Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
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Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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The Assessment of Phytoremediation Potential of Invasive Weed Amaranthus spinosus L.
Chinmayee, M. D., Mahesh, B., Pradesh, S., et al. (2012) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-012-9657-0 · Cited by 83
Supplementation with antioxidant-rich extra virgin olive oil prevents hepatic oxidative stress and reduction of desaturation capacity in mice fed a high-fat diet: Effects on fatty acid composition in liver and extrahepatic tissues
Rincón‐Cervera, M. Á., Valenzuela, R., Hernández‐Rodas, M. C., Marambio, M., Espinosa, A., Mayer, S., Romero, N., Barrera, C. R., Valenzuela, A., & Videla, L. A. (2016) · Nutrition · doi:10.1016/j.nut.2016.04.006 · Cited by 87
Potato tuber expression of Arabidopsis WRINKLED1 increase triacylglycerol and membrane lipids while affecting central carbohydrate metabolism
Hofvander, P., Ischebeck, T., Turesson, H., Kushwaha, S. K., Feußner, I., Carlsson, A. S., & Andersson, M. (2016) · Plant Biotechnology Journal · doi:10.1111/pbi.12550 · Cited by 83
Ultrasonic nanoemulsification of food grade trans-cinnamaldehyde: 1,8-Cineol and investigation of the mechanism of antibacterial activity
Moghimi, R., Aliahmadi, A., & Rafati, H. (2016) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2016.10.020 · Cited by 83
Fatty acid composition and phospholipid types used in infant formulas modifies the establishment of human gut bacteria in germ-free mice
Nejrup, R. G., Licht, T. R., & Hellgren, L. I. (2017) · Scientific Reports · doi:10.1038/s41598-017-04298-0 · Cited by 88
Significance of dark CO2 fixation in arctic soils
Šantrůčková, H., Kotas, P., Bárta, J., Urich, T., Čapek, P., Palmtag, J., Alves, R. E., Biasi, C., Diáková, K., Gentsch, N., et al. (2018) · Soil Biology and Biochemistry · doi:10.1016/j.soilbio.2017.12.021 · Cited by 84
Soil Organic Carbon, Aggregation, and Microbial Community Structure in Annual and Perennial Biofuel Crops
McGowan, A. R., Nicoloso, R. D. S., Diop, H. E., Roozeboom, K. L., & Rice, C. W. (2018) · Agronomy Journal · doi:10.2134/agronj2018.04.0284 · Cited by 91
l -Serine dietary supplementation is associated with clinical improvement of loss-of-function GRIN2B -related pediatric encephalopathy
Soto, D., Olivella, M., Grau, C., Armstrong, J., Alcon, C., Gasull, X., Santos‐Gómez, A., Locubiche, S., de Salazar, M. G., García-Díaz, R., et al. (2019) · Science Signaling · doi:10.1126/scisignal.aaw0936 · Cited by 86
Decreased surfactant lipids correlate with lung function in chronic obstructive pulmonary disease (COPD)
Agudelo, C. W., Kumley, B., Área-Gómez, E., Xu, Y., Dabo, A. J., Geraghty, P. M., Campos, M., Foronjy, R., & García‐Arcos, I. (2020) · PLoS ONE · doi:10.1371/journal.pone.0228279 · Cited by 85
“Lipid extracts were prepared using a modified Bligh and Dyer method [26], spiked with a cocktail of internal standards, and analyzed using a 6490 Triple Quadrupole LC/MS system (Agilent Technologies, Santa Clara, CA).”
Method
Metabolic control analysis of hepatic glycogen synthesis in vivo
Nozaki, Y., Petersen, M. C., Zhang, D., Vatner, D. F., Perry, R. J., Abulizi, A., Hædersdal, S., Zhang, X., Butrico, G. M., Samuel, V. T., et al. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1921694117 · Cited by 85
“Liver UDP-glucose was measured by liquid chromatography MS/MS. Liver triglycerides were extracted by the method of Bligh and Dyer (43) and measured using a colorimetric assay (Sekisui).”
