Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 9,501–9,600 of 48,846

Chemical, microbiological and sensory changes associated with fish sauce processing
Kılınç, B., Çaklı, Ş., Tolasa, S., et al. (2005) · European Food Research and Technology · doi:10.1007/s00217-005-0198-4 · Cited by 65
Secondary sphingolipid accumulation in a macrophage model of Gaucher disease
Hein, L. K., Meikle, P. J., Hopwood, J. J., et al. (2007) · Molecular Genetics and Metabolism · doi:10.1016/j.ymgme.2007.08.001 · Cited by 65
Spatio-temporal variations in biological performances and summer mortality of the Pacific oyster Crassostrea gigas in Normandy (France)
Costil, K., Royer, J., Ropert, M., et al. (2005) · Helgoland Marine Research · doi:10.1007/s10152-005-0004-5 · Cited by 65
Utilization of a submersible UV fluorometer for monitoring anthropogenic inputs in the Mediterranean coastal waters
Tedetti, M., Guigue, C., & Goutx, M. (2009) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2009.10.018 · Cited by 65
Development and Evaluation of Condition Indices for the Lake Whitefish
Rennie, M. D., & Verdon, R. (2008) · North American Journal of Fisheries Management · doi:10.1577/m06-258.1 · Cited by 65
Rosiglitazone Controls Fatty Acid Cycling in Human Adipose Tissue by Means of Glyceroneogenesis and Glycerol Phosphorylation
Leroyer, S., Tordjman, J., Chauvet, G., Quette, J., Chapron, C., Forest, C. D., & Antoine, B. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m512943200 · Cited by 65
Characteristics of meat batters with added native and preheated defatted walnut
Cofrades, S., Serrano, A., Ayo, J., et al. (2007) · Food Chemistry · doi:10.1016/j.foodchem.2007.10.006 · Cited by 65
Effects of dietary phosphorus and phospholipid level on growth, and phosphorus deficiency signs in juvenile Japanese flounder, Paralichthys olivaceus
Uyan, O., Koshio, S., Ishikawa, M., et al. (2007) · Aquaculture · doi:10.1016/j.aquaculture.2007.01.020 · Cited by 65
Algal biofuel: bountiful lipid from Chlorococcum sp. proliferating in municipal wastewater
Mahapatra, D. M., & Ramachandra, T. V. (2013) · NOT FOUND REPOSITORY (Indian Institute of Science Bangalore) · Cited by 65
Microbial community structure and nutrient availability in oil sands reclaimed boreal soils
MacKenzie, M. D., & Quideau, S. A. (2009) · Applied Soil Ecology · doi:10.1016/j.apsoil.2009.09.002 · Cited by 64
How to study lipidomes
Seppänen‐Laakso, T., & Orešič, M. (2008) · Journal of Molecular Endocrinology · doi:10.1677/jme-08-0150 · Cited by 65
Three extraction methods for determination of lipids in fish meal: Evaluation of a hexane/isopropanol method as an alternative to chloroform‐based methods
Gunnlaugsdóttir, H., & ACKMAN, R. G. (1993) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.2740610216 · Cited by 65
Aerobic kinetoplastid flagellate Phytomonas does not require heme for viability
Kořený, L., Sobotka, R., Kovářová, J., Gnipová, A., Flegontov, P., Horváth, A., Obornı́k, M., Ayala, F. J., & Lukeš, J. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1201089109 · Cited by 65
Effects of modification of membrane lipid composition onBacillus subtilissporulation and spore properties
Griffiths, K. K., & Setlow, P. (2009) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2009.04176.x · Cited by 65
LYCAT, a homologue of C. elegans acl-8,acl-9, and acl-10, determines the fatty acid composition of phosphatidylinositol in mice
Imae, R., Inoue, T., Nakasaki, Y., Uchida, Y., Ohba, Y., Kono, N., Nakanishi, H., Sasaki, T., Mitani, S., & Arai, H. (2011) · Journal of Lipid Research · doi:10.1194/jlr.m018655 · Cited by 64
Cloning and Characterization of the Gene Encoding the Major Cell-Associated Phospholipase A of Legionella pneumophila , plaB , Exhibiting Hemolytic Activity
Flieger, A., Rydzewski, K., Banerji, S., Broich, M., & Heuner, K. (2004) · Infection and Immunity · doi:10.1128/iai.72.5.2648-2658.2004 · Cited by 65
Olive leaves offer more than phenolic compounds – Fatty acids and mineral composition of varieties from Southern Brazil
