Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Method
Mass spectrometry-based lipidomic analysis reveals altered lipid profile in brain tissues resected from patients with focal cortical dysplasia (FCD)
Kumar, K., Yadav, N., Banerjee, J., et al. (2021) · Epilepsy Research · doi:10.1016/j.eplepsyres.2021.106773 · Cited by 4
Functional Properties of Yellowfin Tuna (Thunnus albacares) Skin Collagen Hydrolysate Fraction obtained by Ultrafiltration Purification
Nguyen, B. C., Nguyen, H. M. X., Nguyen, K. H. N., & Kha, T. C. (2021) · Current Research in Nutrition and Food Science Journal · doi:10.12944/crnfsj.9.3.12 · Cited by 5
Microbial biodiesel: a comprehensive study toward sustainable biofuel production
Pradhan, S., Malani, R. S., & Mishra, A. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-822810-4.00018-x · Cited by 4
TEST RESULTS OF STARTING COMPOUND FEEDS FOR INDUSTRIAL CULTIVATION OF SALMONIDS (CHUM SALMON)
Bashtovoy, A., Николаевна, Т. Г., Yarochkin, A., et al. (2021) · Fisheries · doi:10.37663/0131-6184-2021-6-91-96 · Cited by 4
Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics
Blasco, J., Vélez, E. J., Perelló-Amorós, M., Azizi, S., Capilla, E., Fernández‐Borràs, J., & Gutiérrez, J. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms222313107 · Cited by 4
“The latter was determined gravimetrically after drying the samples at 95 ◦C for 24 h. Lipids were extracted as described by Bligh and Dyer [62]; lipid extracts were dried under a N2 atmosphere, and total lipids determined gravimetrically.”
Method
The decreased PG content of pgp1 inhibits PSI photochemistry and limits reaction center and light-harvesting polypeptide accumulation in response to cold acclimation
Ivanov, A. G., Król, M., Savitch, L. V., et al. (2022) · Planta · doi:10.1007/s00425-022-03819-0 · Cited by 4
Use of blend based on an emulsifier, monolaurin, and glycerides of butyric acid in the diet of broilers: impacts on intestinal health, performance, and meat
Almeida, A. B., Araújo, D. N., Strapazzon, J. V., et al. (2021) · Anais da Academia Brasileira de Ciências · doi:10.1590/0001-3765202120210687 · Cited by 4
Deuterium‐depleted water: A new tracer to label pulmonary surfactant lipids in adult rabbits
Simonato, M., Ricci, F., Catozzi, C., et al. (2022) · Journal of Mass Spectrometry · doi:10.1002/jms.4808 · Cited by 4
“Lipids were extracted by the method of Bligh and Dyer.(13) Extracted lipids were oxidized with osmium tetroxide, resuspended in chloroform and spotted on silica gel G thin-layer plates (Merck, Darmstadt, Germany).”
Method
Assessment of Moringa oleifera Lam. Seeds Potential as an Adsorbent Material for Soybean Oil Bleaching
de Souza, P. M., Galuch, M. B., Lopes, A. P., Pizzo, J. S., Castro, M. C., da Cruz, V. H. M., Souza, P. R., Impossetto, D. F., Suzuki, R. M., & Visentainer, J. V. (2022) · Revista Virtual de Química · doi:10.21577/1984-6835.20220006 · Cited by 4
Extraction and Analysis of Lipids
Wanasundara, P. K. J. P. D., & Shahidi, F. (2008) · Food science and technology · doi:10.1201/9781420046649.ch5 · Cited by 4
Extracellular superoxide dismutase (EC-SOD) R213G variant reduces mitochondrial ROS and preserves mitochondrial function in bleomycin-induced lung injury
Elajaili, H. B., Hernandez‐Lagunas, L., Harris, P. S., Sparagna, G. C., Jonscher, R. L., Ohlstrom, D. J., Sucharov, C. C., Bowler, R. P., Suliman, H. B., Fritz, K. S., et al. (2022) · Advances in Redox Research · doi:10.1016/j.arres.2022.100035 · Cited by 4
A modified material‐saving method system for detecting and analysing fatty acids in fish using gas chromatography
Zhang, J., Ren, Y., Sui, X., et al. (2022) · Aquaculture Research · doi:10.1111/are.15832 · Cited by 4
Chemical characterization and potential use of reptile fat from sustainable programs
Leiva, P. M. L., Valli, F. E., Piña, C. I., González, M. A., & Simoncini, M. S. (2022) · Ethnobiology and Conservation · doi:10.15451/ec2022-03-11.06-1-12 · Cited by 5
Characterization of Ceramides and Glucosylceramides of the Satsuma Mandarin(Citrus unshiu) Fruit
Mukai, K., Takeuchi, M., Ohnishi, M., et al. (2022) · Journal of Oleo Science · doi:10.5650/jos.ess21376 · Cited by 4
Kupffer cells are protective in alcoholic steatosis
Köhler, N., Höring, M., Czepukojc, B., Rose, T. D., Buechler, C., Kröhler, T., Haybaeck, J., Liebisch, G., Pauling, J. K., Kessler, S. M., et al. (2022) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2022.166398 · Cited by 4
Hydrogenophaga crocea sp. nov. associated with cyanobacterial mat isolated from farmland mud
Thorat, V., Tiwarekar, B., Kirdat, K., et al. (2022) · Archives of Microbiology · doi:10.1007/s00203-022-02865-2 · Cited by 4
“Methanol/ chloroform/0.3% sodium chloride (2:1:0.8, by vol.) were used for extraction of polar lipids (Bligh and Dyer 1959) in addition to the modifications of Card (1973).”
