The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
R ATIOMETRIC S INGLE-M OLECULE S TUDIES OF F REELY D IFFUSING B IOMOLECULES
Abstract
▪ Abstract We outline recent developments in biological single-molecule fluorescence detection with particular emphasis on observations by ratiometric fluorescence resonance energy transfer (FRET) of biomolecules freely diffusing in solution. Single-molecule-diffusion methodologies were developed to minimize perturbations introduced by interactions between molecules and surfaces. Confocal microscopy is used in combination with sensitive detectors to observe bursts of photons from fluorescently labeled biomolecules as they diffuse through the focal volume. These bursts are analyzed to extract ratiometric observables such as FRET efficiency and polarization anisotropy. We describe the development of single-molecule FRET methodology and its application to the observation of the Förster distance dependence and the study of protein folding and polymer physics problems. Finally, we discuss future advances in data acquisition and analysis techniques that can provide a more complete picture of the accessible molecular information.
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Cites
From Levinthal to pathways to funnels
Dill, K. A., & Chan, H. S. (1997) · Nature Structural & Molecular Biology · doi:10.1038/nsb0197-10 · Cited by 2,293
THEORY OF PROTEIN FOLDING: The Energy Landscape Perspective
Onuchic, J. N., Luthey-Schulten, Z., & Wolynes, P. G. (1997) · Annual Review of Physical Chemistry · doi:10.1146/annurev.physchem.48.1.545 · Cited by 2,210
Folding funnels and energy landscapes of larger proteins within the capillarity approximation
Wolynes, P. G. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.12.6170 · Cited by 218
Technological advances in high-throughput screening
Fernandes, P. B. (1998) · Current Opinion in Chemical Biology · doi:10.1016/s1367-5931(98)80089-6 · Cited by 170
Cited by
Synthetische molekulare Motoren und mechanische Maschinen
Kay, E. R., Leigh, D. A., & Zerbetto, F. (2007) · Angewandte Chemie · doi:10.1002/ange.200504313 · Cited by 624
Shot-Noise Limited Single-Molecule FRET Histograms: Comparison between Theory and Experiments
Nir, E., Michalet, X., Hamadani, K. M., Laurence, T. A., Neuhauser, D., Kovchegov, Y., & Weiss, S. (2006) · The Journal of Physical Chemistry B · doi:10.1021/jp063483n · Cited by 314
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Metadata
- Authors
- Deniz, A. A., Laurence, T. A., Dahan, M., Chemla, D. S., Schultz, P. G., & Weiss, S.
- Venue
- Annual Review of Physical Chemistry
- Publisher
- Annual Reviews
- Published
- 2001-10
- Volume
- 52
- Issue
- 1
- Pages
- 233-253
- ISSN
- 0066-426X (ISSN-L)
- 1545-1593
- OpenAlex
- W2098533425
- Cited by
- 204
- References
- 4
- FWCI
- 0.82
- Type
- journal article
- Language
- en
- Open access
- closed
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- Deniz, A. A., Laurence, T. A., Dahan, M., Chemla, D. S., Schultz, P. G., & Weiss, S. (2001). R ATIOMETRIC S INGLE-M OLECULE S TUDIES OF F REELY D IFFUSING B IOMOLECULES. Annual Review of Physical Chemistry. https://doi.org/10.1146/annurev.physchem.52.1.233
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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