Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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da Cruz, V. H. M., Pizzo, J. S., Santos, P. D. S., et al. (2023) · Food Chemistry Advances · doi:10.1016/j.focha.2023.100508
Boosting biomass, lipid content, carbon sequestration capacity of microalgae cultivated with aggregation-induced emission light-converting filmsPreprint
Song, Y., Xie, L., Zhang, X., Hu, Z., Li, S., Zhang, P., & Yang, X. (2023) · Research Square · doi:10.21203/rs.3.rs-3510304/v1
Elaboração de pães de fôrma com adição de farinha de ora-pro-nóbis
Simão, Y. O. A. D., Ribeiro, P. F. D. A., & Kaminski, T. A. (2023) · Research Society and Development · doi:10.33448/rsd-v12i11.43643
Towards Understanding Fate of Lipids in Anaerobic Digestion: Gc-Fid Method for Lipid Hydrolysis ObservationPreprint
Šánek, L., & Pěcha, J. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4625496
Variations in lipid profile of of the marine microalga Nannochloropsis salina in four different culture media
Ganga, R. S., Anandan, R., & Joseph, A. (2022) · Indian Journal of Fisheries · doi:10.21077/ijf.2022.69.3.104044-10 · Cited by 1
“Total lipids were extracted as per Bligh and Dyer (1959).”
Method
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Upadhyay, A. K., Singh, D. V., Singh, R. P., Mandotra, S. K., Singh, L., Singh, N. K., Pandey, S., Saxena, G., & Arora, N. K. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4620072
Thu nhận và tính chất của collagen dạng vảy và dịch collagen thủy phân dịch thủy phân collagen từ vảy cá chẽm (Lates calcarifer)
Phan, T. P. D., Trung, T. S., & Nguyễn, V. H. (2023) · Tạp chí Khoa học - Công nghệ Thủy Sản Trường Đại học Nha Trang · doi:10.53818/jfst.03.2023.199
“Hàm lượng lipid được xác định theo phương pháp đã công bố trong nghiên cứu trước đây [16]”
Multivariate analysis of agronomic, physicochemical, and physiological characters of passion fruit hybrids cultivated at different environments
Maciel, K. S., de Lima, P. A. M., Mengarda, L. H. G., et al. (2023) · Brazilian Journal of Biology · doi:10.1590/1519-6984.275338
Synchronous Enhancement of Astaxanthin and Lipid Accumulation in Haematococcus Pluvialis Through Co-Mutation of Ethanol and Artp: An Exploration of Characteristics and Underlying MechanismsPreprint
Yihua, M., Sun, X., Sun, Y., et al. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4632160
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Lu, H.-C., Melvin, S. D., Ziajahromi, S., et al. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4632060
"Potensi Asam Eikosapentanoat (EPA) Pada Ganggang Merah (Gracilaria sp.) sebagai Suplementasi Nutrisi untuk Mengurangi Risiko Penyakit Kardiovaskular"
Putri, L. A. L. N. (2023) · Prosiding Workshop dan Seminar Nasional Farmasi · doi:10.24843/wsnf.2022.v02.p10
“Lipid diekstraksi menggunakan kloroform dan metanol (1:2, v/v) menggunakan metode Bligh and Dyer (1959) [15].”
Background
Effect of nanoscale zero valent iron toward bacteria and their response
Kotchaplai, P. (2016) · Chula Digiverse · doi:10.58837/chula.the.2016.1567
Perfil sensorial dinâmico e direcionadores de preferência em bebida funcional de baixa caloria à base de tamarindo - Tamarindus indica L
Lima, R. S. (2020) · doi:10.47749/t/unicamp.2020.1161064
Produção de partículas com óleo essencial de laranja e óleo de peixe por vacuum spray drying e spray drying
Ramos, F. D. M. (2020) · doi:10.47749/t/unicamp.2020.1166467
Efeito dos sistemas de cultivo e limitações de nutriente para o crescimento e biossíntese de metabólitos nas microalgas "Botryococcus terribilis" e Chlorella sp
Estevam, B. R. (2021) · doi:10.47749/t/unicamp.2021.1238132
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Das, L. M., Kujur, R. R. A., Debnath, T., et al. (2023) · Folia Microbiologica · doi:10.1007/s12223-023-01111-6
“Polar lipids were extracted from 200-mg dry cells described by Bligh and Dyer (1959) and separated by two-dimensional TLC (silica gel 60 F254, cat no. 1.05554.0007; Merck).”
Influential
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“Chromosorb W/DP with a Silar 5CP phase (Serva, Germany) was used as a carrier, the concentration of which was 10 %, at a temperature from 140 to 250 ° C and a ramp rate of 2 ° C/min (injector temperature – 210 ° C; temperature detector – 240 ° C) [15, 16].”
Method
Exploring the activity and the essentiality of the putative Δ6-desaturase in the procyclic and bloodstream forms of Trypanosoma bruceiPreprint
Cerone, M., & Smith, T. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.11.23.568515
Relative Body Size Relationship to Proximate Composition of Farmed Hypophthalmichthys nobilis from Bahawalnagar, Pakistan
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Go, Y. W., & Yeom, S. H. (2023) · KSBB Journal · doi:10.7841/ksbbj.2023.38.3.194
Optimization of the Traditional Process of Oil Extraction from Sardine Viscera (Sardina pilchardus) in the Far North Region-Cameroon by the Response Surface Methodology
Chimi, B. J., Ghomdim, H. N., Ngangoum, E. S., et al. (2023) · Asian Journal of Biotechnology and Bioresource Technology · doi:10.9734/ajb2t/2023/v9i4189
Aceitação de carne ovina no Brasil: uma abordagem por ferramentas de marketing, técnicas projetivas e análises sensoriais
Battagin, H. V. (2023) · doi:10.11606/t.74.2023.tde-05122023-103245
The Cultivation of Tea: From the Nursery to the Fields
Chatterjee, A. K. (2023) · International Journal of Chemical and Environmental Sciences · doi:10.15864/ijcaes.5105
Characterization of PhaP Protein from Bacillus thuringiensis serovar indiana using software tools
Basak, A. (2023) · International Journal of Chemical and Environmental Sciences · doi:10.15864/ijcaes.5102
Chemically Driven Water Oxidation Catalyzed by Ruthenium Complexes: An Overview
Kundu, A. (2023) · International Journal of Chemical and Environmental Sciences · doi:10.15864/ijcaes.5103
Synthesis, Characterisation and Microbial Activity of 2-(1H-benzothiazol-2-yl) Thioacetic acid with some Bivalent Metal Ions
Ali, I., Farooq, F., Wara, M. K., et al. (2023) · International Journal of Chemical and Environmental Sciences · doi:10.15864/ijcaes.5101
Microbial metabolic specificity controls pelagic lipid export efficiencyPreprint
Behrendt, L., Alcolombri, U., Hunter, J. E., et al. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.12.08.570822
“Bulk lipids were extracted from frozen, pelleted cells of Phaeodactylum tricornutum via a Bligh & Dyer extraction protocol 40 .”
Method
Targeting Fatty Acid Amide Hydrolase Counteracts the Epithelial-to-Mesenchymal Transition in Keratinocyte-Derived Tumors
Kovacs, D., Flori, E., Bastonini, E., Mosca, S., Migliano, E., Cota, C., Zaccarini, M., Briganti, S., & Cardinali, G. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms242417379

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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