The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Mechanical stimulation improves tissue-engineered human skeletal muscle
Abstract
Human bioartificial muscles (HBAMs) are tissue engineered by suspending muscle cells in collagen/MATRIGEL, casting in a silicone mold containing end attachment sites, and allowing the cells to differentiate for 8 to 16 days. The resulting HBAMs are representative of skeletal muscle in that they contain parallel arrays of postmitotic myofibers; however, they differ in many other morphological characteristics. To engineer improved HBAMs, i.e., more in vivo-like, we developed Mechanical Cell Stimulator (MCS) hardware to apply in vivo-like forces directly to the engineered tissue. A sensitive force transducer attached to the HBAM measured real-time, internally generated, as well as externally applied, forces. The muscle cells generated increasing internal forces during formation which were inhibitable with a cytoskeleton depolymerizer. Repetitive stretch/relaxation for 8 days increased the HBAM elasticity two- to threefold, mean myofiber diameter 12%, and myofiber area percent 40%. This system allows engineering of improved skeletal muscle analogs as well as a nondestructive method to determine passive force and viscoelastic properties of the resulting tissue.
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Cites
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Engineered In Vitro Disease Models
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Metadata
- Authors
- Powell, C. A., Smiley, B. L., Mills, J., & Vandenburgh, H. H.
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 2002-11-01
- Volume
- 283
- Issue
- 5
- Pages
- C1557-C1565
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- OpenAlex
- W2005840935
- Cited by
- 498
- References
- 34
- FWCI
- 6.05
- Type
- journal article
- Language
- en
- Open access
- closed
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