Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Pizzo, J. S., da Cruz, V. H. M., Manin, L. P., et al. (2022) · Journal of Mass Spectrometry · doi:10.1002/jms.4900 · Cited by 5
Performance and Tolerance of a Protocol for Idiopathic Chronic Greasy Seborrhea in 18 Dogs Using a Shampoo and Mousse Containing Plant Extracts
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“Total lipids were extracted using the method of Bligh and Dyer [29].”
Method
Potential of siRNA-Bearing Subtilosomes in the Treatment of Diethylnitrosamine-Induced Hepatocellular Carcinoma
Jamal, F., Ahmed, G., Farazuddin, M., Altaf, I., Farheen, S., Zia, Q., Azhar, A., Ahmad, H., Khan, A. A., Somavarapu, S., et al. (2023) · Molecules · doi:10.3390/molecules28052191 · Cited by 5
“Phospholipids were isolated using the method of Bligh and Dyer [60], modified in our laboratory.”
Method
Exposure to Trans Fat During the Developmental Period of Drosophila melanogaster Alters the Composition of Fatty Acids in the Head and Induces Depression-like Behavior
Meichtry, L. B., da Silva, G. S., Londero, L., Dahleh, M. M. M., Bortolotto, V. C., Araújo, S. M., Musachio, E. A. S., da Silva, D. T., Emanuelli, T., Carriço, M. R. S., et al. (2023) · Neuroscience · doi:10.1016/j.neuroscience.2023.03.015 · Cited by 5
New report of Halamphora subtropica (Bacillariophyta) from the Strait of Malacca and its growth and biochemical characterisation under nutrient deprivation
Vello, V., Phang, S., Poong, S., Lim, Y., Ng, F.-L., Shanmugam, J., & Gopal, M. (2023) · Regional Studies in Marine Science · doi:10.1016/j.rsma.2023.102947 · Cited by 6
An Alternative Exploitation of Synechocystis sp. PCC6803: A Cascade Approach for the Recovery of High Added-Value Products
Imbimbo, P., D’Elia, L., Corrado, I., et al. (2023) · Molecules · doi:10.3390/molecules28073144 · Cited by 5
“In particular, lipidic fraction was obtained from the residual biomass by a conve tional method previ usly described [27].”
MethodInfluential
Production and marketing of Tuber floridanum – ecology and gastronomic value of a recently described truffle species
Freiberg, J. A., Grebenc, T., Strojnik, L., et al. (2023) · Scientia Agricola · doi:10.1590/1678-992x-2022-0102 · Cited by 5
Development of a Biosimilar of Agalsidase Beta for the Treatment of Fabry Disease: Preclinical Evaluation
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“[17] and online resource (Supplementary Fig.”
Method
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Jayaraj, J. J. (2023) · 3 Biotech · doi:10.1007/s13205-023-03611-1 · Cited by 5
“The lipid content was determined based on the procedure described by Bligh and Dyer (1959).”
Method
Altered endocannabinoidome bioactive lipid levels accompany reduced DNBS-induced colonic inflammation in germ-free mice
Venneri, T., Giorgini, G., Leblanc, N., Flamand, N., Borrelli, F., Silvestri, C., & Marzo, V. D. (2023) · Lipids in Health and Disease · doi:10.1186/s12944-023-01823-1 · Cited by 5
“HPLC‐MS/MS analysis for quantification of lipid mediators Lipid (namely NAEs, 2-MAGs and 15-lipoxygenase NAE/2-MAG derivatives) extraction was performed following the Bligh and Dyer method [35].”
Method
Progesterone Has No Impact on the Beneficial Effects of Estradiol Treatment in High-Fat-Fed Ovariectomized Mice
Talarico, C. H. Z., Alves, E. S., Santos, J. D. M. D., SUCUPIRA, F., Araujo, L. C. C., & Camporez, J. P. (2023) · Current Issues in Molecular Biology · doi:10.3390/cimb45050253 · Cited by 6
“Tissue TAGs were extracted using the method of Bligh and Dyer [27] and measured using a TAG reagent (Bioclin, Brazil).”
Method
SYNERGISTIC EFFECTS OF NITROGEN DEPRIVATION AND HIGH IRRADIANCE TO ENHANCE BIOMASS AND LIPID PRODUCTION IN NANNOCHLOROPSIS
Mishra, N., Mishra, P., Gupta, E., & Singh, P. (2023) · Journal of Microbiology Biotechnology and Food Sciences · doi:10.55251/jmbfs.3632 · Cited by 7
Investigating the effects of long-term Aroclor 1260 exposure on fatty liver disease in a diet-induced obesity mouse model
Head, K. Z., Bolatimi, O. E., Gripshover, T. C., Tan, M., Li, Y., Audam, T. N., Jones, S. P., Klinge, C. M., Cave, M. C., & Wahlang, B. (2023) · Frontiers in Gastroenterology · doi:10.3389/fgstr.2023.1180712 · Cited by 6
“Hepatic lipids were extracted using a modified Bligh and Dyer method with a 2:1 ratio of chloroform to methanol mixture (27, 28).”
