Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Jedidi, H., Champagne, C. P., Raymond, Y., et al. (2014) · International Dairy Journal · doi:10.1016/j.idairyj.2014.02.006 · Cited by 4
Fontes energéticas vegetais para juvenis de jundiá e carpa
Corrêia, V., da Silva, L. P., Pedron, F. A., et al. (2012) · Arquivo Brasileiro de Medicina Veterinária e Zootecnia · doi:10.1590/s0102-09352012000300022 · Cited by 4
Production, composition and processing of milk from ewes fed soybean seeds
Zeola, N. M. B. L., Sobrinho, A. G. D. S., Hatsumura, C. T., et al. (2015) · Revista Brasileira de Zootecnia · doi:10.1590/s1806-92902015000400003 · Cited by 4
Microalgae for Bioenergy: Key Technology Nodes
Maléterová, Y., Kaštánek, F., Rousková, M., Matějková, M., Kaštánek, P., & Šolcová, O. (2015) · The Scientific World JOURNAL · doi:10.1155/2015/597618 · Cited by 4
The benthic association between a bivalve and a shell boring polychaete and their potential food sources
Silina, A. V., & Zhukova, N. V. (2012) · Oceanology · doi:10.1134/s0001437012050153 · Cited by 4
SHELF LIFE AND BIOCHEMICAL COMPOSITION OF BONITO FISH (Sarda sarda, BLOCH, 1758) STORED AT 4C AND FISHING WITH DIFFERENT FISHING TOOLS
Erdem, M. E., Kalaycı, F., Duyar, H. A., et al. (2009) · Journal of Muscle Foods · doi:10.1111/j.1745-4573.2009.00148.x · Cited by 4
Effects of Fat-Enriched Diet on the Content of Sphingolipids in the Brain and on Cognitive Functions in Old Rats
Babenko, N. A., Semenova, Y. A., & Kharchenko, V. S. (2009) · Neurophysiology · doi:10.1007/s11062-010-9101-4 · Cited by 4
Biliary excretion of radioactivity after intravenous administration of [3H]25-hydroxyvitamin D3 in man
Ledger, J. E., Watson, G., & Compston, J. E. (1986) · Digestive Diseases and Sciences · doi:10.1007/bf01311670 · Cited by 3
Strong light elevates thermotolerance of photosynthetic apparatus and the content of membranes and polar lipids in wheat leaves
Kislyuk, I. M., Bubolo, L. S., Bykov, O. D., et al. (2013) · Russian Journal of Plant Physiology · doi:10.1134/s1021443713050099 · Cited by 4
Chemical reagents as probes: Application to fish protein gels and detection of a cysteine TGase in hake
Cardoso, C. L., Mendes, R. O., Vaz‐Pires, P., et al. (2010) · LWT · doi:10.1016/j.lwt.2010.12.015 · Cited by 4
An L‐Form of Staphylococcus aureus Adapted to a Brain Heart Infusion Medium without Osmotic Stabilizers
Takahashi, T., Tadokoro, I., & Adaghi, S. (1981) · Microbiology and Immunology · doi:10.1111/j.1348-0421.1981.tb00092.x · Cited by 4
Isolation of intact proplastids from suspension cultured cells of Theobroma cacao L.: de novo long chain fatty acid biosynthesis
Leathers, R., & Scragg, A. H. (1993) · Plant Science · doi:10.1016/0168-9452(93)90089-i · Cited by 4
Age-Related Changes in the Phospholipase D-Dependent Signal Pathway of Insulin in the Rat Neocortex
Babenko, N. A., & Kharchenko, V. S. (2013) · Neurophysiology · doi:10.1007/s11062-013-9346-9 · Cited by 4
Acyl-chain remodeling of dioctanoyl-phosphatidylcholine in Saccharomyces cerevisiae mutant defective in de novo and salvage phosphatidylcholine synthesis
Kishino, H., Eguchi, H., Takagi, K., et al. (2014) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2014.01.136 · Cited by 4
Farinhas de silagem de resíduo de pescado co-secas com farelo de arroz: uma alternativa viável.