MethodInfluential
Age-Dependent Increase in Schmidt-Lanterman Incisures and a Cadm4-Associated Membrane Skeletal Complex in Fatty Acid 2-hydroxylase Deficient Mice: a Mouse Model of Spastic Paraplegia SPG35
Jordans, S., Hardt, R., Becker, I., et al. (2022) · Molecular Neurobiology · doi:10.1007/s12035-022-02832-4 · Cited by 4
“Total lipid extracts from sciatic nerves were prepared as described [20].”
Method
The effect of major nutrients in five levels of an f medium on growth and proximal composition of Thalassiosira weissflogii
Peraza-Yee, M. M., Carranza-Díaz, O., Bermudes-Lizárraga, J. F., Peraza, D. J. L., Nieves-Soto, M., & Millán-Almaraz, M. I. (2022) · Latin American Journal of Aquatic Research · doi:10.3856/vol50-issue1-fulltext-2749 · Cited by 4
“(1956) at 485 nm; while lipids were extracted as described by Blight & Dyer (1959) and determined according to Pande et al.”
MethodInfluential
Halorubrum salipaludis sp. nov., isolated from the saline–alkaline soil
Gong, Q., Zhao, P., Miao, S., et al. (2021) · Archives of Microbiology · doi:10.1007/s00203-021-02729-1 · Cited by 4
Analysis of Content of 2-Oxoacids in Rat Brain Extracts Using High-Performance Liquid Chromatography
Tashlitsky, V. N., Artiukhov, A. V., Fedorova, N. V., et al. (2022) · Biochemistry (Moscow) · doi:10.1134/s0006297922040058 · Cited by 4
Mechanisms and Implications of Bacterial Invasion across the Human Skin Barrier
Lipsky, Z. W., Patsy, M., Marques, C. N. H., & German, G. K. (2022) · Microbiology Spectrum · doi:10.1128/spectrum.02744-21 · Cited by 4
“Louis, MO) for 60 min, partially depleting intercellular ceramide, cholesterol, and free fatty acid lipids found in SC from the tissue (11, 17, 33, 55, 80).”
Background
An Efficient Method for Extraction and Enrichment of γ-Linolenic Acid (GLA) from Spirulina
(2021) · Letters in Applied NanoBioScience · doi:10.33263/briac111.31663174 · Cited by 5
Breast milk fatty acid composition of women living far from the coastal area in Brazil
Nishimura, R., de Castro, G. S., Jordão, A. A., et al. (2013) · Jornal de Pediatria (Versão em Português) · doi:10.1016/j.jpedp.2012.11.005 · Cited by 4
Influence of sexual stimulation on sperm parameters in semen samples collected via masturbation from normozoospermic men or cryptozoospermic men participating in an assisted reproduction programme
Yamamoto, Y., Sofikitis, N., Mio, Y., et al. (2009) · Andrologia · doi:10.1111/j.1439-0272.2000.tb02877.x · Cited by 4
Biofixation of Air Emissions and Biomass Valorization—Evaluation of Microalgal Biotechnology
Biscaia, W. L., Miyawaki, B., de Mello, T. C., de Vasconcelos, E. C., de Arruda, N. M. B., & Maranho, L. T. (2022) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-022-03972-y · Cited by 5
Phenylobacterium glaciei sp. nov., isolated from Vestrebroggerbreen, a valley glacier in Svalbard, Arctic
Thomas, F. A., Sinha, R. K., Hatha, A. A. M., et al. (2022) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.005375 · Cited by 4
Seasonal variation in the growth, lipid accumulation, and fatty acid composition of Chlorella sp. GN1 cultured in flat plate photobioreactors outdoors
Feng, P., Qin, L., Xu, Z., Alam, M. A., Wang, Z., & Zhu, S. (2022) · Biomass Conversion and Biorefinery · doi:10.1007/s13399-022-02810-9 · Cited by 4
Effect of three-component antioxidant blend on oxidative stability and nitrite reduction of cooked sausages
Kolev, N. I., Vlahova‐Vangelova, D., Balev, D. K., et al. (2022) · Acta Scientiarum Polonorum Technologia Alimentaria · doi:10.17306/j.afs.1052 · Cited by 4
Effects of Taurine on Primary Metabolism and Transcription in a Coral Symbiodinium sp.
Huang, A., Shi, H., Cui, R., et al. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.797688 · Cited by 4
Functional Characterization of the Monogalactosyldiacylglycerol Synthase Gene ptMGD2 in the Diatom Phaeodactylum tricornutum
Shang, S., Liu, R., Luo, L., Li, X., Zhang, S., Zhang, Y., Zheng, P., Chen, Z., & Wang, B. (2022) · Frontiers in Marine Science · doi:10.3389/fmars.2022.874448 · Cited by 4
Evaluation of maturation promoting factor in polychaete ( Marphysa sp.) on Indian White Prawn , Penaeus indicus female broodstock
Santander‐Avanceña, S. S., Traifalgar, R. F. M., Monteclaro, H. M., et al. (2022) · Aquaculture Research · doi:10.1111/are.16003 · Cited by 4

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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