Method
Comparison of indoor (refrigerated) versus outdoor winter storage of commercial honey bee, Apis mellifera (Hymenoptera: Apidae), colonies in the Western United States
Hopkins, B. K., Long, J., Naeger, N. L., & Sheppard, W. S. (2023) · Journal of Economic Entomology · doi:10.1093/jee/toad109 · Cited by 5
Anandamide reduces the migration of lymphocytes to the intestine by CB2 activation and reduces TNF-α in the target organs, protecting mice from graft-versus-host disease
Berg, B. B., Linhares, A. F. S., Martins, D. M., Rachid, M. A., Cáu, S. B. D. A., Galdino, G. S., de Carvalho, J. C. S., Sorgi, C. A., Romero, T. R. L., Pinho, V., et al. (2023) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2023.175932 · Cited by 5
Nocardia australiensis sp. nov. and Nocardia spumae sp. nov., isolated from sea foam in Queensland, Australia
Wright, L., Nouioui, I., Mast, Y., Bunk, B., Spröer, C., Neumann‐Schaal, M., Wolf, J., Katouli, M., & Kurtböke, D. İ. (2023) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.005952 · Cited by 5
Nutritional and Metabolomic Changes of Juvenile Farmed Abalone (Haliotis iris) in New Zealand
Bullon, N., Seyfoddin, A., Dezfooli, S. M., et al. (2023) · Aquaculture Research · doi:10.1155/2023/3297576 · Cited by 5
“A lipid extraction method was modified for small samples [38].”
Method
Lipopolysaccharides from Ralstonia solanacearum induce a broad metabolomic response in Solanum lycopersicum
Zeiss, D. R., Molinaro, A., Steenkamp, P. A., Silipo, A., Piater, L. A., Lorenzo, F. D., & Dubery, I. A. (2023) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2023.1232233 · Cited by 6
Evaluation of a Saccharomyces cerevisiae Fermentation Product on the Feedlot Performance and Carcass Merit of Hair Lambs Offered an Annual Ryegrass-Hay-Based Finishing Diet: A Pilot Study
Burt, J. C., Boyd, J. A., Baxter, L. L., Garcia-Galicia, I. A., & Kerley, B. P. (2023) · Animals · doi:10.3390/ani13162630 · Cited by 6
Accumulation of oxysterols in the erythrocytes of COVID-19 patients as a biomarker for case severity
Khedr, A., Khayat, M. T. A., Khayyat, A. N., et al. (2023) · Respiratory Research · doi:10.1186/s12931-023-02515-1 · Cited by 5
Formation of RRR-α-tocopherol in rumen and intestinal digestibility of tocopherols in dairy cows
Lashkari, S., Panah, F. M., Weisbjerg, M. R., et al. (2023) · Animal nutrition · doi:10.1016/j.aninu.2023.07.007 · Cited by 5
The MprF homolog LysX synthesizes lysyl-diacylglycerol contributing to antibiotic resistance and virulence
Gill, C. P., Phan, C., Platt, V., et al. (2023) · Microbiology Spectrum · doi:10.1128/spectrum.01429-23 · Cited by 5
“Lipids were prepared using the Bligh-Dyer procedure (77), with modifications as described (78).”
Effects of Quercetin Nanoemulsion on Cholesterol Efflux and MicroRNA-33/34a Expression in the Liver of Mice Fed with a High-Cholesterol Diet
Lee, M.-S., & Kim, Y. (2023) · Preventive Nutrition and Food Science · doi:10.3746/pnf.2023.28.3.271 · Cited by 5
“Total lipids were extracted from the liver tissue (0.5 g) using a mixed solvent of chloroform-methanol (2:1, v/v) according to the modified method of Bligh and Dyer (1959).”
MethodInfluential
Oral Squamous Cell Carcinoma Lipid Evaluation in Gingiva Tissue Stored in TRIzol via Shotgun Lipidomics and MALDI Mass Spectrometry Imaging
Bernardo, R. A., Júnior, C. I. D. O., Roque, J. V., et al. (2023) · Journal of Proteome Research · doi:10.1021/acs.jproteome.3c00216 · Cited by 5
Evaluating methods for determining mercury concentrations in ancient marine fish and mammal bones as an approach to assessing millennial-scale fluctuations in marine ecosystems
Avery, J. P., Castellini, J. M., Misarti, N., et al. (2023) · Frontiers in Ecology and Evolution · doi:10.3389/fevo.2023.1251282 · Cited by 5
Effect of digestible protein on intermediate metabolism, hepatic enzyme activities, energy reserves, and growth performance of pacu (Piaractus mesopotamicus) in the finishing growth phase
Oshiro, A. M., Khan, K. U., Carli, G. C., et al. (2023) · Fish Physiology and Biochemistry · doi:10.1007/s10695-023-01243-z · Cited by 5
“Hepatic total lipid content was determined using the solvent extraction method described by Bligh and Dyer (1959).”

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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