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Total Internal Reflection Fluorescence
Abstract
Total internal reflection fluorescence (TIRF) is an optical effect particularly well-suited to the study of molecular and cellular phenomena at liquid/solid interfaces. Such interfaces are central to a wide range of biochemical and biophysical processes: binding to and triggering of ceils by hormones, neurotransmitters, and antigens; blood coagulation at foreign surfaces; electron transport in the mitochondrial membrane; adherence and mobility of bacteria, algae, and cultured animal cells to surfaces; and possible enhancement of reaction rates with cell surface receptors upon nonspecific adsorption and surface diffusion of an agonist. Liquid/solid interfaces also have important medical and industrial applications: e.g. detection of serum antibodies by surface immobilized antigens; and the manufacture of biochemical products by surface-immobilized enzymes. Total internal reflection spectroscopy for optical absorption studies (called attenuated total reflection or ATR) was developed somewhat earlier than the fluorescence applications and has been widely used in surface
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Cited by
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Petersen, J., Volz, J., & Rauschenbeutel, A. (2014) · Science · doi:10.1126/science.1257671 · Cited by 807
Supported planar membranes in studies of cell-cell recognition in the immune system
McConnell, H. M., Watts, T. H., Weis, R. M., & Brian, A. A. (1986) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(86)90016-x · Cited by 561
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Metadata
- Authors
- Axelrod, D., Burghardt, T. P., & Thompson, N. L.
- Venue
- Annual Review of Biophysics and Bioengineering
- Publisher
- Annual Reviews
- Published
- 1984-06
- Volume
- 13
- Issue
- 1
- Pages
- 247-268
- ISSN
- 0084-6589 (ISSN-L)
- OpenAlex
- W2096963388
- Cited by
- 741
- References
- 0
- FWCI
- 8.29
- Type
- journal article
- Language
- en
- Open access
- closed
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- Axelrod, D., Burghardt, T. P., & Thompson, N. L. (1984). Total Internal Reflection Fluorescence. Annual Review of Biophysics and Bioengineering. https://doi.org/10.1146/annurev.bb.13.060184.001335
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