Comparative Analysis of Two Candida parapsilosis Isolates Originating from the Same Patient Harbouring the Y132F and R398I Mutations in the ERG11 Gene
Open accessAbstract
This work presents a comparative analysis of two clinical isolates of C. parapsilosis, isolated from haemoculture (HC) and central venous catheter (CVC). Both strains harboured Y132F and R398I mutations in the gene ERG11 associated with resistance to fluconazole (FLC). Differences between the HC and CVC isolates were addressed in terms of virulence, resistance to FLC, and lipid distribution. Expression of the ERG6 and ERG9 genes, lipid analysis, fatty acid composition, and lipase activity were assessed via qPCR, thin-layer chromatography/high-performance liquid chromatography, gas chromatography, and spectrophotometry, respectively. Regulation of the ERG6 and ERG9 genes did not prove any impact on FLC resistance. Analysis of lipid metabolism showed a higher accumulation of lanosterol in both the isolates regardless of FLC presence. Additionally, a decreased level of triacylglycerols (TAG) with an impact on the composition of total fatty acids (FA) was observed for both isolates. The direct impact of the ERG11 mutations on lipid/FA analysis has not been confirmed. The higher lipase activity observed for C. parapsilosis HC isolate could be correlated with the significantly decreased level of TAG. The very close relatedness between both the isolates suggests that one isolate was derived from another after the initial infection of the host.
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- Authors
- Štefánek, M., Garaiová, M., Valček, A., Jordao, L., & Bujdáková, H.
- Affiliations
- Comenius University Bratislava (SK)
- Slovak Academy of Sciences (SK)
- Research and Development Unit, Department of Environmental Health, National Institute of Health Dr. Ricardo Jorge, 1649-016 Lisboa, Portugal
- Corresponding author
- Bujdáková, Helena
- Venue
- Cells
- Publisher
- MDPI AG
- Published
- 2023-06-07
- Volume
- 12
- Issue
- 12
- Pages
- 1579
- ISSN
- 2073-4409 (ISSN-L)
- PMC
- PMC10296954
- OpenAlex
- W4380048144
- Cited by
- 7
- References
- 61
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 1
- FWCI
- 1.45
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cells (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
- VUBIR (Vrije Universiteit Brussel) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Slovak Research and Development Agency (SK-PT-18-0006, APVV-21-0302, VEGA 2/0036/22)
- Ministry of Education, Science, Research, and the Sport of the Slovak Republic (SK-PT-18-0006, APVV-21-0302, VEGA 2/0036/22)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Keywords
- Candida parapsilosis, ERG11 mutations, fluconazole resistance, ERG6, fungal virulence, lipid metabolism, lanosterol, triglycerides, fatty acid composition, lipase activity, central venous catheters, clinical isolates, strain relatedness
- MeSH terms
- Candida parapsilosis, Antifungal Agents, Humans, Lipase, Lipids, Microbial Sensitivity Tests, Mutation, Fluconazole, Drug Resistance, Fungal
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