Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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MacDonald, G. E., Lada, R. R., Caldwell, C. D., et al. (2019) · Trees · doi:10.1007/s00468-019-01918-2 · Cited by 5
Biochemical indicators of green photosynthetic bacteria Chlorobium limicola response to Cu(2+) action
Segin, T., Hnatush, S. О., Maslovska, O., Halushka, A. A., & Zaritska, Y. (2020) · The Ukrainian Biochemical Journal · doi:10.15407/ubj92.01.103 · Cited by 5
“Bligh method [15], after which the mixture of chloroform with methanol was dried on water bath and methylation of obtained fatty acids using 2 M sodium methylate was performed.”
Method
Quantification of diacylglycerol and triacylglycerol species in human fecal samples by flow injection Fourier transform mass spectrometry
Ertl, V. M., Höring, M., Schött, H.-F., Blücher, C., Kjølbæk, L., Astrup, A. V., Burkhardt, R., & Liebisch, G. (2020) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-020-02416-y · Cited by 5
Fraud with the addition of cow's milk alters the lipid fraction of buffalo mozzarella
Pignata-Viana, M. C., Fernandes, S. A. D. A., da Silva, A. G., et al. (2020) · Food Science and Technology · doi:10.1590/fst.19619 · Cited by 5
“Total lipid extraction Lipids were extracted using a chloroform/methanol/water solution, according to Bligh & Dyer (1959).”
Method
Involvement of lipids from Leishmania braziliensis promastigotes and amastigotes in macrophage activation
Carfagna, I. E., Penas, F., Bott, E., et al. (2020) · Molecular Immunology · doi:10.1016/j.molimm.2020.06.023 · Cited by 5
Desenvolvimento e aceitabilidade de bolo de chocolate acrescido de farinha de maná-cubiu (Solanum sessiliflorum Dunal)
Eggea, V., Medeiros, C. O., Queiroz, C., et al. (2019) · Research Society and Development · doi:10.33448/rsd-v9i2.1973 · Cited by 5
Plasma phospholipid dysregulation in patients with cystathionine-β synthase deficiency
Minno, A. D., Anesi, A., Chiesa, M., et al. (2020) · Nutrition Metabolism and Cardiovascular Diseases · doi:10.1016/j.numecd.2020.07.019 · Cited by 5
Test–Retest Reliability and Correlates of Vertebral Bone Marrow Lipid Composition by Lipidomics Among Children With Varying Degrees of Bone Fragility
Whitney, D. G., Devlin, M. J., Alford, A. I., Modlesky, C. M., Peterson, M. D., Li, Y., & Caird, M. S. (2020) · JBMR Plus · doi:10.1002/jbm4.10400 · Cited by 5
“Briefly, for each bone marrow sample, lipids were extracted using a modified Bligh-Dyer method of solvent partition.((35)) An aliquot of 100 μL was taken and 200 μL of water was added.”
Method
Quality Appraisal of Labeo rohita Roe Protein Concentrate and Characterization of the Roe Protein Concentrate-Based Extruded Munchies
Chakraborty, P., Pramanik, A., Bhattacharyya, D. K., et al. (2020) · Journal of Aquatic Food Product Technology · doi:10.1080/10498850.2020.1818017 · Cited by 5
Lipid composition of raw and grilled beef cattle slaughtered at four body weights
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“For the analysis of total lipids, the extraction with chloroform-methanol (1:2, v/v) was used according to the methodology described by Bligh and Dyer (1959).”
Method
A noveln-3 glyceride mixture enhances enrichment of EPA and DHA after single dosing in healthy older adults: results from a double-blind crossover trial
Fisk, H. L., Kindberg, G. M., Hustvedt, S. O., & Calder, P. C. (2020) · British Journal Of Nutrition · doi:10.1017/s0007114520004031 · Cited by 5
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Zhou, Q., Tan, X., Meng, X., et al. (2020) · Biotechnology and Applied Biochemistry · doi:10.1002/bab.2070 · Cited by 5
Effects of salting technology and smoking protocol on yield and quality of hot‐smoked Atlantic salmon ( Salmo salar L.)
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Ahern, G. J., van Sinderen, D., Yang, B. K., Ross, R. P., & Stanton, C. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1274-3_8 · Cited by 5
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Zhang, C., Ren, H., & Jiang, L. (2021) · Arabian Journal of Chemistry · doi:10.1016/j.arabjc.2021.103108 · Cited by 5
Fatty acid profile and chemical composition of meat from Nellore steers finished on pasture with different amounts of supplementation
Silva, R. R., do Prado, I. N., da Silva, F. F., Rotta, P. P., Rodrigues, L. B. O., do Prado, R. M., Mesquita, B. M. A. D. C., Alba, H. D. R., & de Carvalho, G. G. P. (2021) · Canadian Journal of Animal Science · doi:10.1139/cjas-2020-0099 · Cited by 5
Growth enhancement in marine copepod, Pseudodiaptomus annandalei fed with the sodium acetate containing N/P starved medium grown, Tetraselmis suecica
Kumar, S. D., Krishnaveni, N., Santhanam, P., Raju, P., Perumal, P., Begum, A., Ahmed, S. U., Pragnya, M., Dhanalakshmi, B., & Kim, M. (2021) · Aquaculture Research · doi:10.1111/are.15254 · Cited by 5
Shotgun Lipidomic Analysis of Leishmania Cells
Zhang, K., & Hsu, F. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1410-5_14 · Cited by 5
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Castro, V. R., Furtado, M. C. D. S., Bermúdez, V. M. S., et al. (2021) · Research Society and Development · doi:10.33448/rsd-v10i7.16226 · Cited by 5
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Озолина, Н. В., Kapustina, I. S., Гурина, В. В., et al. (2021) · The Journal of Membrane Biology · doi:10.1007/s00232-021-00194-x · Cited by 5

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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