MethodInfluential
Bioremediation and biomass production of microalgae cultivation in river water contaminated with pharmaceutical effluent
Singh, A., Ummalyma, S. B., & Sahoo, D. (2020) · Bioresource Technology · doi:10.1016/j.biortech.2020.123233 · Cited by 82
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Höring, M., Ejsing, C. S., Krautbauer, S., et al. (2021) · Journal of Lipid Research · doi:10.1016/j.jlr.2021.100050 · Cited by 83
“The samples were extracted according to the procedure described by Bligh and Dyer (22) with a total chloroform volume of 2 ml.”
Method
Pharmacological treatment with FGF21 strongly improves plasma cholesterol metabolism to reduce atherosclerosis
Liu, C., Schönke, M., Zhou, E., Li, Z., Kooijman, S., Boon, M. R., Larsson, M., Wallenius, K., Dekker, N., Barlind, L., et al. (2021) · Cardiovascular Research · doi:10.1093/cvr/cvab076 · Cited by 81
“Hepatic lipids were extracted from frozen liver samples using a modified protocol from Bligh and Dyer.31 Using commercial kits, TG, TC, and phospholipid (PL) (Instruchemie, Delfzijl, The Netherlands) and protein (Pierce, Thermo Fisher Scientific, Waltham, MA, USA) concentrations were determined.”
Method
Antioxidant peptides derived from potato, seaweed, microbial and spinach proteins: Oxidative stability of 5% fish oil-in-water emulsions
Yeşiltaş, B., García‐Moreno, P. J., Echers, S. G., Olsen, T. H., Jones, N. C., Hoffmann, S. V., Marcatili, P., Overgaard, M. T., Hansen, E. B., & Jacobsen, C. (2022) · Food Chemistry · doi:10.1016/j.foodchem.2022.132699 · Cited by 77
Identification of an alternative triglyceride biosynthesis pathway
McLelland, G., López-Osias, M., Verzijl, C. R. C., Ellenbroek, B. D., Oliveira, R. A., Boon, N. J., Dekker, M., van den Hengel, L. G., Ali, R. B., Janssen, H., et al. (2023) · Nature · doi:10.1038/s41586-023-06497-4 · Cited by 87
Lipid and fatty acid content in cultivated live feed organisms compared to marine copepods
Evjemo, J. O., & Ôlsen, Y. (1997) · Springer eBooks · doi:10.1007/978-94-017-2097-7_24 · Cited by 83
Effects of the commercial probiotic Lactobacillus casei on the growth, protein content of skin mucus and stress resistance of juveniles of the Porthole livebearer Poecilopsis gracilis (Poecilidae)
Hernández, L. H. H., Barrera, T. C., Mejía, J. C., et al. (2009) · Aquaculture Nutrition · doi:10.1111/j.1365-2095.2009.00679.x · Cited by 83
Ecological Tracers Can Quantify Food Web Structure and Change
Hebert, C. E., Arts, M. T., & Weseloh, D. V. C. (2006) · Environmental Science & Technology · doi:10.1021/es0520619 · Cited by 82
Effects of (−)-epicatechin on a diet-induced rat model of cardiometabolic risk factors
Gutiérrez-Salmeán, G., Ortiz-Vilchis, P., Vacaseydel, C. M., Garduño‐Siciliano, L., Chamorro, G., Meaney, E., Villafaña, S., Villarreal, F. J., Ceballos, G. M., & Ramírez‐Sánchez, I. (2014) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2014.01.053 · Cited by 82
Sphingolipid Transport to the Apical Plasma Membrane Domain in Human Hepatoma Cells Is Controlled by PKC and PKA Activity: A Correlation with Cell Polarity in HepG2 Cells
Zegers, M. M., & Hoekstra, D. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.138.2.307 · Cited by 80

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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