Bligh and Dyer [42].”
Method
Metabolic Changes of Cholangiocarcinoma Cells in Response to Coniferyl Alcohol Treatment
Promraksa, B., Katrun, P., Phetcharaburanin, J., et al. (2021) · Biomolecules · doi:10.3390/biom11030476 · Cited by 8
“We performed cell extraction following the protocol of Bligh and Dyer (1959) [28].”
Method
Bioactivity of atemoya fruits and by-products
de Moraes, M. R., da Silveira, T. F. F., Coutinho, J. P., Souza, D. S., Duarte, M. C. T., Duarte, R. M. T., Filho, J. T., & Godoy, H. T. (2021) · Food Bioscience · doi:10.1016/j.fbio.2021.101036 · Cited by 8
Improving lipid production capacity of new natural oleaginous yeast: Pichia cactophila firstly
Berikten, D., Hoşgün, E. Z., Bozan, B., et al. (2021) · Biomass Conversion and Biorefinery · doi:10.1007/s13399-021-01466-1 · Cited by 8
MG@PD@TiO2 nanocomposite based magnetic solid phase extraction coupled with LC–MS/MS for determination of lysophosphatidylcholines biomarkers of plasma in psoriasis patients
He, X., Cao, H., Li, X., et al. (2021) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2021.114101 · Cited by 8
Nutritional quality variation in dried Pacific Oyster (Crassostrea gigas) using hybrid-pump dryer under different heating treatment
Neri, T. A. N., Jung, H.-C., Jang, S.-K., Kang, S.-J., & Choi, B.-D. (2021) · Food Science and Biotechnology · doi:10.1007/s10068-021-00907-x · Cited by 8

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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