Interactions between CusF and CusB Identified by NMR Spectroscopy and Chemical Cross-Linking Coupled to Mass Spectrometry

Mealman, Tiffany D.; Bagai, Ireena; Singh, Pragya; Goodlett, David R.; Rensing, Christopher; Zhou, Hongjun; Wysocki, Vicki H.; McEvoy, Megan M.

doi:10.1021/bi102012j

Interactions between CusF and CusB Identified by NMR Spectroscopy and Chemical Cross-Linking Coupled to Mass Spectrometry

The E. coli periplasmic proteins CusF and CusB, as part of the CusCFBA efflux system, aid in the resistance of elevated levels of copper and silver by direct metal transfer between the metallochaperone CusF and the membrane fusion protein CusB, before metal extrusion from the periplasm to the ext…