Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Method
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Method
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Method
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Method
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Method
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“Select urine samples were also used for a Bligh-Dyer extraction and were mixed with a 2:1 ratio of cold chloroform/methanol, vortexed, incubated on ice (5 min), centrifuged for 5 min (max speed, 4°C), the upper layer containing lipids was resuspended in 50% cold acetonitrile in a LC vial for confirmation of LPC (22:6)…”
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Creydt, M., Flügge, F., Dammann, R., et al. (2023) · Metabolites · doi:10.3390/metabo13050673 · Cited by 8
“For the extraction of the non-polar analytes, a modified protocol from Bligh and Dyer was used, with which convincing results had already been obtained in previous studies on comparable matrices [22,23].”
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Coutinho, G. S. M., Ribeiro, A. E. C., Prado, P. M. C., et al. (2023) · Journal of Food Science and Technology · doi:10.1007/s13197-023-05781-5 · Cited by 8
Can banana peel and sweet potato vines serve as efficient feed ingredients in diets for growing rabbits?
Falcone, D. B., Klinger, A. C. K., Silva, S. S., et al. (2023) · Tropical Animal Health and Production · doi:10.1007/s11250-023-03697-y · Cited by 8
“The crude protein content, ash and fiber (neutral detergent, acid detergent and crude) in the samples of feed, according to AOAC (1995) and crude fat (Bligh and Dyer 1959) at the Fish Farming Laboratory, Department of Animal Science of UFSM (Table 2).”
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Skin Anti-Inflammatory Potential with Reduced Side Effects of Novel Glucocorticoid Receptor Agonists
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“A total of 0.5 mL HCl (0.25 M) was added to neutralize the alkaline reaction mixture.”
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Bedics, A., Táncsics, A., Banerjee, S., et al. (2024) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.006219 · Cited by 8
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Proximate composition and fatty acid profile of different tissues of the marine neogastropod Cymbium melo (Solander, 1786).
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Boudah, S., Paris, A., & Junot, C. (2013) · Advances in botanical research · doi:10.1016/b978-0-12-397922-3.00004-6 · Cited by 8
Determination of Hydroperoxides in Liposomes by the Modified IDF and the Modified Tiron Methods
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Interaction of albumin and phospholipid:cholesterol liposomes in growth of Mycoplasma spp
Cluss, R. G., & Somerson, N. L. (1986) · Applied and Environmental Microbiology · doi:10.1128/aem.51.2.281-287.1986 · Cited by 8
Fatty acid composition and biological activities of volatiles from fruits of two Tunisian olive cultivars
Brahmi, F., Dabbou, S., Flamini, G., et al. (2011) · International Journal of Food Science & Technology · doi:10.1111/j.1365-2621.2011.02616.x · Cited by 8
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Richard, D., Labrie, A., Lupien, D., et al. (1982) · Journal of Nutrition · doi:10.1093/jn/112.9.1756 · Cited by 8
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Ariyani, F., Amin, I., Fardiaz, D., et al. (2008) · Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan · doi:10.15578/jpbkp.v3i2.20 · Cited by 8
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Tanida, I., Ueno, T., & Kominami, E. (2014) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb1120s64 · Cited by 8
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Almada, M., Domingues, M. R., Dória, M. L., Fonseca, B. M., Teixeira, N. A. A., & Correia‐da‐Silva, G. (2014) · Journal of Cellular Physiology · doi:10.1002/jcp.24901 · Cited by 8
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Enssani, E. (2005) · Intersociety Energy Conversion Engineering Conference · doi:10.1109/iecec.1990.748060 · Cited by 8
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Replacing canola meal as the major protein source with wheat dried distillers’ grains alters omasal fatty acid flow and milk fatty acid composition in dairy cows
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Sensorial characteristics and fatty acid mozzarella cheese from milk of crossbred cows fed with palm oil and coconut fat - doi: 10.4025/actascitechnol.v35i4.20158
Corradini, S. A. D. S., Madrona, G. S., de Souza, N. E., et al. (2013) · Acta Scientiarum. Technology/Acta scientiarum. Technology · doi:10.4025/actascitechnol.v35i4.20158 · Cited by 8
Brain lipid changes after repetitive transcranial magnetic stimulation: potential links to therapeutic effects?
Lee, L. H.-W., Tan, C. H., Lo, Y., Farooqui, A. A., Shui, G., Wenk, M. R., & Ong, W. (2011) · Metabolomics · doi:10.1007/s11306-011-0285-4 · Cited by 8
Covalently bound fatty acids in membrane proteins of some sponges
Ayanoĝlu, E., Rizzolio, M., Beaulieu, S. E., et al. (1990) · Comparative Biochemistry and Physiology Part B Comparative Biochemistry · doi:10.1016/0305-0491(90)90063-y · Cited by 8
Vegetable oils rich in alpha linolenic acid allow a higher accretion of n-3 LCPUFA in the plasma, liver and adipose tissue of the rat
Valenzuela, R., Barrera, C. R., Ayala, J., Julio Sanhueza, C., & Valenzuela, A. (2014) · Grasas y Aceites · doi:10.3989/gya.110113 · Cited by 8
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Ejaz, M., Shikha, F., & Hossain, M. (2013) · Progressive Agriculture · doi:10.3329/pa.v20i1-2.16867 · Cited by 8
Adaptation of Rat Type II Pneumocytes to NO2: Effects of NO2 Application Mode on Phosphatidylcholine Metabolism
Müller, B., Seifart, C., von Wichert, P., & Barth, P. J. (1998) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/ajrcmb.18.5.3097 · Cited by 8
Metabolism of Exogenous Palmitic and Oleic Acids by Preimplantation Mouse Embryos.
Wang, G., & Tsujii, H. (1999) · Journal of Mammalian Ova Research · doi:10.1274/jmor.16.10 · Cited by 8
Organically produced coffee exerts protective effects against the micronuclei induction by mutagens in mouse gut and bone marrow
Silva, J. P., Ferreira, E. B., Barbisan, L. F., Brigagão, M. R. P. L., Paula, F. B. D. A., Moraes, G. O. I., de Magalhães, C. S., & Azevedo, L. C. (2013) · Food Research International · doi:10.1016/j.foodres.2013.07.006 · Cited by 8
Enzymes of phospholipid metabolism in rat pancreatic islets: Subcellular distribution and the effect of glucose and calcium
Rana, R. S., Kowluru, A., & MacDonald, M. J. (1986) · Journal of Cellular Biochemistry · doi:10.1002/jcb.240320206 · Cited by 8

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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