Cavalheiro, C. V., Picoloto, R. S., Cichoski, A. J., Wagner, R. C., de Menezes, C. R., Zepka, L. Q., Croce, D. M. D., & Barin, J. S. (2015) · Industrial Crops and Products · doi:10.1016/j.indcrop.2015.03.054 · Cited by 66
Comparison of the performances of Nero Siciliano pigs reared indoors and outdoors: 2. Joints composition, meat and fat traits
Pugliese, C., Calagna, G., Chiofalo, V., et al. (2004) · Meat Science · doi:10.1016/j.meatsci.2004.02.020 · Cited by 65
Triacylglycerol lipolysis is linked to sphingolipid and phospholipid metabolism of the yeast Saccharomyces cerevisiae☆
Rajakumari, S., Rajasekharan, R., & Daum, G. (2010) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2010.08.004 · Cited by 64
CO2 assimilation in a thermophilic cyanobacterium
Miyairi, S. (1995) · Energy Conversion and Management · doi:10.1016/0196-8904(95)00116-u · Cited by 65
Input related microbial carbon dynamic of soil organic matter in particle size fractions
Gude, A., Kandeler, E., & Gleixner, G. (2012) · Soil Biology and Biochemistry · doi:10.1016/j.soilbio.2012.01.003 · Cited by 69
Modelling and predicting the simultaneous growth of Listeria monocytogenes and psychrotolerant lactic acid bacteria in processed seafood and mayonnaise-based seafood salads
Mejlholm, O., & Dalgaard, P. (2014) · Food Microbiology · doi:10.1016/j.fm.2014.07.005 · Cited by 65
Fat-specific satiety in humans for fat high in linoleic acid vs fat high in oleic acid
Kamphuis, M. M. J. W., Westerterp‐Plantenga, M. S., & Saris, W. H. M. (2001) · European Journal of Clinical Nutrition · doi:10.1038/sj.ejcn.1601222 · Cited by 65
An ex vivo human skin model for studying skin barrier repair
Danso, M. O., Berkers, T., Mieremet, A., Hausil, F., & Bouwstra, J. A. (2014) · Experimental Dermatology · doi:10.1111/exd.12579 · Cited by 66
Preference and juiciness of Iberian dry-cured loin as affected by intramuscular fat content, crossbreeding and rearing system
Ventanas, S., Ruiz, J., Garcı́a, C., et al. (2007) · Meat Science · doi:10.1016/j.meatsci.2007.04.001 · Cited by 65
Gastric conditions control both the evolution of the organization of protein-stabilized emulsions and the kinetic of lipolysis during in vitro digestion
Kenmogne-Domguia, H. B., Meynier, A., Viau, M., et al. (2012) · Food & Function · doi:10.1039/c2fo30031a · Cited by 65
Germination, morpho-physiological and biochemical responses of coriander (Coriandrum sativum L.) to zinc excess
Marichali, A., Dallali, S., Ouerghemmi, S., Sebei, H., & Hosni, K. (2014) · Industrial Crops and Products · doi:10.1016/j.indcrop.2014.02.033 · Cited by 66
tBid induces alterations of mitochondrial fatty acid oxidation flux by malonyl-CoA-independent inhibition of carnitine palmitoyltransferase-1
Giordano, A., Calvani, M., Petillo, O., Grippo, P., Tuccillo, F. M., Melone, M. A. B., Bonelli, P., Calarco, A., & Peluso, G. (2005) · Cell Death and Differentiation · doi:10.1038/sj.cdd.4401636 · Cited by 68
Trans-intestinal cholesterol efflux is not mediated through high density lipoprotein
Vrins, C. L. J., Ottenhoff, R., van den Oever, K., de Waart, D. R., Kruijt, J. K., Zhao, Y., van Berkel, T. J. C., Havekes, L. M., Aerts, J. M. F. G., Eck, M. V., et al. (2012) · Journal of Lipid Research · doi:10.1194/jlr.m022194 · Cited by 68
Resveratrol inhibits fatty acid and triacylglycerol synthesis in rat hepatocytes
Gnoni, G. V., & Paglialonga, G. (2009) · European Journal of Clinical Investigation · doi:10.1111/j.1365-2362.2008.02077.x · Cited by 65
Lipid extraction has little effect on the δ15N of aquatic consumers
Ingram, T., Matthews, B., Harrod, C., et al. (2007) · Limnology and Oceanography Methods · doi:10.4319/lom.2007.5.338 · Cited by 65
Modern organic and broiler chickens sold for human consumption provide more energy from fat than protein
Wang, Y., Lehane, C., Ghebremeskel, K., & Crawford, M. A. (2009) · Public Health Nutrition · doi:10.1017/s1368980009991157 · Cited by 64
Maturation-associated alterations of the biochemical characteristics of the black truffle Tuber melanosporum Vitt.
