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THE USE OF2H,13C,15N MULTIDIMENSIONAL NMR GTO STUDY THE STRUCTURE AND DYNAMICS OF PROTEINS
Abstract
During the past thirty years, deuterium labeling has been used to improve the resolution and sensitivity of protein NMR spectra used in a wide variety of applications. Most recently, the combination of triple resonance experiments and 2H, 13C, 15N labeled samples has been critical to the solution structure determination of several proteins with molecular weights on the order of 30 kDa. Here we review the developments in isotopic labeling strategies, NMR pulse sequences, and structure-determination protocols that have facilitated this advance and hold promise for future NMR-based structural studies of even larger systems. As well, we detail recent progress in the use of solution 2H NMR methods to probe the dynamics of protein sidechains.
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Cited by
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Luo, X., Tang, Z., Xia, G., Wassmann, K., Matsumoto, T., Rizo, J., & Yu, H. (2004) · Nature Structural & Molecular Biology · doi:10.1038/nsmb748 · Cited by 297
Investigating Protein–Ligand Interactions by Solution Nuclear Magnetic Resonance Spectroscopy
Becker, W., Bhattiprolu, K. C., Gubensäk, N., & Zangger, K. (2018) · ChemPhysChem · doi:10.1002/cphc.201701253 · Cited by 138
Newcombe, E. A., Ruff, K. M., Sethi, A., Ormsby, A. R., Ramdzan, Y. M., Fox, A., Purcell, A. W., Gooley, P. R., Pappu, R. V., & Hatters, D. M. (2018) · Journal of Molecular Biology · doi:10.1016/j.jmb.2018.03.031 · Cited by 66
Mealman, T. D., Bagai, I., Singh, P., Goodlett, D. R., Rensing, C., Zhou, H., Wysocki, V. H., & McEvoy, M. M. (2011) · Biochemistry · doi:10.1021/bi102012j · Cited by 53
Brath, U., Akke, M., Yang, D., Kay, L. E., & Mulder, F. A. A. (2006) · Journal of the American Chemical Society · doi:10.1021/ja0570279 · Cited by 57
Fenwick, R. B., Prasannan, S., Campbell, L. J., Nietlispach, D., Evetts, K. A., Camonis, J., Mott, H. R., & Owen, D. (2009) · Biochemistry · doi:10.1021/bi802129d · Cited by 47
Bishop, A. C., Torres-Montalvo, G., Kotaru, S., Mimun, K., & Wand, A. J. (2023) · Nature Communications · doi:10.1038/s41467-023-37219-z · Cited by 16
Metadata
- Authors
- Gardner, K. H., & Kay, L. E.
- Venue
- Annual Review of Biophysics and Biomolecular Structure
- Publisher
- Annual Reviews
- Published
- 1998-06
- Volume
- 27
- Issue
- 1
- Pages
- 357-406
- ISSN
- 1056-8700 (ISSN-L)
- 1545-4266
- OpenAlex
- W2158105833
- Cited by
- 598
- References
- 0
- FWCI
- 16.46
- Type
- journal article
- Language
- en
- Open access
- closed
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