Benites, C. I., & de Souza-Soares, L. A. (2007) · Archivos de Zootecnia · doi:10.21071/az.v59i227.4720 · Cited by 4
Hyaluronidase Alters the Lipid Profile of Cumulus Cells as Detected by MALDI‐TOF MS and Multivariate Analysis
Montani, D. A., Regiani, T., Victorino, A. B., et al. (2014) · Lipids · doi:10.1007/s11745-014-3931-6 · Cited by 4
Incorporation of conjugated linoleic acid (CLA) and α-linolenic acid (LNA) in pacu fillets
Barilli, D. J., Santarosa, M., Zanqui, A. B., et al. (2014) · Food Science and Technology · doi:10.1590/s0101-20612014005000010 · Cited by 4
Desempenho e características de carcaça do escargot francês (Helix aspersa maxima) alimentado com rações contendo diferentes óleos vegetais
Hayashi, C., Soares, C. M., Matsushita, M., et al. (2004) · Ciência Rural · doi:10.1590/s0103-84782004000100036 · Cited by 4
Maternal Baicalin Treatment Increases Fetal Lung Surfactant Phospholipids in Rats
Chen, C., Wang, L.-F., & Cheng, K.-T. (2009) · Evidence-based Complementary and Alternative Medicine · doi:10.1093/ecam/nep073 · Cited by 4
Effect of cheese as a fat replacer in fermented sausage
Ercoşkun, H. (2012) · Journal of Food Science and Technology · doi:10.1007/s13197-012-0670-7 · Cited by 4
Assessing low-dose gentamicin-induced kidney injury in rats by analysis of urine
Nykjær, A., Schambye, H. T., Pedersen, A. H., et al. (2009) · Journal of Pharmacological and Toxicological Methods · doi:10.1016/j.vascn.2009.07.006 · Cited by 4
Cultivation of Microorganisms in the Cultural Medium Made from Squid Internal Organs and Accumulation of Polyunsaturated Fatty Acids in the Cells
Morita, N., Ichise, N., Yumoto, I., et al. (2005) · Biotechnology Letters · doi:10.1007/s10529-005-7187-3 · Cited by 4
Non-selective distribution of isomeric cholesterol hydroperoxides to microdomains in cell membranes and activation of matrix metalloproteinase activity in a model of dermal cells
Nakamura, T., Noma, A., Shimada, S., et al. (2013) · Chemistry and Physics of Lipids · doi:10.1016/j.chemphyslip.2013.05.004 · Cited by 4
Lysophosphatidic Acid Produced by Hen Egg White Lysophospholipase D Induces Vascular Development on Extraembryonic Membranes
Morishige, J., Uto, Y., Hori, H., et al. (2013) · Lipids · doi:10.1007/s11745-013-3765-7 · Cited by 4
Biliary Secretion of Anionic Polypeptide Fraction Is Not Coupled to That of Phospholipids and Cholesterol in Rats
Verkade, H. J., Kuipers, F., Domingo, N., et al. (1997) · Hepatology · doi:10.1002/hep.510250108 · Cited by 4
The impact of environmental perturbation on microbial community structure and dynamics: Factors affecting growth ofClonothrix fuscain groundwater
Vigliottá, G., Talà, A., Giudetti, A. M., et al. (2007) · Journal of Plant Interactions · doi:10.1080/17429140701578339 · Cited by 4
Incorporation and fatty acid composition in liver of Nile tilapia fed with flaxseed oil
Visentainer, J. V., França, P. B., de Aguiar, A. C., et al. (2011) · Acta Scientiarum. Technology/Acta scientiarum. Technology · doi:10.4025/actascitechnol.v33i2.7970 · Cited by 4
A simple and sensitive determination for plasmalogen lysophosphatidylethanolamine in rabbit platelets
Morikawa, S., Taniguchí, S., Fujiwara, M., et al. (1989) · Thrombosis Research · doi:10.1016/0049-3848(89)90051-0 · Cited by 4
Utilização de alho e cobre na alimentação de frangos de corte
Togashi, C. K., Fonseca, J. B., Soares, R. D. T. R. N., et al. (2008) · Revista Brasileira de Zootecnia · doi:10.1590/s1516-35982008000600013 · Cited by 4

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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