Harki, E., Bouya, D., & Dargent, R. (2005) · Food Chemistry · doi:10.1016/j.foodchem.2005.08.030 · Cited by 65
Shelf life of several marine fish species of Bangladesh during ice storage
Reza, M. S., Bapary, M. A. J., Ahasan, C. T., et al. (2008) · International Journal of Food Science & Technology · doi:10.1111/j.1365-2621.2007.01613.x · Cited by 65
A multiple-scale analysis of metazoan meiofaunal distribution in the deep Mediterranean Sea
Gambi, C., & Danovaro, R. (2006) · Deep Sea Research Part I Oceanographic Research Papers · doi:10.1016/j.dsr.2006.05.003 · Cited by 66
Interactions between α-Tocopherol and Rosmarinic Acid and Its Alkyl Esters in Emulsions: Synergistic, Additive, or Antagonistic Effect?
Panya, A., Kittipongpittaya, K., Laguerre, M., et al. (2012) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf302673j · Cited by 65
Characterization of enzymatic hydrolyzed snow crab (Chionoecetes opilio) by-product fractions: A source of high-valued biomolecules
Beaulieu, L., Thibodeau, J., Bryl, P., et al. (2009) · Bioresource Technology · doi:10.1016/j.biortech.2009.01.073 · Cited by 65
Halomicrobium mukohataei gen. nov., comb. nov., and emended description of Halomicrobium mukohataei.
Oren, A., Elevi, R., Watanabe, S., et al. (2002) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/00207713-52-5-1831 · Cited by 65
Fate of macroalgae in benthic systems: carbon and nitrogen cycling within the microbial community
Hardison, A. K., Canuel, E. A., Anderson, I., et al. (2010) · Marine Ecology Progress Series · doi:10.3354/meps08720 · Cited by 65
ACAT2 contributes cholesteryl esters to newly secreted VLDL, whereas LCAT adds cholesteryl ester to LDL in mice
Lee, R. G., Shah, R., Sawyer, J. K., Hamilton, R. L., Parks, J. S., & Rudel, L. L. (2005) · Journal of Lipid Research · doi:10.1194/jlr.m500018-jlr200 · Cited by 65
Soy protein concentrate as a protein source for Senegalese sole (Solea senegalensisKaup 1858) diets: effects on growth and amino acid metabolism of postlarvae
Aragão, C., Conceição, L. E. C., Dias, J. P., et al. (2003) · Aquaculture Research · doi:10.1111/j.1365-2109.2003.00971.x · Cited by 65
Molecular Analysis of Phr Peptide Processing inBacillus subtilis
Stephenson, S., Mueller, C., Jiang, M., et al. (2003) · Journal of Bacteriology · doi:10.1128/jb.185.16.4861-4871.2003 · Cited by 65
Genome-Driven Investigation of Compatible Solute Biosynthesis Pathways ofPseudomonas syringaepv. syringae and Their Contribution to Water Stress Tolerance
Kurz, M., Burch, A. Y., Seip, B., et al. (2010) · Applied and Environmental Microbiology · doi:10.1128/aem.00686-10 · Cited by 65
Activation of Dendritic Cells by Liposomes Prepared from Phosphatidylinositol Mannosides fromMycobacterium bovisBacillus Calmette-Guerin and Adjuvant Activity In Vivo
Sprott, G. D., Dicaire, C. J., Gurnani, K., et al. (2004) · Infection and Immunity · doi:10.1128/iai.72.9.5235-5246.2004 · Cited by 65
Response surface methodology applied to the analysis of rice bran oil extraction process with ethanol
Rodrigues, C. E. D. C., & Oliveira, R. (2010) · International Journal of Food Science & Technology · doi:10.1111/j.1365-2621.2010.02202.x · Cited by 65
Differential effects of cholesterol and 7-dehydrocholesterol on the ligand binding activity of the hippocampal serotonin1A receptor: Implications in SLOS
Singh, P., Paila, Y. D., & Chattopadhyay, A. (2007) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2007.04.135 · Cited by 67
Capsular Polysaccharide Surrounds Smooth and Rugose Types ofSalmonella entericaserovar Typhimurium DT104
