Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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“To remove lipids, the samples underwent solvent extraction using a 2:1 mixture of dichloromethane and methanol (v/v) with sonication, based on a Bligh and Dyer (1959) protocol with modifications.”
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“For fatty acid methyl ester (FAME) analysis, Lipids were extracted from 300 mg of biomass pretreated with HCl 2 mol/L and then subjected to the Bligh and Dyer protocol [26, 27].”
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Ali, H., Yamanishi, M., Tsuchiya, M., Hasi, R. Y., Matsusaki, M., Saio, T., Aihara, M., Kawakami, R., & Tanaka, T. (2025) · Lipids · doi:10.1002/lipd.70005
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Selvi, M. J. T., Rani, V., Padmavathy, P., Uma, A., Shalini, R., Sivaraman, B., Subash, P., & Kadam, R. V. (2025) · Thalassas An International Journal of Marine Sciences · doi:10.1007/s41208-025-00929-w
“Pooled extracts were dried at 80 °C for 24 h, and lipid content was calculated (Bligh and Dyer 1959; Gupta et al. 2019).”
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Ye, J. S., Purlyte, E., López, V. A., Mill, J., Tatge, L., Kizzar, T., Baldwin, D. A., Rashan, E. H., Kim, J., Pagliarini, D. J., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.09.10.675409
Mechanisms of 10-hydroxyoctadecanoic acid resistance in Streptococcus pneumoniae
Johnson, C. N., Frank, M. W., Chaguza, C., Morrow, B. T., Jia, Q., Radka, C. D., & Rosch, J. W. (2025) · Journal of Bacteriology · doi:10.1128/jb.00223-25
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“Total lipid concentration was assessed through gravi-metric method, following a modified version of Bligh and Dyer (1959) method.”
Effect of heat stress on metabolic analysis of sweat in dairy cattle
Anitaş, Ö., Göncü, S., Hepsağ, F., & Özoğul, Y. (2025) · Revista de Ciências Agroveterinárias · doi:10.5965/223811712422025355
“The method used by BLIGH & DYER (1959) Relative humidity (”
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“Lipid extraction was performed by the gravimetric method based on Bligh & Dyer (1959) with modifications.”
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Rajasekaran, B., Pan, M., Buamard, N., et al. (2025) · Journal of Food Science · doi:10.1111/1750-3841.70598
“Before analysis, oil from the fish mince was extracted using the method of Bligh and Dyer (1959).”
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“Total lipids were extracted using the Bligh and Dyer method [16].”
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“Fully expanded, healthy leaves were selected and dark-adapted for 30 min using leaf clips prior expressed as percentages of total fatty acids (% of total FA composition) (Bligh and Dyer 1959).”
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“%) solution as described 40 .”
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“Lipids were then extracted by the Bligh and Dyer method 25 and samples were resuspended in isopropanol.”
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Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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