Cited by 148Nanoparticle-Based Drug DeliveryCharacterization and Applications of Magnetic NanoparticlesUltrasound and Hyperthermia Applications

Magnetic targeting of magnetoliposomes to solid tumors with MR imaging monitoring in mice: feasibility

Fortin-Ripoche, Jean-Paul, Martina, Marie Sophie, Gazeau, Florence, Ménager, Christine, Wilhelm, Claire, Bacri, Jean-Claude, Lesieur, Sylviane, Clément, Olivier·2006·Radiology

Abstract

PURPOSE: To establish the feasibility of magnetoliposome tumor targeting with an extracorporeal magnet. MATERIALS AND METHODS: Animal experiments were performed in compliance with Institut National de la Santé Et de la Recherche Médicale animal protection guidelines and were approved by local government authorities. Magnetophoresis was used to measure the velocity of magnetoliposomes constituted of polyethylene glycol-lipids and anionic maghemite nanocrystals in a calibrated magnetic field in vitro. For in vivo studies, 38 male Swiss nude mice bearing a PC3 human prostate carcinoma tumor in each flank received an intravenous injection of magnetoliposomes (n = 27), saline (n = 9), or nonencapsulated superparamagnetic particles (n = 2) after a small magnet with a magnetic field of 0.3 T and a field gradient of 11 T/m was fixed to the skin above one tumor. The animals were examined at magnetic resonance (MR) imaging with eight different sequences, iron doses (13 mice), and magnet-application durations (12 mice). Their excised tumors were then stained with Perls Prussian blue and hematoxylin-eosin and were examined histologically. With use of the paired Student t test, signal intensity, tumor surface enhancement, and number of stained cells were compared between the control and magnet-exposed tumors to determine significant differences (P </= .01). RESULTS: The mean magnetoliposome velocity ranged from 10 to 40 mum/sec when the magnetic field equaled 0.13 T and the field gradient equaled 25 T/m. At T1-weighted three-dimensional spoiled gradient-echo MR imaging in vivo, the tumor exposed to the magnet showed strong negative enhancement, -52%, compared with the -7% enhancement of the other tumor. Maximal enhancement occurred after 3 hours of magnet application. After 24 hours of magnet application, intracapillary iron particle accumulation was observed in the targeted tumors only. CONCLUSION: Magnetic targeting of sterically stabilized magnetoliposomes after they are intravenously injected is feasible in vivo.

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Crossref · 10.1148/radiol.2392042110

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Authors
Fortin-Ripoche, J.-P., Martina, M. S., Gazeau, F., Ménager, C., Wilhelm, C., Bacri, J.-C., Lesieur, S., & Clément, O.
Affiliations
Laboratoire Matière et Systèmes Complexes (FR)
Venue
Radiology
Published
2006-05
Volume
239
Issue
2
Pages
415-424
ISSN
  • 0033-8419 (ISSN-L)
  • 1527-1315
OpenAlex
W2113807798
Cited by
148
References
46
Citations by year
2026: 1 · 2025: 3 · 2024: 2 · 2023: 4 · 2022: 6 · 2021: 4 · 2020: 8 · 2019: 3 · 2018: 4 · 2017: 9 · 2016: 9 · 2015: 8 · 2014: 4 · 2013: 11 · 2012: 13
FWCI
6.16
Type
journal article
Language
en
Open access
closed
Locations
  • Radiology (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
  • Physical Sciences › Engineering › Biomedical Engineering › Ultrasound and Hyperthermia Applications
Keywords
magnetoliposomes, magnetic targeting, magnetophoresis, solid tumors, prostate cancer, superparamagnetic iron oxide nanoparticles, magnetic resonance imaging, tumor targeting, iron accumulation, tumor enhancement, magnetic field gradient
MeSH terms
Animals, Drug Carriers, Feasibility Studies, Liposomes, Magnetic Resonance Imaging, Magnetics, Male, Mice, Nude, Neoplasms, Mice

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