de Rezende, C. E., Anriany, Y., Carr, L. E., et al. (2005) · Applied and Environmental Microbiology · doi:10.1128/aem.71.11.7345-7351.2005 · Cited by 65
Reproductive period affects lipid composition and quality of fresh and stored spermatozoa in Turkeys
Douard, V., Hermier, D., Magistrini, M., & Blesbois, É. (2003) · Theriogenology · doi:10.1016/s0093-691x(02)01086-5 · Cited by 65
Seasonal changes of fatty acids of cuttlefish L. (Mollusca: Cephalopoda) in the north eastern Mediterranean sea
Özyurt, G., Duysak, Ö., Akamca, E., & Türeli̇, C. (2005) · Food Chemistry · doi:10.1016/j.foodchem.2004.12.044 · Cited by 65
Nutritional composition of the meat of Hereford and Braford steers finished on pastures or in a feedlot in southern Brazil
de Freitas, A. K., Lobato, J. F. P., Cardoso, L. L., Tarouco, J. U., Vieira, R. M., Dillenburg, D. R., & Castro, I. (2013) · Meat Science · doi:10.1016/j.meatsci.2013.07.021 · Cited by 69
Human and Bovine Milk Gangliosides Differ in Their Fatty Acid Composition
Bode, L., Beermann, C., Mank, M., Kohn, G., & Boehm, G. (2004) · Journal of Nutrition · doi:10.1093/jn/134.11.3016 · Cited by 66
Microsomal antiestrogen-binding site ligands induce growth control and differentiation of human breast cancer cells through the modulation of cholesterol metabolism
Payré, B., de Médina, P., Boubekeur, N., Mhamdi, L., Bertrand‐Michel, J., Tercé, F., Fourquaux, I., Goudounèche, D., Record, M., Poirot, M., et al. (2008) · Molecular Cancer Therapeutics · doi:10.1158/1535-7163.mct-08-0507 · Cited by 65
Enhancement of Lipid Extraction from Marine Microalga,ScenedesmusAssociated with High-Pressure Homogenization Process
Cho, S.-C., Choi, W.-Y., Oh, S.-H., Lee, C.-G., Seo, Y.-C., Kim, J., Song, C.-H., Kim, G.-V., Lee, S.-Y., Kang, D.-H., et al. (2012) · BioMed Research International · doi:10.1155/2012/359432 · Cited by 65
Diet development and evaluation for juvenile abalone, Haliotis asinina Linne: Lipid and essential fatty acid levels
Bautista-Teruel, M. N., Koshio, S., & Ishikawa, M. (2011) · Aquaculture · doi:10.1016/j.aquaculture.2011.01.004 · Cited by 65
Enhancement of lipid productivity of Rhodosporidium toruloides in distillery wastewater by increasing cell density
Ling, J., Nip, S., & Shim, H. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2013.07.023 · Cited by 65
Lipids from stomach oil of procellariiform seabirds document the importance of myctophid fish in the Southern Ocean
Connan, M., Cherel, Y., & Mayzaud, P. (2007) · Limnology and Oceanography · doi:10.4319/lo.2007.52.6.2445 · Cited by 65
Two Pathways of Sphingolipid Biosynthesis Are Separated in the Yeast Pichia pastoris
Ternes, P., Wobbe, T., Schwarz, M., Albrecht, S., Feussner, K., Riezman, I., Cregg, J. M., Heinz, E., Riezman, H., Feußner, I., et al. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.193094 · Cited by 66
Nutritional composition and physicochemical properties of two green seaweeds (Ulva pertusa and U. intestinalis) from the Pattani Bay in Southern Thailand
Benjama, O., & Masniyom, P. (2011) · DOAJ (DOAJ: Directory of Open Access Journals) · Cited by 65
Trehalose Polyphleates Are Produced by a Glycolipid Biosynthetic Pathway Conserved across Phylogenetically Distant Mycobacteria
Burbaud, S., Laval, F., Lemassu, A., Daffé, M., Guilhot, C., & Chalut, C. (2016) · Cell chemical biology · doi:10.1016/j.chembiol.2015.11.013 · Cited by 64
Ontogenetic dietary changes of green turtles (Chelonia mydas) in the temperate southwestern Atlantic
Vélez‐Rubio, G. M., Cardona, L., López‐Mendilaharsu, M., et al. (2016) · Marine Biology · doi:10.1007/s00227-016-2827-9 · Cited by 65
Beneficial changes in biomass and lipid of microalgae Anabaena variabilis facing the ultrasonic stress environment
Han, F., Pei, H., Hu, W., Jiang, L., Cheng, J., & Zhang, L. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2016.02.103 · Cited by 64
Reuse of microalgae grown in full-scale wastewater treatment ponds: Thermochemical pretreatment and biogas production
Passos, F. L. D. R., Felix, L., Rocha, H., Pereira, J. D. O., & de Aquino, S. F. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2016.03.006 · Cited by 65
Skeletal growth, respiration rate and fatty acid composition in the cold-water coral Lophelia pertusa under varying food conditions
Larsson, A., Lundälv, T., & van Oevelen, D. (2013) · Marine Ecology Progress Series · doi:10.3354/meps10284 · Cited by 65
Uptake, tissue distribution and depuration of triclosan in the guppy Poecilia vivipara acclimated to freshwater
Escarrone, A. L. V., Caldas, S. S., Prímel, E. G., et al. (2016) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2016.04.039 · Cited by 65
Levels of polyunsaturated fatty acids correlate with growth rate in plant cell cultures
Meï, C., Michaud, M., Cussac, M., et al. (2015) · Scientific Reports · doi:10.1038/srep15207 · Cited by 65
Preparation of derivatives of fatty acids
Christie, W. W., & Han, X. (2012) · Elsevier eBooks · doi:10.1533/9780857097866.145 · Cited by 68
Antimicrobial and antioxidant activity of liquid smoke and its potential application to bacon
Soares, J. M., da Silva, P. F., Puton, B. M. S., et al. (2016) · Innovative Food Science & Emerging Technologies · doi:10.1016/j.ifset.2016.10.007 · Cited by 65
The acyltransferase LYCAT controls specific phosphoinositides and related membrane traffic
Bone, L. N., Dayam, R. M., Lee, M., Kono, N., Fairn, G. D., Arai, H., Botelho, R. J., & Antonescu, C. N. (2016) · Molecular Biology of the Cell · doi:10.1091/mbc.e16-09-0668 · Cited by 59
Schistosomal-derived lysophosphatidylcholine triggers M2 polarization of macrophages through PPARγ dependent mechanisms
Assunção, L. S., Magalhães, K. G., Carneiro, A. B., Molinaro, R., de Almeida, P. E., Atella, G. C., Castro-Faria-Neto, H. C., & Bozza, P. T. (2016) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2016.11.006 · Cited by 69
Omental adipocytes enhance the invasiveness of gastric cancer cells by oleic acid-induced activation of the PI3K-Akt signaling pathway
Xiang, F., Wu, K., Liu, Y., et al. (2016) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2016.12.002 · Cited by 65
Enhanced extraction of lipids from microalgae with eco-friendly mixture of methanol and ethyl acetate for biodiesel production
Wu, J., Alam, M. A., Pan, Y., Huang, D., Wang, Z., & Wang, T. (2017) · Journal of the Taiwan Institute of Chemical Engineers · doi:10.1016/j.jtice.2016.12.039 · Cited by 64
Mechanism for leptin’s acute insulin-independent effect to reverse diabetic ketoacidosis
Perry, R. J., Peng, L., Abulizi, A., Kennedy, L., Cline, G. W., & Shulman, G. I. (2017) · Journal of Clinical Investigation · doi:10.1172/jci88477 · Cited by 68
FABP1 knockdown in human enterocytes impairs proliferation and alters lipid metabolism
Sawicki, L. R., Arias, N. M. B., Scaglia, N., et al. (2017) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2017.09.006 · Cited by 65
AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity
Luo, H., Ming, J., Lian, G., Liu, Q., Shi, M., Li, T. Y., Song, L., Ye, J., He, Y., Yao, L., et al. (2018) · Cell Metabolism · doi:10.1016/j.cmet.2018.02.021 · Cited by 66
Discovery of Lipidome Alterations Following Traumatic Brain Injury via High-Resolution Metabolomics
Hogan, S., Phan, J. H., Alvarado‐Velez, M., Wang, M. D., Bellamkonda, R. V., Fernández, F. M., & LaPlaca, M. C. (2018) · Journal of Proteome Research · doi:10.1021/acs.jproteome.8b00068 · Cited by 68
Supplementation with curcumin inhibits intestinal cholesterol absorption and prevents atherosclerosis in high-fat diet–fed apolipoprotein E knockout mice
Zou, J., Zhang, S., Li, P., Zheng, X., & Feng, D. (2018) · Nutrition Research · doi:10.1016/j.nutres.2018.04.017 · Cited by 67
C-4 sterol demethylation enzymes distinguish bacterial and eukaryotic sterol synthesis
Lee, A. K., Banta, A. B., Wei, J. H., Kiemle, D. J., Feng, J., Giner, J., & Welander, P. V. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1802930115 · Cited by 63
Biochemical composition and physicochemical properties of two red seaweeds (Gracilaria fisheri and G. tenuistipitata) from the Pattani Bay in Southern Thailand
Masniyom, P., & Benjama, O. (2012) · DOAJ (DOAJ: Directory of Open Access Journals) · Cited by 64
Substitution of linoleic acid with α-linolenic acid or long chain n-3 polyunsaturated fatty acid prevents Western diet induced nonalcoholic steatohepatitis
Jeyapal, S., Kona, S. R., Mullapudi, S. V., Putcha, U. K., Gurumurthy, P., & Ibrahim, A. (2018) · Scientific Reports · doi:10.1038/s41598-018-29222-y · Cited by 68
Classification of Chicken Parts Using a Portable Near-Infrared (NIR) Spectrophotometer and Machine Learning
Pérez, I. M. N., Badaró, A. T., Barbon, S., et al. (2018) · Applied Spectroscopy · doi:10.1177/0003702818788878 · Cited by 65
Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
Rodríguez, J. P., Guijas, C., Astudillo, A. M., et al. (2019) · Cancers · doi:10.3390/cancers11040524 · Cited by 65
Selective Extraction of ω-3 Fatty Acids from Nannochloropsis sp. Using Supercritical CO2 Extraction
Leone, G. P., Balducchi, R., Mehariya, S., Martino, M., Larocca, V., Sanzo, G. D., Iovine, A., Casella, P., Marino, T., Karatza, D., et al. (2019) · Molecules · doi:10.3390/molecules24132406 · Cited by 69
Ozonation of Brazil nuts: Decomposition kinetics, control of Aspergillus flavus and the effect on color and on raw oil quality
Oliveira, J. M., de Alencar, E. R., Blum, L. E. B., et al. (2020) · LWT · doi:10.1016/j.lwt.2020.109106 · Cited by 65
Development and cost-benefit analysis of a novel process for biofuel production from microalgae using pre-treated high-strength fresh cheese whey wastewater
Pandey, A., Srivastava, S., & Kumar, S. (2020) · Environmental Science and Pollution Research · doi:10.1007/s11356-020-08535-4 · Cited by 66
“The total lipid was extracted from oven-dried biomass as per modified Bligh and Dyer (1959) method.”
Method
Mass spectrometry-based untargeted metabolomics approach for differentiation of beef of different geographic origins
Man, K.-Y., Chan, C.-O., Tang, H.-H., Dong, N., Capozzi, F., Wong, K., Kwok, K. W. H., Chan, H. M., & Mok, D. K. (2020) · Food Chemistry · doi:10.1016/j.foodchem.2020.127847 · Cited by 65
Selective recovery and purification of carotenoids and fatty acids from Rhodotorula glutinis using mixtures of biosolvents
Mussagy, C. U., Remonatto, D., de Paula, A. V., Herculano, R. D., Santos‐Ebinuma, V. C., Coutinho, J. A. P., & Pereira, J. F. B. (2021) · Separation and Purification Technology · doi:10.1016/j.seppur.2021.118548 · Cited by 68
“glutinis cells, intracellular lipids were extracted from the (1) fresh untreated wet biomass, (2) pre-treated (with one freeze/thaw cycle) wet biomass, and (3) pre-treated (with one freeze/thaw cycle) dry biomass, further dried and weighed by using a modified Bligh and Dyer procedure [10].”
Method
In-vitro antioxidant and antibacterial properties of fermentatively and enzymatically prepared chicken liver protein hydrolysates
Chakka, A. K., Elias, M., Jini, R., et al. (2015) · Journal of Food Science and Technology · doi:10.1007/s13197-015-1920-2 · Cited by 65
Sustained submaximal exercise does not alter the integrity of the lung blood-gas barrier in elite athletes
Hopkins, S. R., Schoene, R. B., Henderson, W. R., et al. (1998) · Journal of Applied Physiology · doi:10.1152/jappl.1998.84.4.1185 · Cited by 65
Formic acid is a product of the α-oxidation of fatty acids by human skin fibroblasts: deficiency of formic acid production in peroxisome-deficient fibroblasts
Poulos, A., Sharp, P. C., Singh, H., Johnson, D. W., Carey, W. F., & Easton, C. J. (1993) · Biochemical Journal · doi:10.1042/bj2920457 · Cited by 65
FcγRIIA requires a Gi-dependent pathway for an efficient stimulation of phosphoinositide 3-kinase, calcium mobilization, and platelet aggregation
Gratacap, M., Hérault, J.-P., Viala, C., et al. (2000) · Blood · doi:10.1182/blood.v96.10.3439 · Cited by 65
Degradation of petroleum model compounds immobilized on clay by a hypersaline microbial mat
Grötzschel, S., Köster, J., Abed, R. M. M., & de Beer, D. (2002) · Biodegradation · doi:10.1023/a:1021263009377 · Cited by 65
Lipids of human retina, retinal pigment epithelium, and Bruch's membrane/choroid: comparison of macular and peripheral regions.
Gülcan, H., Alvarez, R., Maude, M. B., & Anderson, R. E. (1993) · PubMed · Cited by 65
Synergistic membrane interactions of the two C2 domains of synaptotagmin.
Damer, C. K., & Creutz, C. E. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47397-8 · Cited by 65
Role ofListeria monocytogenesExotoxins Listeriolysin and Phosphatidylinositol-Specific Phospholipase C in Activation of Human Neutrophils
Sibelius, U., Schulz, E.-C., Rose, F., et al. (1999) · Infection and Immunity · doi:10.1128/iai.67.3.1125-1130.1999 · Cited by 65
Upregulation of ceramide and its regulating mechanism in a rat model of chronic cerebral ischemia
Ohtani, R., Tomimoto, H., Kondo, T., Wakita, H., Akiguchi, I., Shibasaki, H., & Okazaki, T. (2004) · Brain Research · doi:10.1016/j.brainres.2004.07.024 · Cited by 65
Cholestasis and hypercholesterolemia in SCD1-deficient mice fed a low-fat, high-carbohydrate diet
Flowers, M. T., Groen, A. K., Oler, A. T., Keller, M. P., Choi, Y., Schueler, K. L., Richards, O. C., Hong, L., Miyazaki, M., Kuipers, F., et al. (2006) · Journal of Lipid Research · doi:10.1194/jlr.m600203-jlr200 · Cited by 65
Effect of dietary alpha-linolenate/linoleate balance on mean survival time, incidence of stroke and blood pressure of spontaneously hypertensive rats
Shimokawa, T., Moriuchi, A., Hori, T., et al. (1988) · Life Sciences · doi:10.1016/0024-3205(88)90356-6 · Cited by 65
Transacylase and Phospholipases in the Synthesis of Bis(monoacylglycero)phosphate
Amidon, B., Brown, A., & Waite, M. B. (1996) · Biochemistry · doi:10.1021/bi961164o · Cited by 67
Aldose Reductase-catalyzed Reduction of Aldehyde Phospholipids
Srivastava, S., Spite, M. R., Trent, J. O., West, M., Ahmed, Y., & Bhatnagar, A. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m403416200 · Cited by 65
Carnitine: a novel compatible solute in Lactobacillus plantarum
Kets, E., Galinski, E. A., & de Bont, J. A. M. (1994) · Archives of Microbiology · doi:10.1007/bf00301845 · Cited by 65

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks